Description of the mitogenome and phylogeny of Aedes spp. (Diptera: Culicidae) from the Amazon region

Silva, Lucas Henrique da Silva e; Silva, Fábio Silva da; Medeiros, Daniele Barbosa de Almeida; Cruz, Ana Cecília Ribeiro; Silva, Sandro Patroca da; Aragão, Andressa de Oliveira; Dias, Daniel Damous; Nascimento, Bruna Laís Sena do; Júnior, José Wilson Rosa; Vieira, Durval Bertram Rodrigues; Monteiro, Hamilton Antônio de Oliveira; Neto, Joaquim Pinto Nunes

doi:10.1016/j.actatropica.2022.106500

Cited by 4 publications

(7 citation statements)

References 75 publications

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“…The RSCU value was greater than 1 in all NNA and NNU types. This fixed distribution pattern was similar to those reported in other mosquito species [42].…”

Section: Codon Usage In the Protein-coding Regionssupporting

confidence: 90%

Phylogenetic Analysis of Some Species of the Anopheles hyrcanus Group (Diptera: Culicidae) in China Based on Complete Mitochondrial Genomes

Dong¹,

Yuan

Yang³

et al. 2023

Genes

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Some species of the Hyrcanus group are vectors of malaria in China. However, the member species are difficult to identify accurately by morphology. The development of sequencing technologies offers the possibility of further studies based on the complete mitochondrial genome. In this study, samples of mosquitoes of the Hyrcanus group were collected in China between 1997 and 2015. The mitochondrial genomes of ten species of the Hyrcanus group were analyzed, including the structure and base composition, codon usage, secondary structure of tRNA, and base difference sites in protein coding regions. Phylogenetic analyses using maximum-likelihood and Bayesian inference were performed based on mitochondrial genes and complete mitochondrial genomes The mitochondrial genome of 10 Hyrcanus group members ranged from 15,403 bp to 15,475 bp, with an average 78.23% (A + T) content, comprising of 13 PCGs (protein coding genes), 22 tRNAs, and 2 rRNAs. Site differences between some closely related species in the PCGs were small. There were only 36 variable sites between Anopheles sinensis and Anopheles belenrae for a variation ratio of 0.32% in all PCGs. The pairwise interspecies distance based on 13 PCGs was low, with an average of 0.04. A phylogenetic tree constructed with the 13 PCGs was consistent with the known evolutionary relationships. Some phylogenetic trees constructed by single coding regions (such as COI or ND4) or combined coding regions (COI + ND2 + ND4 + ND5 or ND2 + ND4) were consistent with the phylogenetic tree constructed using the 13 PCGs. The phylogenetic trees constructed using some coding genes (COII, ND5, tRNAs, 12S rRNA, and 16S rRNA) differed from the phylogenetic tree constructed using PCGs. The difference in mitochondrial genome sequences between An. sinensis and An. belenrae was very small, corresponding to intraspecies difference. The combination of all 13 PCG sequences was demonstrated to be optimal for phylogenetic analysis in closely related species.

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“…The RSCU value was greater than 1 in all NNA and NNU types. This fixed distribution pattern was similar to those reported in other mosquito species [42].…”

Section: Codon Usage In the Protein-coding Regionssupporting

confidence: 90%

Phylogenetic Analysis of Some Species of the Anopheles hyrcanus Group (Diptera: Culicidae) in China Based on Complete Mitochondrial Genomes

Dong¹,

Yuan

Yang³

et al. 2023

Genes

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“…aegypti specimen sampled from one of the populations found in the municipality of Balsas, in the Brazilian state of Maranhão. The sequence obtained is subdivided into 37 functional subunits, in addition to a partial portion of the control region rich in adenine and thymine (A+T), with the characteristic arrangement and disposition of the genes along the two strands of transcription, as observed in all the previous description of the mitochondrial genomes of mosquitoes, in particular, those of the tribe Aedini [20,[22][23][24]58]. The nucleotide sequence obtained here share a high level of identity with a reference genome (GenBank ID: NC_035159), which was obtained in a study that also described the three chromosomes of the species [58].…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the mitochondrial (mtDNA) genome is an important tool for evolutionary studies, given its uniparental inheritance, lack of recombination and high mutation rates, in comparison with the nuclear genome [13][14][15][16]. Mitochondrial markers are especially useful for the study of vectors of medical and epidemiological importance [17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Sequencing and Analysis of the Mitochondrial Genome of Aedes aegypti (Diptera: Culicidae) from the Brazilian Amazon Region

