Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription–Translation System
Angelica Rose Galvan,
Christopher M. Green,
Shelby L. Hooe
et al.
Abstract:DNA nanotechnology has made initial progress toward developing gene-encoded DNA origami nanoparticles (NPs) that display potential utility for future gene therapy applications. However, due to the challenges involved with gene delivery into cells including transport through the membrane, intracellular targeting, and inherent expression of nucleases along with interference from other active proteins, it can be difficult to more directly study the effect of DNA NP design on subsequent gene expression. In this wo… Show more
Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures for optimal protein expression.
Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures for optimal protein expression.
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