Description of two cryptic species of the Amolops ricketti group (Anura, Ranidae) from southeastern China

Lyu, Zhi-Tong; Huang, Liusheng; Wang, Jian; Li, Yuanqiu; Chen, Honghui; Qi, Shuo; Wang, Ying-Yong

doi:10.3897/zookeys.812.29956

Cited by 21 publications

(18 citation statements)

References 24 publications

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“…Cryptic species, which are morphologically identical but genetically differentiated, are also common in most species complexes (e.g. Yan et al 2011;Kuraishi et al 2013;Xiong et al 2015;Lyu et al 2019Lyu et al , 2020. With remarkable morphological diversity, but relatively smaller genetic differentiation, R. longicrus, R. zhenhaiensis, and R. culaiensis show a special situation.…”

Section: Discussionmentioning

confidence: 99%

A new species of the Rana japonica group (Anura, Ranidae, Rana) from China, with a taxonomic proposal for the R. johnsi group

Wan¹,

Lyu²,

Qi³

et al. 2020

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Rana jiulingensissp. nov., a new species from Hunan and Jiangxi, southeastern China, is described. The new species is assigned to the R. japonica group. The clade comprising R. jiulingensissp. nov. and R. dabieshanensis from Anhui is the sister taxon of R. omeimontis from Sichuan. Rana jiulingensissp. nov. can be distinguished by the significant divergences in the 16S and COI genes, and the combination of following morphological characters: body size medium, SVL 48.3–57.8 mm in adult males and 48.2–57.5 mm in adult females; dorsolateral fold straight; digits without circummarginal grooves; dorsal skin smooth; tibio-tarsal articulation reaching forward beyond the tip of snout; heels overlapping; webbing formula of toes: I 1⅓ – 2 II 1⅓ – 2⅓ III 1½ – 2⅔ IV 3 – 1⅓ V; absence of vocal sacs in males; and presence of creamy white nuptial pad with tiny hoar spines on the finger I and reddish tubercles on loreal and temporal regions in breeding males. Furthermore, based on our results and the previous literature, R. zhengi is synonymized with R. sangzhiensis, and a new species group, the Rana johnsi group, is proposed for the R. johnsi and R. sangzhiensis. Currently, the Rana contains 41 recognized species, and the phylogenetic placements of several species remain unresolved.

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Section: Discussionmentioning

confidence: 99%

A new species of the Rana japonica group (Anura, Ranidae, Rana) from China, with a taxonomic proposal for the R. johnsi group

Wan¹,

Lyu²,

Qi³

et al. 2020

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“…Genomic DNA were extracted from muscle tissue samples, using DNA extraction kit from Tiangen Biotech (Beijing) Co., Ltd. Two mitochondrion genes, namely partial 16S ribosomal RNA gene (16S) and partial cytochrome C oxidase 1 gene (CO1), were amplified. Primers used for 16S were L3975 (5’-CGCCTGTTTACCAAAAACAT-3’) and H4551 (5’-CCGGTCTGAACTCAGATCACGT-3’), and L2A (5’-CCAAACGAGCCTAGTGATAGCTGGTT-3’) and H10 (5’-TGATTACGCTACCTTTGCACGGT-3’), and for CO1 were Chmf4 (5’-TYTCWACWAAYCAYAAAGAYATCGG-3’) and Chmr4 (5’-ACYTCRGGRTGRCCRAARAATCA-3’), and dgLCO (5’-GGTCAACAAATCATAAAGAYATYGG-3’) and dgHCO (5’-AAACTTCAGGGTGACCAAARAAYCA-3’), following Lyu et al (2019). PCR amplifications were processed with the cycling conditions that initial denaturing step at 95 °C for 4 min, 35 cycles of denaturing at 94 °C for 40 s, annealing at 53 °C (for 16S) / 48 °C (for CO1) for 40 s and extending at 72 °C for 60 s, and a final extending step at 72 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Description of a new species of Music frogs (Anura, Ranidae, Nidirana) from Mt Dayao, southern China

Lyu¹,

Mo²,

Wan³

et al. 2019

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A new species of Music frogs, Nidiranayaoicasp. nov. is described based on a series of adult male specimens collected from Mt Dayao, Guangxi, southern China, providing valuable new information on the phylogeny, bioacoustics, and biogeography of related species within the genus Nidirana. The new species forms the sister taxon to N.daunchina from western China and together the sister taxon to N.chapaensis from northern Vietnam. Nidiranayaoicasp. nov. can be distinguished from all known congeners by a significant genetic divergence in the mitochondrial 16S and CO1 genes, the advertisement call containing 1–3 rapidly repeated regular notes, and the combination of morphological characteristics including a medium-sized body with SVL 40.4–45.9 mm in adult males; lateroventral grooves on every digit, not meeting at the tip of disk; tibio-tarsal articulation reaching the nostril; the presence of a pair of subgular vocal sacs in males; and one single developed nuptial pad on dorsal surface of first finger in males.

