Design and Construction of a Versatile Synthetic Network for Bistable Gene Expression in Mammalian Systems

Polynikis, A.; Cuccato, Giulia; Criscuolo, Stefania; Hogan, S. J.; Bernardo, Mario di; Bernardo, Diego di

doi:10.1089/cmb.2010.0208

Cited by 3 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The synthetic bistable switches constructed so far in vivo have not yet solved this dilemma. The host cells are typically forced on one state by exposition to strong inducer drugs for the whole switching time (7,13,43). Alternatively, nonmolecular stimuli, such as temperature or light, are used.…”

mentioning

confidence: 99%

Bottom-up construction of in vitro switchable memories

Padirac

Fujii

Rondelez

2012

Proc. Natl. Acad. Sci. U.S.A.

161

160

View full text Add to dashboard Cite

Reaction networks displaying bistability provide a chemical mechanism for long-term memory storage in cells, as exemplified by many epigenetic switches. These biological systems are not only bistable but switchable, in the sense that they can be flipped from one state to the other by application of specific molecular stimuli. We have reproduced such functions through the rational assembly of dynamic reaction networks based on basic DNA biochemistry. Rather than rewiring genetic systems as synthetic biology does in vivo, our strategy consists of building simplified dynamic analogs in vitro, in an artificial, well-controlled milieu. We report successively a bistable system, a two-input switchable memory element, and a single-input push-push memory circuit. These results suggest that it is possible to build complex time-responsive molecular circuits by following a modular approach to the design of dynamic in vitro behaviors. Our approach thus provides an unmatched opportunity to study topology/function relationships within dynamic reaction networks.

show abstract

mentioning

confidence: 99%

Bottom-up construction of in vitro switchable memories

Padirac

Fujii

Rondelez

2012

Proc. Natl. Acad. Sci. U.S.A.

161

160

View full text Add to dashboard Cite

show abstract

“…In a separate study, the aptamer was fused to a stabilizing stem-loop structure on the TetR aptamer, yielding an aptamer that could bind the tetracycline-dependent transactivator and inhibit expression of the reporter gene in HEK-293T and CHO-K1 cells . Similarly, a bistable switch controlled by theophylline and doxycycline demonstrated activity in HEK-293 and CHO cells …”

Section: Theophylline-sensing Riboswitchesmentioning

confidence: 99%

“…135 Similarly, a bistable switch controlled by theophylline and doxycycline demonstrated activity in HEK-293 and CHO cells. 136 Hsu et al used the aptamer to develop a ligand-specific −1 programmed ribosomal frameshift (−1 PRF) mechanism in HEK-293T cells. 137 The same group further expanded the system by inserting the aptamer into a stem of the SARS-PK pseudoknot and observed theophylline-dependent control of gene expression in vitro and in HEK-293T cells.…”

Section: ■ Theophylline Ribozymesmentioning

confidence: 99%

The Theophylline Aptamer: 25 Years as an Important Tool in Cellular Engineering Research

2020

View full text Add to dashboard Cite

The theophylline aptamer was isolated from an oligonucleotide library in 1994. Since that time, the aptamer has found wide utility, particularly in synthetic biology, cellular engineering, and diagnostic applications. The primary application of the theophylline aptamer is in the construction and characterization of synthetic riboswitches for regulation of gene expression. These riboswitches have been used to control cellular motility, regulate carbon metabolism, construct logic gates, screen for mutant enzymes, and control apoptosis. Other applications of the theophylline aptamer in cellular engineering include regulation of RNA interference and genome editing through CRISPR systems. Here we describe the uses of the theophylline aptamer for cellular engineering over the past 25 years. In so doing, we also highlight important synthetic biology applications to control gene expression in a ligand-dependent manner.

show abstract

Applications and modifications of aptamers: potential tool for medical microbiology

Fooladi

Hedayati

Amin

et al. 2016

Reviews in Medical Microbiology

View full text Add to dashboard Cite

Aptamers are ligands made of single-stranded DNA, oligonucleotide RNA or short peptides which bind specifically to their target molecules with high affinity. They resemble different types of monoclonal, polyclonal and recombinant antibodies in their mode of attachment. Aptamers optimally bind their targets in a range of μmol to ρmol. Targets vary from small molecules to macromolecules to whole cells. Systematic evolution of ligands by exponential enrichment is an affinity-based screening method for aptamers. Examples of aptamer applications include structural analysis of molecules, affinity/specificity testing, epitope mapping, making aptamer libraries by systematic evolution of ligands by exponential enrichment, pathogenic targeting, specific molecular targeting, live cell targeting, analytical applications, therapeutic potentials and drug delivery. Staphylococcal enterotoxin B is one of the most important bacterial toxins in different disorders. Aptamers offer a fast, feasible, reliable and affordable method for detecting this toxin.

show abstract

Design and Construction of a Versatile Synthetic Network for Bistable Gene Expression in Mammalian Systems

Cited by 3 publications

References 10 publications

Bottom-up construction of in vitro switchable memories

Bottom-up construction of in vitro switchable memories

The Theophylline Aptamer: 25 Years as an Important Tool in Cellular Engineering Research

Applications and modifications of aptamers: potential tool for medical microbiology

Contact Info

Product

Resources

About