Descriptive analysis, DNA barcoding and condition index of Penaeids (Crustacea: Decapoda) from the Egyptian Mediterranean coast

Sharawy, Zaki Z.; Abbas, Eman M.; Khafage, Amal R.; Galal-Khallaf, Asmaa; Ismail, Rania F.; Ahmed, Hamdy O.; Mohammed-Geba, Khaled; Kato, Mikio

doi:10.1016/j.fishres.2016.12.007

Cited by 12 publications

(13 citation statements)

References 34 publications

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“…In Egypt, the application of such taxonomic tool is still in an early stage. For example, it was used for characterization of different shrimp species in the Mediterranean and Red Seas (Sharawy et al., 2017; Abbas et al., 2018), as well as identification of commercial alteration in fisheries-related products (Galal-Khallaf et al., 2014). Genetic and morphological characterization of aquatic resources in Egypt can provide tools of great importance for aquaculture development.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular clues for recording the first appearance of Artemia franciscana ( ) in Egypt

Sheir

Galal-Khallaf

Mohamed

et al. 2018

Heliyon

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Artemia franciscana is a native species to the New World, and became an exotic species to most parts of the world. The Egyptian hypersaline, continental Qaroun Lake (Fayoum Governorate, Middle of Egypt) is subjected to a gradually increasing salinity rates that approximate or exceed these of seawater. Artemia populations there are known to be parthenogenetic. Yet, these populations started to exhibit abnormal morphologies. Therefore, Qaroun Lake samples of Artemia were subjected to several morphological, biometric, and molecular phylogenetic analyses for accurate species identification and phylogeographic origin approximation. These analyses revealed the existence of the alien sexual species of brine shrimp A. franciscana in Qaroun Lake. The characteristics of the subspherical frontal knob with several spines on the top, ovisac lateral triangular lobe on both sides and its projection together with the biometrics confirmed this species morphotype. DNA barcoding and other molecular analyses based on PCR-based amplification and sequencing of the barcode region of the cytochrome oxidase subunit I gene (COI) exhibited that all the collected samples belong to five haplotypes. Egyptian A. franciscana COI sequences phylogeny and pairwise distances analysis exhibited closer proximity to Latin American strains than to the Northern American ones. A. franciscana presence may be ascribed to the migratory birds present in Qaroun Lake protectorate, since no marine aquaculture activity in Qaroun Lake is known. Therefore, and for the best of our knowledge, this is the first record of the invasive A. franciscana in Egypt.

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Section: Introductionmentioning

confidence: 99%

Morphological and molecular clues for recording the first appearance of Artemia franciscana ( ) in Egypt

Sheir

Galal-Khallaf

Mohamed

et al. 2018

Heliyon

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“…Therefore, this study, to the best of our knowledge, is the first to provide both morphological and genetic markers for identification of M. latisulcatus, and also for identification of the genus Melicertusin the world. It is worth to mention that, not only COI was proven as a good marker for discrimination of crustacean species (Sharawy et al, 2017) butalso 16SrRNA gene has been suggested as abetter marker especially for higher crustacean species. Therefore, we used both markers; in order to provide adequate molecular clues for M. latisulcatus identification.…”

Section: Discussionmentioning

confidence: 99%

“…To avoid bias due to different measuring tools, or due to human errors, and seeking more accuracy (Sharawy et al, 2017), individual lengths were measured to the nearest 0.1 cm applying a Matlab routine (The Math works, Inc., Natick, MA) on the individual prawn snapshot that has been taken using a USB camera (Leica MEGA 0.I.S) equipped with a DC VARIO-ELMARIT 1:2.8-4916.3-25.2 ASPH lens. The snapshots were stored for further measurements or for double check whenever needed.However, only prawns with complete rostrum have been manually straightened on millimeter paper to avoid the natural curvature of prawn body.…”

Section: Sampling and Biometric Analysesmentioning

confidence: 99%

“…Our team members are directly involved in both morphological and genetic description, and authentication of the Egyptian decapod species that exist within the Egyptian territories, being either autochthonous or allochthonous species (e.g. Abbas et al, 2016;Sharawy et al, 2017). Again, as M. latisulcatus is the most economically important species for the Egyptian capture fisheries in the Suez Gulf, it has been of a prime importance to develop both molecular and morphological tools for its exploration and authentication.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and biometric characterizations of the Western king prawn Melicertus latisulcatus (Kishinouye, 1896) in the Egyptian Red Sea.

2018

Egypt. J. of Aquatic Biolo. and Fish.

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“…(Rebello, Joseph, & Silvester, ) used biochemical methods (protein and enzyme profile) of P. semisulcatus to detect population structure. In other studies genetic diversity and genetic variation were determined in P. semisulcatus using mtDNA genes by the PCR‐RFLP method (Alam, De Croos, & Pálsson, ; Jahromi, Othman, & Rosazlina, ; Khamnamtong, Klinbunga, & Menasveta, ; Munasinghe & Senevirathna, ; Rezvani, Babaei, & Pourkazemi, ; Sharawy et al, , ). The limitation of mtDNA is maternally inherited and considered as a single locus.…”

Section: Introductionmentioning

confidence: 99%

Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers

Hassanien

Alrashada

2019

Aquac Res

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In this study, three molecular markers including random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) were used to evaluate genetic variation of green tiger prawn Penaeus semisulcatus collected from two geographically isolated environments; located in the Manifa, Arabian Gulf, Saudi Arabia and Ataka, Suez Gulf, Egypt. Genetic parameters included the percentage of polymorphism (P%), effective alleles (Ne), Nei genetic diversity (H) and Shannon index (I), which were calculated based on molecular data. All three marker systems distinguished genetic variation of P. semisulcatus in various levels. The highest polymorphism (91.30%) was obtained with SSR, followed by ISSR (82.26%) and RAPD markers (62.04%), respectively. Our results indicate that SSR appeared to be the best suited molecular assay for assessing the genetic variation between genotypes of P. semisulcatus. The present study indicated that Manifa and Ataka genotypes were closely related. Moreover, the analysis of variability could require more than one DNA‐based molecular marker techniques.

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Descriptive analysis, DNA barcoding and condition index of Penaeids (Crustacea: Decapoda) from the Egyptian Mediterranean coast

Cited by 12 publications

References 34 publications

Morphological and molecular clues for recording the first appearance of Artemia franciscana ( ) in Egypt

Morphological and molecular clues for recording the first appearance of Artemia franciscana ( ) in Egypt

Molecular and biometric characterizations of the Western king prawn Melicertus latisulcatus (Kishinouye, 1896) in the Egyptian Red Sea.

Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers

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