Desiccation‐time limits of photosynthetic recovery in <i>Equisetum hyemale</i> (Equisetaceae) spores

Lebkuecher, Jefferson G.

doi:10.2307/2445815

Cited by 29 publications

(20 citation statements)

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“…Desiccation-tolerant spores have been reported from pteridophytes (e.g. 33 fern species with nonchlorophyllous spores and five fern species with chlorophyllous spores; Pence 2000) and from the distantly related sphenophyte Equisetum hyemale L. (spore PDT approximately -525 MPa, Lebkuecher 1997). Desiccationtolerant spores are common in the mosses dispersed across oceans (Van Zanten 1978).…”

Section: Desiccation-tolerance In Other Photosynthetic Organismsmentioning

confidence: 99%

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

Gaff

Oliver²

2013

Functional Plant Biol.

190

176

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Abstract. In a minute proportion of angiosperm species, rehydrating foliage can revive from airdryness or even from equilibration with air of~0% RH. Such desiccation tolerance is known from vegetative cells of some species of algae and of major groups close to the evolutionary path of the angiosperms. It is also found in the reproductive structures of some algae, moss spores and probably the aerial spores of other terrestrial cryptogamic taxa. The occurrence of desiccation tolerance in the seed plants is overwhelmingly in the aerial reproductive structures; the pollen and seed embryos. Spatially and temporally, pollen and embryos are close ontogenetic derivatives of the angiosperm microspores and megaspores respectively. This suggests that the desiccation tolerance of pollen and embryos derives from the desiccation tolerance of the spores of antecedent taxa and that the basic pollen/embryo mechanism of desiccation tolerance has eventually become expressed also in the vegetative tissue of certain angiosperm species whose drought avoidance is inadequate in microhabitats that suffer extremely xeric episodes. The protective compounds and processes that contribute to desiccation tolerance in angiosperms are found in the modern groups related to the evolutionary path leading to the angiosperms and are also present in the algae and in the cyanobacteria. The mechanism of desiccation tolerance in the angiosperms thus appears to have its origins in algal ancestors and possibly in the endosymbiotic cyanobacteria-related progenitor of chloroplasts and the bacteria-related progenitor of mitochondria. The mechanism may involve the regulation and timing of the accumulation of protective compounds and of other contributing substances and processes.

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Section: Desiccation-tolerance In Other Photosynthetic Organismsmentioning

confidence: 99%

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

Gaff

Oliver²

2013

Functional Plant Biol.

190

176

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“…The onset for lipid crystallization is indicated by a horizontal dashed line. The shaded area is the temperature range where lipid melting transitions were detected to speculate that the inability to recover from losses of photosystem II (PSII) core function upon rehydration, as time in the dry state progressed in the green spores of E. hyemale (Lebkuecher 1997), could be related to an accumulation of oxidative damage in dry spores. Light is absorbed by the pigments of the photosynthetic apparatus of such spores when they are stored dry, and it is known that when water is absent, parts of PSII threaten to oxidise components of the photosynthetic apparatus, particularly when the antioxidant machinery is depleted (Kranner et al 2002(Kranner et al , 2008Illing et al 2005;Heber et al 2006;Farrant 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Light is absorbed by the pigments of the photosynthetic apparatus of such spores when they are stored dry, and it is known that when water is absent, parts of PSII threaten to oxidise components of the photosynthetic apparatus, particularly when the antioxidant machinery is depleted (Kranner et al 2002(Kranner et al , 2008Illing et al 2005;Heber et al 2006;Farrant 2007). An improved understanding of the ageing mechanisms of green spores during dry storage could therefore benefit from an investigation of the activity of specific (particularly enzymic) antioxidants in these spore in relation to the loss of function of the photosystems (Lebkuecher 1997;Kranner and Birtic 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The extremely short viability of the green spores of Equisetum hyemale has been attributed to the inability to recover core function of part of the photosynthetic apparatus upon rehydration after dry storage (Lebkuecher 1997). In other organisms tolerant to desiccation that retain chlorophyll in the dry state light is absorbed by the pigments of the photosynthetic apparatus, and it is known that when water is absent, free radicals are produced and threaten to oxidise components of the photosynthetic apparatus (Heber et al 2006;Kranner et al 2008).…”

