Descriptions of four new species of Bactrocera and new country records highlight the high biodiversity of fruit flies in Vietnam (Diptera, Tephritidae, Dacinae)

Leblanc, Luc; Doorenweerd, Camiel; Jose, Michael San; Pham, Hong Thai; Rubinoff, Daniel

doi:10.3897/zookeys.797.29138

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…Bactrocera syzygii White & Tsuruta new country record: Formerly known only from a small series of specimens bred from rose-apple ( Syzygium jambos ) in Sri Lanka (Drew and Romig 2013), it was recently recorded from India (David et al 2017). The use of zingerone-baited traps collected large numbers in Sri Lanka (Leblanc et al 2018b), Bangladesh (Table 1), and as far east as Vietnam (Leblanc et al 2018a) and south as Sarawak and Sulawesi (CD, unpublished), consistent with the widespread distribution of rose-apple.…”

Section: Resultsmentioning

confidence: 65%

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Six years of fruit fly surveys in Bangladesh: a new species, 33 new country records and discovery of the highly invasive Bactrocera carambolae (Diptera, Tephritidae)

Leblanc

Hossain

Doorenweerd

et al. 2019

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We engaged in six years of snap-shot surveys for fruit flies in rural environments and ten protected forest areas of Bangladesh, using traps baited with male lures (cue-lure, methyl eugenol, zingerone). Our work has increased the recorded number of species of Tephritidae in the country from seven to 37. We summarize these surveys and report eight new country occurrence records, and a new species (Zeugodacus madhupuri Leblanc & Doorenweerd, sp. nov.) is described. The highlight among the new records is the discovery, and significant westward range extension, of Bactrocera carambolae Drew & Hancock, a major fruit pest detected in the Chattogram and Sylhet Divisions. We rectify the previously published erroneous record of Bactrocera bogorensis (Hardy), which was based on a misidentification of Zeugodacus diaphorus (Hendel). We also report the occurrence in Bangladesh of nine other Tephritidae, the rearing of three primary fruit fly parasitoids from Zeugodacus, and records of non-target attraction to fruit fly lures.

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Section: Resultsmentioning

confidence: 65%

“…A non-pest species bred from Symplocos cochinchinensis , S. racemosa (Symplocaceae), and Gmelina arborea (Verbenaceae) (Allwood et al 1999). A closely related species with entirely fulvous femora, B. clarifemur Leblanc & Doorenweerd, was recently described from Vietnam (Leblanc et al 2018a). All Bangladesh specimens belong to B. pendleburyi .…”

Section: Resultsmentioning

confidence: 99%

Six years of fruit fly surveys in Bangladesh: a new species, 33 new country records and discovery of the highly invasive Bactrocera carambolae (Diptera, Tephritidae)

Leblanc

Hossain

Doorenweerd

et al. 2019

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“…The COI-5P section is the ‘DNA barcode’ segment (Hebert et al 2003a), and is prevalent in the Barcode of Life reference database ‘BOLD’ (Ratnasingham and Hebert 2007). The COI-3P segment has been used as one of seven markers in a molecular phylogeny of Dacini (San Jose et al 2018a), as well as to support species delimitation (Leblanc et al 2018, 2019) and population source exclusion of B. dorsalis (Barr et al 2014). The COI-FF segment has a forward primer inside the COI-5P fragment to avoid a nuclear pseudogene in the Queensland fruit fly, Bactrocera tryoni Frogatt, and is adopted as the standard diagnostic fragment in the Australian Handbook for the Identification of Fruit Flies (Blacket et al 2012; Schutze et al 2018).…”

Section: Methodsmentioning

confidence: 99%

DNA barcodes and reliable molecular identifications in a diverse group of invasive pests: lessons fromBactrocerafruit flies on variation across the COI gene, introgression, and standardization

Doorenweerd

Leblanc

et al. 2020

Preprint

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The utility of a universal DNA ‘barcode’ fragment of 658 base pairs of the Cytochrome C Oxidase I (COI) gene for the recognition of all animal species has been a widely debated topic on theoretical and practical levels. Regardless of its challenges, large amounts of COI sequence data have been produced in the last two decades. To optimally use the data towards reliable species identification will require further steps to validate the method and reference libraries. The fruit fly tribe Dacini holds about a thousand species, of which eighty are pests of economic concern, including some of the world’s foremost fruit and vegetable pests, and there are many morphologically cryptic species complexes in the tribe. Where previous studies showed limited success in using COI to identify Dacini, our results with a highly curated morphological dataset indicate high congruence between morphology and COI: 90% of the species in our 5,576 sequences, 262-species global dataset can be identified with COI alone based on a monophyly criterion. However, in some key pest species belonging to complexes that were previously thought diagnosable with COI, we found that expanded sampling and independent validation of identifications using genomic data revealed introgression of mitochondrial DNA. We find that the informative SNPs are uniformly distributed across the COI gene, and we provide recommendations for standardization. We conclude that reliable molecular identifications with COI require extensive species coverage, population sampling, and genomics-supported reference identifications before they can be validated as a “diagnostic” marker for specific groups.

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“…Natural populations of B . dorsalis exhibit extreme variation in adult pigment patterns 29,51 . This intraspecific diversity is particularly noticeable in their thorax (scutum), which ranges from pale reddish-brown to black, often with lanceolate-patterned intermediates.…”

Section: Discussionmentioning

confidence: 99%

The genetic basis of the black pupae phenotype in tephritid fruit flies

Paulo,

Nguyen,

Ward

et al. 2024

Preprint

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The remarkable diversity of insect pigmentation offers a captivating avenue for exploring evolution and genetics. In tephritid fruit flies, decoding the molecular pathways underlying pigmentation traits also plays a central role in applied entomology. Mutant phenotypes like the black pupae (bp) have long been used as a component of genetic sexing strains, allowing male-only release in tephritid sterile insect technique applications. However, the genetic basis of bp remains largely unknown. Here, we present independent evidence from classical and modern genetics showing that the bp phenotype in the GUA10 strain of the Mexican fruit fly,Anastrepha ludens, is caused by a large deletion at theebonylocus resulting in the removal of the entire protein-coding region of the gene. Targeted knockout ofebonyinduced analogous bp phenotypes across six tephritid species spanning over 50 million years of divergent evolution. This functionally validated our findings and allowed for a deeper investigation into the role of Ebony in pigmentation and development in these species. Our study offers fundamental knowledge for developing new sexing strains based on the bp marker and for future evolutionary developmental biology studies in tephritid fruit flies.

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Descriptions of four new species of Bactrocera and new country records highlight the high biodiversity of fruit flies in Vietnam (Diptera, Tephritidae, Dacinae)

Cited by 9 publications

References 25 publications

Six years of fruit fly surveys in Bangladesh: a new species, 33 new country records and discovery of the highly invasive Bactrocera carambolae (Diptera, Tephritidae)

Six years of fruit fly surveys in Bangladesh: a new species, 33 new country records and discovery of the highly invasive Bactrocera carambolae (Diptera, Tephritidae)

DNA barcodes and reliable molecular identifications in a diverse group of invasive pests: lessons fromBactrocerafruit flies on variation across the COI gene, introgression, and standardization

The genetic basis of the black pupae phenotype in tephritid fruit flies

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