Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil

Abstract

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderma and evaluate their resistance profiles. Lesions from 25 dogs with pyoderma were sampled. Bacterial isolates were subjected to phenotypic and genotypic analysis for identification of the causative agent. S. pseudintermedius isolates were subjected to SmaI macrorestriction analysis and PFGE for genetic grouping, and PCR to identify the presence of the mecA gene. Their resistance profiles against 12 antimicrobials were also assessed. According to the microbiological analysis, 70 of the 75 isolates obtained were S. pseudintermedius. The isolates presented PFGE patterns, with similarity varying between 84.6 and 100%, and were grouped into 19 clusters. Despite a high frequency of mecA-positive isolates (66 out 70), only 12 presented resistances to oxacillin. Multi-resistance was identified in 29 isolates. The high frequency of MRSP isolated in this study highlights the relevance of identifying resistant strains to lead proper clinical treatment.Keywords: Staphylococcus pseudintermedius, canine pyoderma, mecA, PFGE, antimicrobial resistance RESUMO Staphylococcus pseudintermedius meticilina-resistente (MRSP) é de preocupação mundial na medicina veterinária. A identificação de cepas resistentes é necessária a um tratamento adequado e à prevenção da

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

“…Here, resistance to oxacillin by disk diffusion was detected in 13 of 44 strains (29,55%). However, PCR analysis for mecA revealed that seven out of the 13 strains classified as resistant to oxacillin by disk diffusion harbored the mecA gene and five strains that were positive for mecA (ELHASSAN et al, 2015;BOURGUIGNON et al, 2016). Although, mecA seems to be the most common gene associated with meticillin resistance in S. pseudintermedius, some studies have shown that another gene (mecC) could also be responsible for this resistance profile (KJELLMAN et al, 2015).…”

“…Amplicons were separated by electrophoresis on a 1.5% agarose gel with a 1-kb molecular marker (ThermoScientific ® Waltham, Massachusetts, USA). S. pseudintermedius MRSP 3279, a reference strain previously used in other studies (BANNOEHR et al, 2009;BOURGUIGNON et al, 2016) were used as positive controls and amplification of sterile water served as a negative control.…”

“…In Brazil, a high incidence of MRSP in dogs with pyoderma has been reported over the last few years (LOPES et al, 2015;BOTONI et al, 2016;BOURGUIGNON et al, 2016). Moreover, this microorganism has been increasingly implicated in human infections (SOMAYAJI et al, 2016;ROBB et al, 2017).…”

Susceptibilidad antimicrobiana de estafilococos aislados en piodermas de caninos de Coro, Venezuela

“…En el pioderma canino, Staphylococcus intermedius (pseudintermedius) es la bacteria más frecuente ( No es frecuente la prescripción de estudios microbiológicos para el abordaje clínico del pioderma canino (Hensel et al, 2015;Guardabassi et al, 2017), por lo que el diseño de la antibioticoterapia es de carácter empírico, basado en los fármacos con conocida eficacia sobre los estafilococos; empero, se corre el riesgo de utilizar aquellos que favorezcan la resistencia, de allí que la implementación de esta herramienta diagnóstica es recomendable en casos particulares (Cain, 2013;Hensel et al, 2015;Guardabassi et al, 2017). Por otro lado, la respuesta a los tratamientos varía especialmente cuando en el proceso infeccioso están involucradas cepas resistentes a la meticilina como las de Staphylococcus aureus (SARM) y S. pseudintermedius (SPRM), de las cuales ya se tienen reportes de afecciones en animales (Morris et al, 2006; Van Duijkeren et al, 2011; Frank y Loeffler, 2012; Beça et al, 2015), incluyendo dermatitis en caninos con resistencia a penicilina (Antúnez et al, 2009), meticilina (Bourguignon et al, 2016) y hasta multirresistentes (Abusleme, 2009; dos Santos et al, 2016), por lo que la generación de información adecuada a los contextos espacio-temporales sería de utilidad para orientar a los practicantes de la clínica veterinaria en pequeños animales.…”

Infecções estafilocócicas em cães: prevalência, resistência antibacteriana, fatores de risco e de virulência

“…Entretanto, essas bactérias parecem ter um papel oportunista, uma vez que têm sido identificadas em casos de otite externa, piodermites, infecções do trato urinário, assim como o observado neste estudo, além de complicações pós-cirúrgicas[27,29,32].Diversos estudos descrevem as bactérias do gênero Staphylococcus como as mais isoladas da pele e orelhas por constituírem parte da microbiota residente destes locais[31]. O gênero tem sido identificado como o mais importante nos casos de ITUs nos cães[5,29,[39][40][41][42][43][44][45], fato este observado também no presente estudo. em associação ou não ao clavulanato de potássio e a cefalexina são também consideradas drogas de eleição[24].…”