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confidence: 99%

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Photo-oxidation modulates green fern spore longevity during dry storage

Ballesteros

Narayan

Varghese

et al. 2018

Plant Cell Tiss Organ Cult

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Desiccation tolerance and longevity of plant propagules in the dry state have significant implications for biotechnological applications. In this study fern spores were used as a unicellular model to characterize some of the mechanisms of ageing during dry storage of plant propagules (at relative humidity ca. 15%). More specifically, we compared the potential relationships among indicators of photo-oxidative stress and spore viability during dry storage between green (chlorophyllous) spores of Todea barbara and non-green spores of Christella dentata. Green spores stored under the light aged faster than those stored in the dark, and faster than light-and dark-stored non-green spores of C. dentata. This rapid ageing in light-stored green spores was associated with significantly lower antioxidant activity (relative to time zero and dark-stored spores) during storage, and a burst of hydrogen peroxide during the latter stages of storage, which was not a feature of dark-stored spores. We attribute these signs of enhanced oxidative-stress mediated ageing in light-stored spores to photo-oxidative processes, similar to those described in other homoiochlorophyllous organisms. Additionally, high antioxidant activity and low levels of reactive oxygen species in green spores compared with non-green spores suggests differing mechanisms of coping with life in the dry state.

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“…For comparison, our study revealed that the spore germination of O. regalis (99.9 %) was not affected even by desiccation to 4.6 % MC (eRH = 18.5 %). According to Lebkuecher (1997) the chlorophyllous spores of Equisetum hyemale can be desiccated almost completely following storage at 25°C and 2 % RH. Ninety percent of the spores survived that extreme, however, not longer than 1-weeklong, desiccation to 4 % relative water content.…”

Section: Discussion Desiccation Tolerance Of Green Sporesmentioning

confidence: 99%

The effect of moisture content and temperature on spore aging in Osmunda regalis

et al. 2015

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For spores, like seeds, the moisture level and storage temperature seem to be crucial factors responsible for their survival. However, in chlorophyllous spores in particular, the problem is not yet fully understood. The aim of this study was to investigate the effect of moisture content (MC; 6.6 and 4.6 %) and storage temperature (15 and -196°C) on spore germination and gametophyte development in Osmunda regalis. The MC (fresh weight basis) in freshly released spores was 7.5 %, and decreased to 6.6 and 4.6 % during 2 weeks of spore storage at 42 and 18 % relative humidity, respectively. Those spores germinated at 99.9 % within 28 h. The spores with 6.6 and 4.6 % MC were used for the storage at 15 and -196°C. The deterioration of spores, maintained with 6.6 % MC, proceeds within 1.5 years of storage at 15°C, starting with month 8. For the spores with 4.6 % MC serious disturbances in germination and capacity to gametophyte development were postponed for more than 6 months. The time required for spore germination increased with the age of the spores, ranging from 28 h in fresh spores to 10 days in spores stored for more than 11 months. Following 7-year-long cryostorage, spore viability remained at 99.9 %, the time taken for germination remained unaltered and gametophyte development was normal. The viability and vigor of spores were directly correlated with their age, the moisture content and storage temperature.Keywords Germination Á Liquid nitrogen Á Long-term cryostorage Á Spore equilibrium relative humidity

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Desiccation‐time limits of photosynthetic recovery in Equisetum hyemale (Equisetaceae) spores

Cited by 29 publications

References 50 publications

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

Photo-oxidation modulates green fern spore longevity during dry storage

The effect of moisture content and temperature on spore aging in Osmunda regalis

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