Description of Scheffersomyces henanensis sp. nov., a New D-Xylose-Fermenting Yeast Species Isolated from Rotten Wood

Three strains of a d-xylose-fermenting yeast species were isolated from the host beetle Dorcus titanus collected from two different localities in Henan Province, Central China. These strains formed two hat-shaped ascospores in conjugated and deliquescent asci. Multilocus phylogenetic analysis that included the nearly complete small subunit (SSU), the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rDNAs, as well as RNA polymerase II largest subunit (RPB1) gene demonstrated that these strains represent a novel yeast species belonging to the genus Scheffersomyces. The phylogenetic analysis based on the nucleotide sequences of the xylose reductase (XYL1) gene supported the view that the new strains could be grouped as a unique species. Although this new species is highly similar to Scheffersomyces stipitis-like yeasts in terms of nrDNA sequences and morphological and physiological characteristics, the species can be clearly differentiated from its close relatives on the basis of the sequences of XYL1 and RPB1. Therefore, a novel yeast species, Scheffersomyces titanus sp. nov., is proposed to accommodate these strains. The type strain is NYNU 14712T (CICC 33061T = CBS 13926T).

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“…1). The tree topology is in agreement with topologies obtained previously3567. In the tree obtained with XYL1 gene sequences (Fig.…”

Section: Resultssupporting

confidence: 91%

Taxonomy and physiological characterisation of Scheffersomyces titanus sp. nov., a new D-xylose-fermenting yeast species from China

Liu

Cao

Ren

et al. 2016

Sci Rep

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“…A type strain of S. passalidarum , ATCC MYA-4345 (CBS 10155 = NRRL Y27907), has been isolated from the gut of Odontotaenius disjunctus (wood-boring beetle) in the eastern part of the United States [ 15 ]. Other strains of S. passalidarum have been isolated from rotting wood collected, wood-boring beetles and log galleries sampled in Brazilian Amazonian Forest [ 24 , 25 ] and rotten wood in central China [ 26 ]. S. passalidarum CMUWF1–2, however, was firstly isolated from soil and firstly found in Southeast Asia.…”

Section: Discussionmentioning

confidence: 99%

Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1–2

2018

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BackgroundEfficient bioconversion of lignocellulosic biomass to bioethanol is one of key challenges in the situation of increasing bioethanol demand. The ethanologenic microbes for such conversion are required to possess abilities of utilization of various sugars including xylose and arabinose in lignocellulosic biomass. As required additional characteristics, there are a weak or no glucose repression that allows cells to simultaneously utilize various sugars together with glucose and thermotolerance for fermentation at high temperatures, which has several advantages including reduction of cooling cost. Spathaspora passalidarum ATCC MYA-4345, a type strains, isolated previously have mainly of these abilities or characteristics but its thermotolerance is not so strong and its glucose repression on xylose utilization is revealed.ResultsNewly isolated S. passalidarum CMUWF1–2 was found to have a high ability to produce ethanol from various sugars included in lignocellulosic biomass at high temperatures. The strain achieved ethanol yields of 0.43 g, 0.40 g and 0.20 g ethanol/g xylose at 30 °C, 37 °C and 40 °C, respectively. Interestingly, no significant glucose repression was observed in experiments with mixed sugars, being consistent with the strong resistance to 2-deoxyglucose, and antimycin A showed no effect on its growth in xylose medium. Moreover, the strain was tolerant to glucose and ethanol at concentrations up to 35.0% (w/v) and 8.0% (v/v), respectively.ConclusionsS. passalidarum CMUWF1–2 was shown to achieve efficient production of ethanol from various sugars and a high ethanol yield from xylose with little accumulation of xylitol. The strain also exhibited stress-resistance including thermotolerance and no detectable glucose repression as beneficial characteristics. Therefore, S. passalidarum CMUWF1–2 has remarkable potential for conversion of lignocellulosic biomass to bioethanol.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1218-4) contains supplementary material, which is available to authorized users.

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“…Two isolates were also obtained from rotten wood by Ren et al . (), among 105 yeast strains sampled in central China. More recently, six strains from a total of 792 yeast isolates obtained from wood‐boring beetles and log galleries sampled in Brazilian Amazonian Forest sites were reported (Souza et al ., ).…”

Section: Spathaspora Passalidarum: Candidate For ‘Domestication’ and mentioning

confidence: 99%

The yeasts of the genus Spathaspora: potential candidates for second‐generation biofuel production

Cadete

Rosa

2017

Yeast

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Yeasts of the Spathaspora clade have the ability to convert d-xylose to ethanol and/or xylitol. This is an important trait, as these yeasts may be used to produce bioethanol from lignocellulosic biomass or as a source of new d-xylose metabolism genes for recombinant industrial strains of Saccharomyces cerevisiae. The core group of the genus Spathaspora has 22 species, both formally described and not yet described. Other species, such as Sp. allomyrinae, Candida alai, C. insectamans, C. lyxosophila, C. sake, Sp. boniae and C. subhashii are weakly associated with this clade, based on LSU rRNA gene D1/D2 sequence analyses. Spathaspora passalidarum, Sp. arborariae, Sp. gorwiae and Sp. hagerdaliae produce mostly ethanol from d-xylose, whereas the remaining species within the Spathaspora clade already tested for this property may be considered xylitol producers. Among the d-xylose-fermenting Spathaspora species, Sp. passalidarum is the best ethanol producer, displaying high ethanol yields and productivities when cultured in media supplemented with this pentose under oxygen-limited or anaerobic conditions. The species also exhibits rapid d-xylose consumption and the ability to ferment glucose, xylose and cellobiose simultaneously. These characteristics suggest that Sp. passalidarum is a potential candidate for domestication and use in the fermentation of lignocellulosic materials. Copyright © 2017 John Wiley & Sons, Ltd.

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Description of Scheffersomyces henanensis sp. nov., a New D-Xylose-Fermenting Yeast Species Isolated from Rotten Wood

Cited by 17 publications

References 33 publications

Taxonomy and physiological characterisation of Scheffersomyces titanus sp. nov., a new D-xylose-fermenting yeast species from China

Taxonomy and physiological characterisation of Scheffersomyces titanus sp. nov., a new D-xylose-fermenting yeast species from China

Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1–2

The yeasts of the genus Spathaspora: potential candidates for second‐generation biofuel production

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