Design and Cellular Fate of Bioinspired Au–Ag Nanoshells@Hybrid Silica Nanoparticles

Abstract

Silica-coated gold-silver alloy nanoshells were obtained via a bioinspired approach using gelatin and poly-l-lysine (PLL) as biotemplates for the interfacial condensation of sodium silicate solutions. X-ray photoelectron spectroscopy was used as an efficient tool for the in-depth and complete characterization of the chemical features of nanoparticles during the whole synthetic process. Cytotoxicity assays using HaCaT cells evidenced the detrimental effect of the gelatin nanocoating and significant induction of late apoptosis after silicification. In contrast, PLL-modified nanoparticles had less biological impact that was further improved by the silica layer, and uptake rates of up to 50% of those of the initial particles could be achieved. These results are discussed considering the effect of nanosurface confinement of the biopolymers on their chemical and biological reactivity.

Doxorubicin-Conjugated Bimetallic Silver–Gadolinium Nanoalloy for Multimodal MRI-CT-Optical Imaging and pH-Responsive Drug Release

“…In recent years, bimetallic nanoparticles (BNPs) elucidate special attention because of their unique properties in comparison to respective monometallic systems. − Silver–gold BNPs are exclusively reported for specific detection and therapy of cancer. , Successful synthesis of europium and gold (Eu–Au) BNPs through bioreduction using plant extract has been reported . Au–Fe nanoalloy has been reported as an excellent candidate for multimodal MRI-CT-SERS imaging. , Because of unique luminescence and magnetic properties, much interest has been devoted on bimetallic lanthanide bioprobes for applications in cellular imaging, cancerous cell detection and DNA tagging. − A recent report emphasize the synthesis of Ag–Dy BNPs as a theranostic device for MRI-CT-NIR multimodal imaging and drug delivery to cancer cells …”

A Novel Strategy for the Detection and Quantification of Nanoplastics by Single Particle Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

“…Type A gelatin is a protein composed of a set of amino acids, containing NH 2 and COOH groups. Due to the protonation state of acid (carboxylates) and basic (amines) functions, gelatin provides a net global positive charge (isoelectric point  8) at the working pH (6-8) 39 . Therefore, the labelling is achieved through electrostatic interactions between the global negative charge bearing by the deprotonated carboxylate groups from the surface of nanoplastics, and the positive charge of gelatin-coated gold nanoparticles at neutral pH.…”

MnO2-gated Nanoplatforms with Targeted Controlled Drug Release and Contrast-Enhanced MRI Properties: from 2D Cell Culture to 3D Biomimetic Hydrogels

“…At the Mn 2p level (Figure 3b), the two peaks at 642 eV and 653.8 eV unambiguously sign for the presence of MnO 2 , as also confirmed by the peak at 530.1 eV on the O 1s spectrum [47] (Figure 3d). The peaks at 103.2 eV on the Si 2p spectrum (Figure 3c) and 532.4 eV on the O 1s spectrum are characteristic of hydrated silica [48]. Deconvoluted spectra at the C 1s and N 1s levels evidenced the presence of C-C, C-O, C=O, C-N and N-C=O groups ( Figure S4).…”