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“…For instance, while breeding males of the common frog Rana temporaria present baggy skin (Ao et al, 2006), this species has not been considered capable of vocalizing underwater; however, a recording of such calls of two males can be located in the FonoZoo collection at http://www.fonozoo.com/ fnz_detalles_registro_amphibia.php?xml:id=68770&tipo_reg istro=1#modal. Compared to A. ricketti, its sister species A. wuyiensis has been reported to produce aerial calls with similar repetition rates and dominant frequencies but more notes (Zhang, Chen & Zhao, 2013;Pyron, 2014;Lyu et al, 2019). However, the observation of loose skin in male A. wuyiensis (Fei et al, 2009a) and A. yatseni, a close relative (see Fig.…”

Section: Discussionmentioning

confidence: 98%

“…However, the observation of loose skin in male A. wuyiensis (Fei et al ., ) and A. yatseni , a close relative (see Fig. 6B in Lyu et al ., ), suggests a potential focus for field observations of these frogs: underwater metabolically costly behaviors including vocalization.…”

Section: Discussionmentioning

confidence: 99%

“…To determine whether the loose skin of the flanks and thighs is a male nuptial trait in A. ricketti, eleven specimens deposited at the Chengdu Institute of Biology (CIB) from three localities were examined: the study site, CIB-IOZCAS 10368; Kuatun (type locality), N27°44 0 , E117°38 0 , CIB-IOZCAS 09235, 09238, 09243; and Jinggangshan, N26°31 0 , E114°06 0 , CIB-IOZCAS 09318-09320, 09332, 09390-09392. Given the active taxonomic revisions related to A. ricketti in recent years, a~550 bp fragment of the mitochondrial 16S gene was obtained for seven representatives of these individuals and was compared with 50 published sequences from A. ricketti (GenBank accession numbers MK263244, MK263246, MK263256, MK263259-MK263261), its sister species Amolops wuyiensis (MK263239, MK263240, MK263245, MK263257, MK263258), and closely related species Amolops albispinus, Amolops sinensis, Amolops yatseni and Amolops yunkaiensis (MK263247-MK263255, MK263262, MK263265, MK263269-MK263280, MK263287-MK263302) (Pyron, 2014;Sung et al, 2016;Lyu et al, 2018Lyu et al, , 2019. The primers LR-N-13398 (5 0 -CgCCTgTTTA CCAAAAACAT-3 0 ) and LR-J-12887 (5 0 -CCggTCTgAA CTCAgATCAC gT-3 0 ) from Simon et al (1994) were used for PCR and Sanger sequencing.…”

Section: Methodsmentioning

confidence: 99%

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The co‐occurrence of loose skin and underwater calling in frogs—further evidence from Amolops ricketti and its implications

2019

Journal of Zoology

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Underwater vocalizations of frogs are usually inaudible to the human ear and have been poorly documented. Some authors believe that they are more common than currently recognized, yet there have been no new records at the genus level for nearly 30 years. I report on the recordings of underwater calling by the ranid species Amolops ricketti (Boulenger, 1899) from a mountain stream in southern China. Males emitted 2.2–3.5 calls per minute under submerged rocks, which were inaudible to the human ear. Most of the recorded calls lasted 0.73–2.09 s and contained a weak initial note with fewer pulses (1–5) and one to three subsequent notes (3–13 pulses each) repeated at a rate of 0.82–1.99 notes·s−1, with an internote interval lasting 0.36–1.19 s. The dominant frequency of calls ranged from 2.1 to 3.0 kHz, and the notes exhibited increasing intensity and slight upward frequency modulation. Comparisons between individuals of different sexes and breeding stages reveal that the loose skin of the flanks and thighs is a male nuptial trait in A. ricketti. The finding extends the phylogenetic distribution of underwater vocalization to a distinct lineage and constitutes another instance of loose‐skin frogs calling underwater, which can also been identified in Pelobates (Pelobatidae), Leptobrachium (Megophryidae), Telmatobius (Telmatobiidae), Rana italica and Rana sakuraii (Ranidae). These independent cases may allow the proposal of an evolutionary correlation of the two traits in anurans, as a large surface/volume ratio facilitates high levels of cutaneous respiration required for the effective performance of underwater calling. An awareness of this possibility may aid in increasing our understanding of the natural history of anurans.

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Description of two cryptic species of the Amolops ricketti group (Anura, Ranidae) from southeastern China

Cited by 21 publications

References 24 publications

A new species of the Rana japonica group (Anura, Ranidae, Rana) from China, with a taxonomic proposal for the R. johnsi group

A new species of the Rana japonica group (Anura, Ranidae, Rana) from China, with a taxonomic proposal for the R. johnsi group

Description of a new species of Music frogs (Anura, Ranidae, Nidirana) from Mt Dayao, southern China

The co‐occurrence of loose skin and underwater calling in frogs—further evidence from Amolops ricketti and its implications

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