Design and characterization of microspheres for a 3D mesenchymal stem cell culture

Lastra, María Laura; Ribelles, J.L. Gómez; Cortizo, Ana Marı́a

doi:10.1016/j.colsurfb.2020.111322

Cited by 18 publications

(12 citation statements)

References 30 publications

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“…RAW 264.7 is a cell line derived from Mus musculus (mouse) macrophages that expresses different inflammatory markers (NO production, expression of NO synthases and Interleukins) when it is exposed to toxic and/or immunogenic substances. Thus, these cells are an excellent in vitro model to determine whether a new biomaterial is potentially inflammatory [37,38]. Wang et al have developed an interesting series of thermogels which have the ability to induce a controllable inflammatory capacity once implanted subcutaneously [39].…”

Section: Discussionmentioning

confidence: 99%

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

Torres

Oberti

Fernández

2021

Journal of Biomaterials Science, Polymer Edition

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Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.

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Section: Discussionmentioning

confidence: 99%

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

Torres

Oberti

Fernández

2021

Journal of Biomaterials Science, Polymer Edition

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“…In addition, 3D spheroid culture has great advantages in bone tissue regeneration. However, the strategies most common that have been developed in bone application is in combination with the use of organic and inorganic materials in order to optimize the functionality of 3D spheroid cultures: (i) the use of fumarate-vinyl acetate copolymer and chitosan microgels has been evaluated and they create a 3D environment that enables the spheroid to have an extracellular matrix, which allows it to reorganize and acquire mobility (36); and (ii) gelatin methacrylate (GelMA)/ Na2CO3-based microspheres have been developed to allow the formation of CaCo3 crystals, which increase cell adhesion as well as the expression of ALP, suggesting that this model can be applied in the engineering of tissues focused on the regeneration of bone tissue using 3D cultures (37).…”

Section: Discussionmentioning

confidence: 99%

3D Spheroid Cell Cultures and Their Role in Bone Regeneration: a Systematic Review

Cuevas-González

Suaste-Olmos

Cuevas-González

et al. 2021

Odovtos - Int J Dent Sc

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Recently, the 3D spheroid cell culture application has been extensively used in the treatment of bone defects. A wide variety of methodologies have been used, which has made the comparison of results complex. Therefore, this systematic review has two aims: (i) to perform an analysis focused on the role of 3D spheroid cell culture in bone regeneration strategies; and (ii) address the main challenges in clinical application. A search of the following keywords "3D cell culture", "spheroid", and "bone regeneration" was carried out in the PubMed, Scopus, and ScienceDirect databases and limited to the years 2010-2020. Studies were included if their primary objective was the behavior of cell aggregates to formed spheroids structures by different 3D cell culture techniques focused on the regeneration of bone tissue. To address the risk of bias for in vitro studies, the United States national toxicology program tool was applied, and descriptive statistics of the data were performed, with the SPSS V.22 program. A total of 16 studies were included, which met the established criteria corresponding to in vitro and in vitro/in vivo studies; most of these studies used stem cells for the 3D cell spheroids. The most often methods used for the 3D formation were low adherence surface and rotational methods, moreover, mesenchymal stem cells were the cell line most frequently used because of their regenerative potential in the field of bone tissue engineering. Although the advances in research on the potential use of 3D spheroids in bone regeneration have made great strides, the constant innovation in cell spheroid formation methodologies means that clinical application remains in the future as strategy for 3D tissue bioprinting.

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“…However, its LCST value is lower than body temperature which ranges from ∼30°C to 32°C, irrespective of polymer concentration. To raise the LCST of the polymer, frequently hydrophilic polymers are incorporated in the copolymer or increase the proportion of hydrophilic to hydrophobic components in the copolymer and have been shown to elevate the LCST of PNIPAm (Jain et al, 2015; Koc & Alveroglu, 2016; Osváth & Iván, 2017). Therefore, in this study, NIPAm was copolymerized with hydrophilic AAc or AAm at different ratios to prepare a thermo‐responsive PNIPAm‐ co ‐AAc with optimized LCST value near to body temperature.…”

Section: Resultsmentioning

confidence: 99%

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

Darge

Chuang

Lai

et al. 2021

Biotech & Bioengineering

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Enzymatic detachment of cells might damage important features and functions of cells and could affect subsequent cell‐based applications. Therefore, nonenzymatic cell detachment using thermosensitive polymer matrix is necessary for maintaining cell quality after harvesting. In this study, we prepared thermosensitive PNIPAm‐co‐AAc‐b‐PS and PNIPAm‐co‐AAm‐b‐PS copolymers and low critical solution temperature (LCST) was tuned near to body temperature. Then, spin coated polymer films were prepared for cell adhesion and thermal‐induced cell detachment. The alpha‐step analysis and scanning electron microscope image of the films suggested that the thickness of the films depends on the molecular weight and concentration which ranged from 206 to 1330 nm for PNIPAm‐co‐AAc‐b‐PS and 97.5–497 nm for PNIPAm‐co‐AAm‐b‐PS. The contact angles of the films verified that the polymer surface was moderately hydrophilic at 37°C. Importantly, RAW264.7 cells were convincingly proliferated on the films to a confluent of >80% within 48 h and abled to detach by reducing the temperature. However, relatively more cells were grown on PNIPAm‐co‐AAm‐b‐PS (5%w/v) films and thermal‐induced cell detachment was more abundant in this formulation. As a result, PNIPAm‐co‐AAm‐b‐PS (5%w/v) was further used to coat commercial cytodex 3 microcarriers for 3D cell culturing and interestingly enhanced cell detachment with preserved potential of recovery was observed at a temperature of below LCST. Thus, surface modification of microcarriers with thermosensitive PNIPAm‐co‐AAm‐b‐PS could be vital strategy for nonenzymatic cell detachment and to achieve adequate number of cells with maximum cell viability and functionality.

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Design and characterization of microspheres for a 3D mesenchymal stem cell culture

Cited by 18 publications

References 30 publications

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

3D Spheroid Cell Cultures and Their Role in Bone Regeneration: a Systematic Review

Preparation of thermosensitive PNIPAm‐based copolymer coated cytodex 3 microcarriers for efficient nonenzymatic cell harvesting during 3D culturing

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