Design a highly specific sequence for electrochemical evaluation of meat adulteration in cooked sausages

Mansouri, Maryam; Khalilzadeh, Balal; Barzegari, Aboulfazl; Shoeibi, Shahram; Işildak, Selim; Bargahi, Nasrin; Omidi, Yadollah; Dastmalchi, Siavoush; Rashidi, Mohammad‐Reza

doi:10.1016/j.bios.2019.111916

Cited by 38 publications

(18 citation statements)

References 55 publications

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“…Compared with the LOD of multiplex PCR assays shown in Table 3 , the developed technique is qualified for discriminating meat source. Molecular authentication or molecular traceability of meat species, which is based on the developed multiplex PCR amplification of genomic DNA, has provided an accurate analysis of meat ingredients ( 22 , 23 ). In this regard, the proposed PCR method targeting mtDNA to authenticate twelve animal species in food products would be especially useful.…”

Section: Discussionmentioning

confidence: 99%

Molecular Authentication of Twelve Meat Species Through a Promising Two-Tube Hexaplex Polymerase Chain Reaction Technique

et al. 2022

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Frequent meat frauds have aroused significant social attention. The aim of this study is to construct a two-tube hexaplex polymerase chain reaction (PCR) method offering accurate molecular authentication of twelve meat species in actual adulteration event. Deoxyribonucleic acid (DNA) sequencing demonstrates that designed primers can specifically amplify target species from genomic DNA mixture of six species in each tube reaction, which showed 100% accuracy of horse (148 bp), pigeon (218 bp), camel (283 bp), rabbit (370 bp), ostrich (536 bp), and beef (610 bp) as well as turkey (124 bp), dog (149 bp), chicken (196 bp), duck (277 bp), cat (380 bp), and goose (468 bp). A species-specific primer pair produced the target band in the presence of target genomic DNA but not non-target species. Through multiplex PCR assays with serial concentration of the DNA mixture of six species in each PCR reaction, the detection limit (LOD) of the two-tube hexaplex PCR assay reached up to 0.05–0.1 ng. Using genomic DNA isolated from both boiled and microwave-cooked meat as templates, PCR amplification generated expected PCR products. These findings demonstrate that the proposed method is specific, sensitive and reproducible, and is adequate for food inspection. Most importantly, this method was successfully applied to detect meat frauds in commercial meat products. Therefore, this method is of great importance with a good application foreground.

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Section: Discussionmentioning

confidence: 99%

Molecular Authentication of Twelve Meat Species Through a Promising Two-Tube Hexaplex Polymerase Chain Reaction Technique

et al. 2022

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“…The techniques have been continuously evolved for identifying the origin of meat species in the recent years (2,(12)(13)(14)(15). DNA molecules present in every cell and possess high stability, and therefore, DNA-based techniques combined with polymerase chain reaction (PCR) provide more reliable methods in discriminating meat species (13,16,17).…”

Section: Introductionmentioning

confidence: 99%

A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials

Zhou

Zhong²,

Zhou³

et al. 2022

Front. Nutr.

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Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for the identification of meat products in food industries. However, relatively less is known about a multiplex PCR method authenticating seven animal species simultaneously in one reaction due to technological challenge. Through screening new species-specific primers and optimizing PCR system, a heptaplex PCR method was established, which could simultaneously detect seven meat ingredients of camel (128 bp), pigeon (157 bp), chicken (220 bp), duck (272 bp), horse (314 bp), beef (434 bp), and pork (502 bp) in a single-tube reaction. DNA sequencing solidly validated that each set of primers specifically amplified target species from total DNA mixtures of seven meat species. The developed multiplex assay was stable and sensitive enough to detect 0.01–0.025 ng DNA from various meat treatments including raw, boiled, and autoclaved meat samples or target meat content of 0.1% total meat weight, suggesting the suitability of the heptaplex PCR technique for tracing target meats with high accuracy and precision. Most importantly, a market survey validated the availability of this multiplex PCR technique in real-world meat products with a good application foreground.

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“…Meat authentication is an important concern to protect consumers from illegal and unwanted ingredients [ 1 , 2 , 3 , 4 ]. However, meat adulteration such as unlisted, mislabeled or fraudulent ingredients has frequently been reported around the world and has become a severe global issue [ 4 , 5 ]. Although some laws have been enacted for ensuring the quality and safety of meat products, adulteration is still widespread due to the purpose of economic pursuit [ 1 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the techniques for authenticating meat species have been continuously evolving. Many analytical methods of biological, immunological, physical, chemical, anatomical and histological analyses have been developed [ 2 , 3 , 4 , 7 , 12 ]. Among them, protein-based methods are widely used to identify meat species in composite mixtures [ 5 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Proteins are likely to be degraded, denatured or damaged in processed meat products, limiting the accuracy of the identification of meat species in thermally treated foods [ 13 , 14 ]. In comparison, DNA-based analytical methods coupled with polymerase chain reaction (PCR) present a reliable alternative to protein-based methods in the discrimination and mislabeling detection of meat species, as DNA molecules possess high stability and are present in every type of cell [ 4 , 5 ]. Both multiplex and real-time PCR techniques are highly specific and efficient in the identification of meat adulteration [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species

Cai

Zhou

Liu

et al. 2021

Foods

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Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp) and duck (718 bp) in one reaction. The proposed method has been validated to be specific, sensitive, robust and inexpensive. Taken together, the developed septuple PCR assay is reliable and efficient, not only to authenticate animal species in commercial meat products, but also easily feasible in a general laboratory without special infrastructures.

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Design a highly specific sequence for electrochemical evaluation of meat adulteration in cooked sausages

Cited by 38 publications

References 55 publications

Molecular Authentication of Twelve Meat Species Through a Promising Two-Tube Hexaplex Polymerase Chain Reaction Technique

Molecular Authentication of Twelve Meat Species Through a Promising Two-Tube Hexaplex Polymerase Chain Reaction Technique

A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials

A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species

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