Design and applications of coupled SPE‐CE

Tempels, F. W. Alexander; Underberg, W.J.M.; Somsen, Govert W.; Jong, Gerhardus J. de

doi:10.1002/elps.200700149

Cited by 51 publications

(58 citation statements)

References 176 publications

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“…Additionally, as the SPE column is physically separated from the CE capillary in the on-line system, this allows for independent operation and optimization of the SPE and the CE. [2][3][4][5][6] On-line coupling of SPE with CE requires a specially designed interface. In addition to implementation of electric connection for CE, the interface should have the ability to exactly transfer nanoliters of fractions from SPE onto CE with minimized degradation of the efficiencies of SPE and CE.…”

Section: Introductionmentioning

confidence: 99%

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

Weng

et al. 2014

ANAL. SCI.

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A miniaturized transverse flow gating interface (μ-TFGI) is presented for the on-line coupling of solid-phase extraction with capillary electrophoresis (SPE-CE). The fabrication and operation procedures of the μ-TFGI are described in detail. After the operation conditions were investigated and selected, the μ-TFGI was evaluated on an SPE-CE-UV setup using clenbuterol (CLB) as the test analyte. The preconcentration factors obtained with the SPE-CE-UV system and the SPE-UV part were 600 and 620, respectively. The plate numbers obtained within 3 min were 100000 and 80000 for automatic injection via the μ-TFGI and manual direct injection, respectively. The precisions were 2.4 -6.8% (RSD, %, n = 9) and 3.1% (RSD, %, n = 27) for the recovery (94.3 -101.9%) and migration time of CLB spiked in urine samples, respectively. These results demonstrate that the μ-TFGI has the ability to exactly and reproducibly transfer nanoliters of fractions from SPE onto CE with no degradation of the efficiencies of SPE and CE.

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Section: Introductionmentioning

confidence: 99%

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

Weng

et al. 2014

ANAL. SCI.

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“…To overcome this limitation, improvements in UV detection were proposed using longer optical path lengths with a Z-/ U-shaped or a bubble detection cell. More sensitive detection methods, such as laser-induced fluorescence or mass spectrometry are also frequently used [2,3]. Alternative strategies consist in preconcentrating the analyte before CE separation, either by electrophoretic stacking or by solid-phase extraction (SPE) [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…More sensitive detection methods, such as laser-induced fluorescence or mass spectrometry are also frequently used [2,3]. Alternative strategies consist in preconcentrating the analyte before CE separation, either by electrophoretic stacking or by solid-phase extraction (SPE) [3][4][5]. In stacking methods, the volume of the sample must be lower than the total capillary volume [6].…”

Section: Introductionmentioning

confidence: 99%

Bubble cell for magnetic bead trapping in capillary electrophoresis

Gassner

Proczek

2011

Anal Bioanal Chem

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A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments.

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“…Although attomole limits of detection has been reported in CE-ESI-MS applications [3], the concentration detection limit of CE-ESI-MS is still poor because the volume that can be injected to CE is very limited [4]. To improve the concentration detection limit, various preconcentration approaches have been proposed [5][6][7][8][9][10][11][12][13]. In electrophoresis-based methods [5][6][7], which utilize a stacking technique, preconcentration occurs in the separation capillary.…”

Section: Introductionmentioning

confidence: 99%

The development of a two‐leveled two cross interface for on‐line coupling solid‐phase extraction and capillary electrophoresis‐mass spectrometry

Lee

Her

2009

Electrophoresis

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A two-leveled, two cross PDMS-based interface for on-line coupling of SPE with CE-MS was proposed. In this interface, the SPE column and the CE separation column were positioned orthogonally and two crosses were fabricated on the interface. With the two cross design, the operation of SPE could be performed independently without unexpected flow through leakage into the separation column. The performance of the interface was optimized using a peptide mixture. The position of the SPE column related to the CE separation channel was found to be critical to the performance of the system. Under the optimal position, the separation efficiency was similar to a CE-MS experiment without SPE. The peptide signals were enhanced 50- to 100-fold and the repeatability was within 4% RSD for migration time and 10% RSD for peak area. A tryptic digest of cytochrome c was used to demonstrate the feasibility of the interface in protein identification at a level of 1 ng/microL.

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Design and applications of coupled SPE‐CE

Cited by 51 publications

References 176 publications

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

Bubble cell for magnetic bead trapping in capillary electrophoresis

The development of a two‐leveled two cross interface for on‐line coupling solid‐phase extraction and capillary electrophoresis‐mass spectrometry

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