Desenvolvimento de membranas porosas à base de quitosana e laponita para tratamento de lesões de pele

Abstract

Skin lesions represent a great challenge for health professionals, since the most modern wound dressings available today are imported and expensive. Thus, there is a need to achieve more accessible resources for the population. The main challenge of skin regeneration research is the development of effective biomaterials that present biological activities as fungicide and bactericide, combining simple and low cost manufacturing technologies. In this context, this work presents the development of porous membrane based on chitosan/laponite nanocomposite for potential application in skin regeneration. Firstly, the commercial chitosan was purified and characterized in relation to the average degree of acetylation and the average viscosity molar mass. The freeze-drying process was used to produce porous membranes with different content of clay. The influence of laponite concentration on the membrane properties was also evaluated. X-Ray Diffraction (XRD) was used to determine the structure of the nanocomposite. Porous membranes were characterized by Scanning Electron Microscopy (SEM), thickness measurements, swelling capacity, thermogravimetric and mechanical analyses. The results revealed the increase in the porosity and in the thermal stability in addition to the decrease of the swelling capacity with the increase of clay content. In vitro tests were also carried out to evaluate the biocompatibility of the materials, such as: bioadhesion, antibacterial activity, viability and cell adhesion. Thereby, the results indicated that the porous membranes are biomaterials potentially useful as wound dressings.

Analysis of hydrophilicity degree in chitosan membranes produced from the incorporation of extract from the stalk of Anacardium microcarpum Ducke

“…The high values of the standard deviation are due to the method used in the manufacture of the membranes, which was solvent evaporation, already pointed out in the literature as the cause of this great dispersion (Sobral, 2000;Assis & Silva, 2003;Santos, 2018). The thickness obtained was satisfactory, since the dressings must have a thickness compatible with that of the skin, generally being smaller than the dermis (Gonzaga, 2018). The epidermis has a thickness that varies from 0.07 mm to 1.4 mm and the dermis, which varies from 1 to approximately 3 mm, depending on the part of the body (Dallan, 2005), and the membranes produced have thicknesses lower than the respective values, even lower than the thickness of the wound dressing marketed with collagen and alginate FIBRACOL*PLUS by Systagenix Wound Management Limited, which is 1 mm thick (Santos, 2018).…”

Study of Chytosan Membranes produced form the incorporation of extract of Anacardium Occidentale Linn

“…Assim como outros órgãos do corpo humano, a pele contém propriedades específicas. A espessura é uma dessas, ao ser diminuída por uma lesão, precisa ser reconstruída rapidamente, Segundo Gonzaga (2018), a literatura não especifica uma espessura padrão para as membranas, pois, esta propriedade baseia-se na área do corpo a ser tratada. Logo, a espessura deve ser mais fina que a derme humana, na qual o valor varia de 0,5 a 2,0 mm (500 a 2000µm), de acordo com o sexo e idade do indivíduo.…”