Design and characterization of an in vivo injectable hydrogel with effervescently generated porosity for regenerative medicine applications

Griveau, Louise; Lafont, Marianne; Goff, Héloïse Le; Drouglazet, Clémence; Robbiani, Baptiste; Berthier, Aurore; Sigaudo‐Roussel, Dominique; Latif, Najma; Visage, Catherine Le; Gache, Vincent; Debret, Romain; Weiss, Pierre; Sohier, Jérôme

doi:10.1016/j.actbio.2021.11.036

Cited by 39 publications

(24 citation statements)

References 69 publications

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“…In situ gelation can realize precise conformation to irregular cavities, and result in excellent integration and retention rates in the injection sites [ 102 , 103 ]. For example, entrapping effervescently generated CO 2 bubbles can help to form highly interconnected porous networks in injectable hydrogels in vivo, which is conducive to cellular attachment, infiltration, proliferation, and ECM deposition [ 104 ]. Polymers and exosomes are incorporated before the addition of crosslinkers for gelation.…”

Section: Exosome-loading Strategiesmentioning

confidence: 99%

Hydrogels for Exosome Delivery in Biomedical Applications

Xie

Guan

Guo

et al. 2022

Gels

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Hydrogels, which are hydrophilic polymer networks, have attracted great attention, and significant advances in their biological and biomedical applications, such as for drug delivery, tissue engineering, and models for medical studies, have been made. Due to their similarity in physiological structure, hydrogels are highly compatible with extracellular matrices and biological tissues and can be used as both carriers and matrices to encapsulate cellular secretions. As small extracellular vesicles secreted by nearly all mammalian cells to mediate cell–cell interactions, exosomes play very important roles in therapeutic approaches and disease diagnosis. To maintain their biological activity and achieve controlled release, a strategy that embeds exosomes in hydrogels as a composite system has been focused on in recent studies. Therefore, this review aims to provide a thorough overview of the use of composite hydrogels for embedding exosomes in medical applications, including the resources for making hydrogels and the properties of hydrogels, and strategies for their combination with exosomes.

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Section: Exosome-loading Strategiesmentioning

confidence: 99%

Hydrogels for Exosome Delivery in Biomedical Applications

Xie

Guan

Guo

et al. 2022

Gels

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“…Such a structure on the one hand gives COS/SA/nM 2+ PDF a higher specific surface area and water content. On the other hand, as a wound dressing it is conducive to absorbing wound exudate, transporting nutrients and excreting metabolic waste, as well as providing a certain three-dimensional space for cell adhesion and proliferation [18].…”

Section: Preparation Mechanism Of Cos/sa/nm 2+ Pdfmentioning

confidence: 99%

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

et al. 2022

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The development of an efficient and convenient material to improve skin tissue regeneration is a major challenge in healthcare. Inspired by the theory of moist wound healing, portable chitooligosaccharide (COS)/sodium alginate (SA) dual-net gel films containing multiple metal ions were prepared by a casting and in -situ spray method, which can be used to significantly promote wound healing without the use of therapeutic drugs. A variety of divalent cations was introduced in this experiment to improve the advantages of each metal ion by forming metal ion chelates with COS. Moreover, the physicochemical properties and antioxidant properties of nIon2+-COS/SA gel films were systematically characterized and evaluated by in vitro experiments. The gel films showed good antibacterial activity against Gram-negative and Gram-positive bacteria. In addition, the gel films showed good cytocompatibility in cellular experiments, and the gel films with Zn2+ and Sr2+ addition significantly accelerated wound healing in whole skin defect model experiments. Therefore, this nIon2+-COS/SA gel film is an ideal candidate material for wound dressing.

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“…Hydrogels are three-dimensional hydrophilic polymer networks that are able to absorb and retain a large amount of water without dissolving or losing their characteristic resistance properties [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. Since Wichterle and Lim provided this definition and proved their use in ophthalmology [ 8 ], their application has expanded to cover a wide range of fields such as agriculture [ 9 , 10 ], sensors [ 11 , 12 ], and water-treatment solutions [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes

et al. 2022

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Every year millions of people worldwide undergo surgical interventions, with the occurrence of mild or severe post-treatment consequences meaning that rehabilitation plays a key role in modern medicine. Considering the cases of burns and plastic surgery, the pressing need for new materials that can be used for wound patches or body fillers and are able to sustain tissue regeneration and promote cell adhesion and proliferation is clear. The challenges facing next-generation implant materials also include the need for improved structural properties for cellular organization and morphogenic guidance together with optimal mechanical, rheological, and topographical behavior. Herein, we propose for the first time a sodium alginate hydrogel obtained by a thiol–yne reaction, easily synthesized using carbodiimide chemistry in a two-step reaction. The hydrogels were formed in all cases within a few minutes of light irradiation, showing good self-standing properties under solicitation. The mechanical, rheological, topographical, and swelling properties of the gels were also tested and reported. Lastly, no cytotoxicity was detected among the hydrogels. Soluble extracts in culture media allowed cell proliferation, and no differences between samples were detected in terms of metabolic activity and DNA content. These results suggest the potential use of these cytocompatible hydrogels in tissue engineering and regenerative medicine.

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Design and characterization of an in vivo injectable hydrogel with effervescently generated porosity for regenerative medicine applications

Cited by 39 publications

References 69 publications

Hydrogels for Exosome Delivery in Biomedical Applications

Hydrogels for Exosome Delivery in Biomedical Applications

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

Photocurable Thiol–yne Alginate Hydrogels for Regenerative Medicine Purposes

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