Description of two new species of Nostoc from China based on the polyphasic approach

Cai, Fangfang; Yu, Ge; Liu, Yuchen; Sun, Yuanquan; Li, Renhui

doi:10.5507/fot.2021.011

Cited by 7 publications

(6 citation statements)

References 54 publications

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“…These structures may be unique characteristics of the genus Amazonocrinis and can be used to confirm the correction of ITS sequence data. The ITS secondary folding patterns of A. thailandica were significantly different from other Amazonocrinis taxa and closely related taxa within Nostocaceae reported in previous studies (Osorio-Santos et al 2014, Mai et al 2018, Alvarenga et al 2021, Cai et al 2021, Pietrasiak et al 2021, supporting the establishment of this novel Amazonocrinis species. A. thailandica showed distinctly different structures of the V2 and V3 helices from all other Amazonocrinis taxa, highlighting the novelty of our species and supporting the phylogenetic analyses (Fig.…”

supporting

confidence: 47%

“…Additional molecular data from the utilization of ITS secondary structures together with the 16S rRNA gene sequences have been advocated as essential for the recognition of cyanobacterial species (Osorio-Santos et al 2014, Mai et al 2018, Cai et al 2021, Pietrasiak et al 2021. In particular, the D1-D1′, Box-B, V2, and V3 helices in…”

Section: Characterization Of Its Secondary Structuresmentioning

confidence: 99%

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Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Tawong¹,

Pongcharoen²,

Pongpadung³

et al. 2022

Algae

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Cyanobacteria are distributed worldwide, and many new cyanobacterial species are discovered in tropical region. TheNostoc-like genus Amazonocrinis has been separated from the genus Nostoc based on polyphasic methods. However,species diversity within this genus remains poorly understood systematically because only one species (Amazonocrinisnigriterrae) has been described. In this study, two novel strains (NUACC02 and NUACC03) were isolated from moist ricefield soil in Thailand. These two strains were characterized using a polyphasic approach, based on morphology, 16S rRNAphylogenetic analysis, internal transcribed spacer secondary structure and ecology. Phylogenetic analyses based on 16SrRNA gene sequences confirmed that the two novel strains formed a monophyletic clade related to the genus Amazonocrinisand were distant from the type species A. nigriterrae. The 16S rRNA gene sequence similarity (<98.1%) betweennovel strains and all other closely related taxa including the Amazonocrinis members exceeded the cutoff for speciesdelimitation in bacteriology, reinforcing the presence of a new Amazonocrinis species. Furthermore, the novel strainspossessed unique phenotypic characteristics such as the presence of the sheath, necridia-like cells, larger cell dimensionand akinete cell arrangement in long-chains and the singularity of D1–D1′, Box-B, V2, and V3 secondary structures thatdistinguished them from other Amazonocrinis members. Considering all the results, we described our two strains as Amazonocrinisthailandica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.

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supporting

confidence: 47%

Section: Characterization Of Its Secondary Structuresmentioning

confidence: 99%

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Tawong¹,

Pongcharoen²,

Pongpadung³

et al. 2022

Algae

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“…Analysis of the 16S-23S ITS secondary structure has been used as an effective tool to distinguish taxa at the species level [6,19,[58][59][60][61][62]. The analyses of the secondary structures of the ITS further supported the distinctiveness of N. hunanesis.…”

Section: Discussionmentioning

confidence: 95%

Nodosilinea hunanesis sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach

Cai

Zhang

et al. 2022

Diversity

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A cyanobacterial strain (ZJJ01), morphologically identified as a Leptolyngbya-like species was isolated from a freshwater pond in Zhangjiajie National Forest Park, China, and it was characterized through a polyphasic study based on morphological, ecological, and molecular data. Morphologically, the strain fits the description of Leptolyngbya well, but under further examination, it formed a distinctive structure, namely nodules, which confirmed that it belonged to the genus of Nodosilinea. The 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses clearly confirmed that the studied strain belongs to the genus Nodosilinea but that it is phylogenetically distinct from the 10 other species of Nodosilinea. Furthermore, The D1–D1’ and Box–B helix of the 16S–23S ITS region of the strain ZJJ01 were also different from those of previously described Nodosilinea species. On the basis of this polyphasic approach, here, we provide a description of the new taxon: Nodosilinea hunanesis sp. nov.

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“…Furthermore, M. dolomitestris and M. aguilerae are 17.91-17.95% dissimilar from each other. According to several studies, if strains in the same genus are >7% dissimilar in the ITS region, it is considered strong evidence that they belong to separate species (Erwin and Thacker 2008, Osorio-Santos et al 2014, Gonz alez-Resendiz et al 2018a, 2018b, Mai et al 2018, V azquez-Mart ınez et al 2018, Mare s et al 2019, Jung et al 2020, Cai et al 2021, Hauerov a et al 2021, Mikhailyuk et al 2022. The marked dissimilarity among these strains is considerably higher than this threshold, and on this basis alone, the three species are justified.…”

Section: Mojavia Pulchramentioning

confidence: 98%

Mojavia aguilerae and M. dolomitestris — two new Nostocaceae (Cyanobacteria) species from the Americas

Baldarelli

Pietrasiak

Osorio‐Santos

et al. 2022

Journal of Phycology

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While nostocacean cyanobacteria are ubiquitous and play critical roles in terrestrial ecosystems, their taxonomy and biogeography still entail mysteries. We isolated two Nostoc‐like cyanobacteria from biological soil crusts of the Atacama (Chile) and Mojave (USA) Deserts. An initial 16S rRNA gene phylogeny placed both in monophyly with Mojavia pulchra. Here, we describe two new species of the previously monotypic Mojavia using a polyphasic approach including morphology, 16S rRNA phylogenies, secondary structure, and percent similarity of the 16S‐23S ITS region. Like M. pulchra, both new species produce compact microcolonies, arthrospore‐like akinetes, and monocytes, traits characteristic of the genus. Mojavia aguilerae sp. nov. is morphologically distinct from both other species in producing bluntly conical end cells, abundant enlarged akinetes in multiseriate filaments, and gold‐colored cells during senescence. Mojavia dolomitestris sp. nov. exhibited distinctly firm, light‐colored, compartmentalized mucilage. M. dolomitestris is somewhat cryptic with M. pulchra, but has more densely packed microcolonies, rarity and later onset of brownish sheath pigmentation, and an origin from soils derived from dolomite. The two new species strengthened the position of Mojavia as a robust genus sister to Nostoc. Although 16S rRNA gene data could not separate the Mojavia species from each other, the three species showed distinct dissimilarities in secondary ITS structure and differed greatly from Nostoc sensu stricto. The high dissimilarities between their 16S‐23S ITS regions suggest a long evolutionary history of the three species as separate lineages. Mojavia is an evolutionary and ecologically unique nostocacean genus, and its rarity and restricted habitat point to an urgent need for recognition and protection.

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Description of two new species of Nostoc from China based on the polyphasic approach

Cited by 7 publications

References 54 publications

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

Nodosilinea hunanesis sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach

Mojavia aguilerae and M. dolomitestris — two new Nostocaceae (Cyanobacteria) species from the Americas

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