Sousa,

Cruz,

Silva

et al. 2023

Insects

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Aedes aegypti is a mosquito native to the African continent, which is now widespread in the tropical and subtropical regions of the world. In many regions, it represents a major challenge to public health, given its role in the cycle of transmission of important arboviruses, such as Dengue, Zika, and Chikungunya. Considering the epidemiological importance of Ae. aegypti, the present study sequenced the partial mitochondrial genome of a sample collected in the municipality of Balsas, in the Brazilian state of Maranhão, followed by High Throughput Sequencing and phylogenetic analyses. The mitochondrial sequence obtained here was 15,863 bp long, and contained 37 functional subunits (thirteen PCGs, twenty-two tRNAs and two rRNAs) in addition to a partial final portion rich in A+T. The data obtained here contribute to the enrichment of our knowledge of the taxonomy and evolutionary biology of this prominent disease vector. These findings represent an important advancement in the understanding of the characteristics of the populations of northeastern Brazil and provide valuable insights into the taxonomy and evolutionary biology of this prominent disease vector.

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“…Following the method outlined by Rakotonirina et al 21 , DNA was individually extracted from each mosquito's body using a DNA Blood and Tissue Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer's guidelines. The extracted DNA underwent amplification via polymerase chain reaction (PCR) utilizing specific primers targeting mitochondrial genes which are widely used markers in evolutionary and taxonomic studies due to the lack of DNA recombination, simplifying inheritance patterns, in contrary to the nuclear gene 27 . We focused on cytochrome c oxidase subunits 1, 2, and 3 ( cox 1, cox 2, and cox 3) and cytochrome b, all known to be under strong purifying selection in mosquitoes 27 , 28 .…”

Section: Methodsmentioning

confidence: 99%

“…The extracted DNA underwent amplification via polymerase chain reaction (PCR) utilizing specific primers targeting mitochondrial genes which are widely used markers in evolutionary and taxonomic studies due to the lack of DNA recombination, simplifying inheritance patterns, in contrary to the nuclear gene 27 . We focused on cytochrome c oxidase subunits 1, 2, and 3 ( cox 1, cox 2, and cox 3) and cytochrome b, all known to be under strong purifying selection in mosquitoes 27 , 28 . However, during our study, technical limitations resulted in interpretable data only for cox 1 and cox 3.…”

Section: Methodsmentioning

confidence: 99%

Speciation patterns of Aedes mosquitoes in the Scutellaris Group: a mitochondrial perspective

Rakotonirina,

Dauga,

Pol

et al. 2024

Sci Rep

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The Scutellaris Group of Aedes comprises 47 mosquito species, including Aedes albopictus. While Ae. albopictus is widely distributed, the other species are mostly found in the Asia–Pacific region. Evolutionary history researches of Aedes species within the Scutellaris Group have mainly focused on Ae. albopictus, a species that raises significant public health concerns, neglecting the other species. In this study, we aimed to assess genetic diversity and estimate speciation times of several species within the Scutellaris Group. Mosquitoes were therefore collected from various Asia–Pacific countries. Their mitochondrial cytochrome c oxidase subunit 1 (cox1) and subunit 3 (cox3) sequences were analyzed alongside those of other Scutellaris Group species available in the GenBank database. To estimate the divergence time, we analyzed 1849 cox1 gene sequences from 21 species, using three species (Aedes aegypti, Aedes notoscriptus and Aedes vigilax) as outgroups. We found that most of the speciation dates occurred during the Paleogene and the Neogene periods. A separation between the Scutellaris Subgroup and the Albopictus Subgroup occurred approximately 64–61 million years ago (MYA). We also identified a split between species found in Asia/Micronesia and those collected in Melanesia/Polynesia approximately 36–35 MYA. Our findings suggest that the speciation of Aedes species within the Scutellaris Group may be driven by diversity in mammalian hosts, climate and environmental changes, and geological dynamics rather than human migration.

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Description of the mitogenome and phylogeny of Aedes spp. (Diptera: Culicidae) from the Amazon region

Cited by 4 publications

References 75 publications

Phylogenetic Analysis of Some Species of the Anopheles hyrcanus Group (Diptera: Culicidae) in China Based on Complete Mitochondrial Genomes

Phylogenetic Analysis of Some Species of the Anopheles hyrcanus Group (Diptera: Culicidae) in China Based on Complete Mitochondrial Genomes

Sequencing and Analysis of the Mitochondrial Genome of Aedes aegypti (Diptera: Culicidae) from the Brazilian Amazon Region

Speciation patterns of Aedes mosquitoes in the Scutellaris Group: a mitochondrial perspective

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