Design and applications of protein delivery systems in nanomedicine and tissue engineering

Bizeau, Joëlle; Mertz, Damien

doi:10.1016/j.cis.2020.102334

Cited by 31 publications

(21 citation statements)

References 229 publications

(494 reference statements)

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“…Bioactive substances (BAS) treatment using small molecule drugs, proteins, RNA, cells, etc, was developed and proved to be an efficient theraputic solution for saving their lives ( Farokhzad and Langer, 2009 ; Kamaly et al, 2016 ). Neverthless, the therapeutic effect is lmitted owning to the inherent characteristics of BAS, such as low bioavailability, cytotoxicity, poor physico-chemical stability and so on ( Jain et al, 2013 ; Riley and Vermerris, 2017 ; Bizeau and Mertz, 2020 ; Nkanga et al, 2020 ). More importantly, the traditional BAS delivery systems also have some limitations such as over-dose and/or uncontrollable release of BAS, first-pass effect, etc.…”

Section: Introductionmentioning

confidence: 99%

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

Zhang

Liu

Hao

et al. 2022

Front. Bioeng. Biotechnol.

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Bioactive substances (BAS), such as small molecule drugs, proteins, RNA, cells, etc., play a vital role in many therapeutic applications, especially in tissue repair and regeneration. However, the therapeutic effect is still a challenge due to the uncontrollable release and instable physico-chemical properties of bioactive components. To address this, many biodegradable carrier systems of micro-nano structures have been rapidly developed based on different biocompatible polymers including polyhydroxyalkanoates (PHA), the microbial synthesized polyesters, to provide load protection and controlled-release of BAS. We herein highlight the developments of PHA-based carrier systems in recent therapeutic studies, and give an overview of its prospective applications in various disease treatments. Specifically, the biosynthesis and material properties of diverse PHA polymers, designs and fabrication of micro- and nano-structure PHA particles, as well as therapeutic studies based on PHA particles, are summarized to give a comprehensive landscape of PHA-based BAS carriers and applications thereof. Moreover, recent efforts focusing on novel-type BAS nano-carriers, the functionalized self-assembled PHA granules in vivo, was discussed in this review, proposing the underlying innovations of designs and fabrications of PHA-based BAS carriers powered by synthetic biology. This review outlines a promising and applicable BAS carrier platform of novelty based on PHA particles for different medical uses.

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Section: Introductionmentioning

confidence: 99%

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

Zhang

Liu

Hao

et al. 2022

Front. Bioeng. Biotechnol.

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“…1,2 Also, to facilitate the functional accuracy of the regenerated tissues, there is an increasing awareness of the need to delivery these protein-based message molecules, including large families of growth factors and cytokines, to the target cell population that mimic their endogenous production profiles. 3,4 However, once administered, the circulatory half-lives of these protein drugs or cytokines are short compared to the time required for therapeutic efficacy or cell response. Furthermore, proteins are fragile conformational macromolecular structures, susceptible to unfolding and to enzymatic degradation.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the idea of protein delivery system to protect, carry and release labile therapeutic proteins or growth factors has been extensively adopted. 3,5 These protein controlled release systems can be formulated in a wide range of dimensions, such as nano/micro particles, fibers, films, patches and macroporous scaffolds. The protein release mechanism from drug delivery systems includes natural diffusion, hydrolytic erosion or local biochemical stimuli (pH, redox, enzymes).…”

Section: Introductionmentioning

confidence: 99%

“…To date, these delivery systems have shown great potential for cancer treatment, diabetes treatment, gene delivery, tissue regeneration and many other applications. 3 The advent of 3 D printing technology in the pharmaceutical industry made it possible to design and manufacture novel complex dosage form with tailoring dose size and drug release profile adapted to patients' specific needs. Furthermore, customizing polypills using 3 D printing technology, where multiple drugs, having different release characteristics, can formed into a single tablet for oral administration, such tablets possessing complex shapes and geometries cannot be manufactured by the conventional powder compaction technique.…”

Section: Introductionmentioning

confidence: 99%

“…To date, these delivery systems have shown great potential for cancer treatment, diabetes treatment, gene delivery, tissue regeneration and many other applications. 3…”

Section: Introductionmentioning

confidence: 99%

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3D-printed construct from hybrid suspension as spatially and temporally controlled protein delivery system

et al. 2021

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Protein delivery systems have been extensively applied in controlled releasing of protein or polypeptides for therapeutic treatment or tissue regeneration. While 3 D printing technology shows great promise in novel dosage form with tailoring dose size and drug release profile, 3 D printable protein delivery system has to face many difficult challenges. In this study, we developed a hybrid suspension combining Eudragit polyacrylate colloid as matrix material and Pluronic polyether hydrogel as diffusion channel for protein release. This hybrid suspension can be 3 D-printed into construct with complex shape and inner structures thanks to its pseudoplastic and thixotropic rheological properties. The protein can be incorporated in hybrid suspension either in its original or nanoparticle capsulated form. The experiment shows that the protein release from construct is a function of drying time, molecular weight (MW) of chitosan, as well as their own structural/diffusional properties. Also, the theoretical derivation suggests polyacrylate matrix tortuosity, chitosan erosion rate as well as hydrogel diffusion coefficient all contributed to the extended duration of release profile. In addition, cytotoxicity test through cell culture confirmed that the construct fabricated from hybrid suspension exhibit relative good bio-compatibility. Finally, heterogeneous constructs with zoned design were fabricated as protein delivery system, which demonstrated the capability of hybrid suspension technique for spatial and temporal release of macromolecular drugs to realize pharmaceutical effectiveness or guild cell organization.

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Nanomaterial Strategies for Delivery of Therapeutic Cargoes

Ledesma

Ozcan

Sun

et al. 2021

Adv Funct Materials

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Over the last decade, much progress has been made in developing nanoparticle-mediated delivery systems to overcome the limitations of existing in vivo delivery technologies. However, the balance between efficacy and safety continues to limit the clinical translation of nanoscale delivery systems. Furthermore, optimizing delivery efficiency requires tuning nanoparticle type and attachment chemistry, both of which are dependent on the cargo being delivered. While the delivery of protein therapeutics is of particular interest, the complexity inherent to protein cargo introduces additional challenges for cargo loading and stabilization. Advances needed for efficient and safe in vivo nanoparticle delivery systems should prioritize design strategies that co-optimize safety, biodegradability, and covalent functionalization. In this review, the most commonly used non-viral nanoparticles are outlined for delivery of small molecule drug, nucleic acid, and protein therapeutic cargoes and potential strategies are discussed for rationally designing nanoparticle-mediated delivery systems.

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Design and applications of protein delivery systems in nanomedicine and tissue engineering

Cited by 31 publications

References 229 publications

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

3D-printed construct from hybrid suspension as spatially and temporally controlled protein delivery system

Nanomaterial Strategies for Delivery of Therapeutic Cargoes

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