Design and characterization of crotamine-functionalized gold nanoparticles

Karpel, Richard L.; Liberato, Michelle S.; Campeiro, Joana D’Arc; Bergeon, Lorna; Szychowski, Brian; Butler, Andrew; Marino, Giovanni; Cusic, Joelle F.; Oliveira, Lilian Caroline Gonçalves de; Oliveira, Eduardo B.; Farias, Marcelo Alexandre de; Portugal, Rodrigo Villares; Alves, Wendel A.; Daniel, Marie‐Christine; Hayashi, Mirian A. F.

doi:10.1016/j.colsurfb.2017.12.013

Cited by 16 publications

(8 citation statements)

References 31 publications

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“…Similarly, TEM was used for the characterization of the morphology and size of liposomes conjugated to CPP Pep-1, but internalization studies were undertaken by fluorescence (Jiao et al ., 2017). On the other part, crotamine conjugated gold nanoparticles additionally decorated with polyethylene glycol were internalized in the HeLa cells which was followed by TEM (Karpel et al ., 2018). A variant of TEM, cryo-TEM was used to follow penetratin conjugated liposomes crossing the blood–brain barrier, accumulated in vivo in the rat brain (Tremmel et al ., 2016).…”

Section: Biophysical Methods and Cppsmentioning

confidence: 99%

Studies of cell-penetrating peptides by biophysical methods

Zorko

Langel

2022

Quart. Rev. Biophys.

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Biophysical studies have a very high impact on the understanding of internalization, molecular mechanisms, interactions, and localization of CPPs and CPP/cargo conjugates in live cells or in vivo. Biophysical studies are often first carried out in test-tube set-ups or in vitro, leading to the complicated in vivo systems. This review describes recent studies of CPP internalization, mechanisms, and localization. The multiple methods in these studies reveal different novel and important aspects and define the rules for CPP mechanisms, hopefully leading to their improved applicability to novel and safe therapies.

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Section: Biophysical Methods and Cppsmentioning

confidence: 99%

Studies of cell-penetrating peptides by biophysical methods

Zorko

Langel

2022

Quart. Rev. Biophys.

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“…Samples analyzed by cryogenic transmission electron microscopy (cryo‐TEM) were prepared in a controlled environment vitrification system (Vitrobot Mark IV, Thermo Fischer Scientific) as discussed elsewhere . The analyses were carried out in low‐dose condition with a defocus range of −1 to −3 μm using a JEOL JEM‐1400 Plus (JEOL, Japan) electron microscope operating at 120 kV with a lanthanum hexaboride (LaB 6 ) filament.…”

Section: Methodsmentioning

confidence: 99%

Amphiphilic polylactide‐poly(ethylene oxide)‐poly(propylene oxide) block copolymers: Self‐assembly behavior and cell affinity

Loiola

Farias

Portugal

et al. 2018

J. Polym. Sci. Part A: Polym. Chem.

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Polylactide (PLA) is a biodegradable polyester recognized for its potential use as a biomedical material. Poly(ethylene oxide) (PEO) and copolymers based on PEO and poly(propylene oxide) (PPO) are biocompatible polyethers widely applied in the biomedical field, particularly as macromolecular nonionic surfactants. In this work, PLA blocks were attached to the PEO and to the PEO and PPO‐based triblock copolymer PEO–PPO–PEO, through ring‐opening polymerization of racemic lactide (rac‐LA) to obtain the amphiphilic triblock PLA–PEO–PLA and pentablock PLA–PEO–PPO–PEO–PLA copolymers containing hydrophilic/hydrophobic blocks with variable block mass ratios. The copolymers were evaluated for chemical composition, molar mass, and thermal properties, and they were used to prepare self‐assemble aggregates in water from tetrahydrofuran polymer solutions. The combination of scattering light experiments and microscopy techniques revealed the spherical morphology of the aggregates with diameters around 180–200 nm, which comprises a hydrophobic PLA core and a hydrophilic polyether shell. The aggregates are nontoxic to human cervical cancer cell line — HeLa cells, as determined by MTS assay, and the aggregates are potential candidates to be applied in the encapsulation of hydrophobic compounds. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018, 56, 2203–2213

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“…This toxin is specifically uptaken by actively proliferating cells, being able to permeate several lineages in vitro (Nascimento et al, 2007). Additionally, several molecules based on crotamine structure, including fluorescent derivatives (Tansi et al, 2019) and functionalized with gold nanoparticles (Karpel et al, 2018), for instance, are being developed. Crotamine and its analogues have been tested in healthy and tumorous cell lines, and the results indicate they can be used as selective delivery tools of anticancer molecules (Mambelli-Lisboa et al, 2018).…”

Section: Snakesmentioning

confidence: 99%