Desenvolvimento de meio de cultura semi-seletivo para detecção de Xanthomonas axonopodis pv. malvacearum em sementes de algodão (Gossypium hirsutum L.)

Abstract

Development of a semi-selective medium to detection Xanthomonas axonopodis pv. malvacerum from cotton seeds (Gossypium hirsutum L.) One of the main limiting factors of the cotton production is the occurrence of diseases. The bacteria are among the most important pathogens causing significant losses in the production. Cotton bacterial blight is caused by the bacterium Xanthomonas axonopodis pv. malvacearum (Xam). Is a serious disease that affects cotton and has worried world producers. The main source of inoculum for this bacterium is the infected seed. This work had as objective the development of a semi-selective medium to detect Xanthomonas axonopodis pv malvacearum in cotton seeds for routine tests in seed pathology laboratories. By fungitoxicity tests, basead on qualitative and quantitative antibiograms, it was idealized a semi-selective medium with the folloing composition: peptone (5.0 g/L); agar (15.0 g/L); meat extract (3.0 g); starch (10.0 g/L); violet crystal (150.0 µL violet crystal solution at 1%); water (1,000 mL); CaCl 2 (0.25 g); Tween 80 (10.0 mL/L). This medium has small effect on Xam and allows to isolate cotton seeds pathogens.

Ocorrência De Pseudomonas Cichorii Em Tomateiro No Estado De São Paulo

“…Também foi realizado o teste de imunofluorescência indireta, empregando-se a metodologia utilizada por DEZORDI (2006). Foram utilizados dois antissoros policlonais obtidos a partir do isolado tipo de P. cichorii IBSBF 1784 de chicória (título 1:16) e do isolado IBSBF 2309 obtido de plantas de tomateiro (título 1:16).…”