This study aimed to evaluate the attributes of natural fertility and intrinsic fragility of the soils of Ivinhema River Basin (BHI), to subsidize agricultural development projects and environmental control and monitoring bodies. For this purpose, 62 soil samples were collected, at a depth of 0 to 20 cm, in areas of native vegetation belonging to the state of Mato Grosso do Sul – Brazil. The characteristic climate of the study area is of the humid mesothermal subtype (Cfa) without drought. The predominant vegetation is of the Cerrado type and the geological substrate is predominantly composed of the Serra Geral and Caiuá Formation. The samples were air-dried and the physical attributes (sand, silt, and clay) and chemical variables (pH H2O, CaCl2, KCl, ∆pH, Al3+, m%, H + Al, Ca2+, Mg2+, K+, CEC, SB, V%, OC, P, Cu, Fe, Mn, and Zn) were determined according to methodologies in Embrapa's soil analysis methods manual. The data obtained were evaluated by descriptive statistics and principal component analysis. The results showed a great variety of soils inside the basin, whose natural fertility was high for the Leptsol, Regosol, and Nitisol soils, and low for the Argiluvic Plinthosol, Petroplinthic Plinthosol, and Planosol soils. The sandy soils showed lower levels of organic carbon and negative surface loads, which shows less capacity to retain cations essential to plant nutrition and less retention of environmental contaminants if any. Regarding the textural fragility of the soils of BHI, it was possible to verify the high sensitivity of the soil to erosion and susceptibility to the contamination of groundwater or infiltrated water. The data shown in research can fill the gaps of knowledge and/or for adapting conservation practices of agricultural soils that are more susceptible to erosive processes, as well as for environmental control and monitoring bodies and/or studies of area recovery and maintenance of soil fertility.