Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

Abstract

Three previously unidentified Gram-positive anaerobic coccoid bacteria, strains KhD-2 , KHD4 , and Kh-D5 , isolated from a vaginal swab, were characterized using the taxonogenomics concept. The phylogenic analysis, phenotypic characteristics, and genotypic data presented in this report attest that these three bacteria are distinct from previously known bacterial species with standing in nomenclature and represent three new Peptoniphilus species. Strain KhD-2 is most closely related to Peptoniphilus sp. DNF00840 and Peptoniphilus harei (99.7% and 98.2% identity, respectively); strain KHD4 to Peptoniphilus lacrimalis (96%) and strain Kh-D5 to Peptoniphilus coxii (97.2%). Strains KhD-2 , KHD4 , and Kh-D5 DNA G+C contents are, respectively, 34.23%, 31.87%, and 49.38%; their major fatty acid was C (41.6%, 32.0%, and 36.4%, respectively). We propose that strains KhD-2 (=CSUR P0125 = DSM 101742), KHD4 (=CSUR P0110 = CECT 9308), and Kh-D5 (=CSUR P2271 = DSM 101839) be the type strains of the new species for which the names Peptoniphilus vaginalis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilu pacaensis sp. nov., are proposed, respectively.

Depiction of Vaginal Microbiota in Women With High-Risk Human Papillomavirus Infection

“…G. vaginalis was also among the six most prevalent proinflammatory taxa identified in the abovementioned South African study ( 33 ). Peptoniphilus and Anaerococcus , two newly identified members of the Peptoniphilaceae family, are significantly associated with BV ( 38 ). Similar to Peptoniphilus and Anaerococcus, Peptostreptococcus (Peptostreptococcaceae) is a common Gram-positive anaerobe that has not been reported to adhere to vaginal epithelial cells, but does usually adhere to epithelial cells at other body sites ( 39 ).…”

Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study

“…Obligate anaerobes are necessarily more difficult to study in the laboratory and their diagnostic cultivation is not routine; it is therefore perhaps not surprising that there is much less knowledge about the role of obligate anaerobes in the vaginal microbiome. New species are continuing to be identified and the roles of anaerobes and their fermentation products in vaginal microbial ecology are being explored, but more work is needed.…”

Lactimicrobium massiliense gen. nov., sp. nov.; Anaerolactibacter massiliensis gen. nov., sp. nov.; Galactobacillus timonensis gen. nov., sp. nov. and Acidipropionibacterium timonense sp. nov. isolated from breast milk from healthy breastfeeding African women

“…Biochemical analysis using various strips (API ® ZYM, API ® 20 A, API ® 50 CH and API Rapid ID 32 A) (bioMérieux) and oxidase and catalase tests (bioMérieux) were done according to the manufacturer's instructions. Analyses were performed as previously described [22]. Motility assay, Gram-staining, transmission electron microscopy and sporulation assay were also performed as describe elsewhere [23].…”

“…Genome sequencing, assemblage, annotation and comparison were performed as previously described [22], [23], [25]. The genomes of Solobacterium moorei strain RCA59-74 T (NZ_AUKY00000000) [19], Bulleidia extructa strain W 1219 T (NZ_ADFR00000000) [26], Anaerorhabdus furcosa strain VPI 3253 T (NZ_FUWY00000000) [27], Holdemania filiformis strain J1-31B-1 T (NZ_ACCF01000000) [28] and Holdemania massiliensis strain AP2 T (CALK01000000) [29] were used for genome comparison of the strains Marseille-P4301, Marseille-P4302 and Marseille-P4641.…”