Soft manipulators can perform continuous operations due to their inherent compliance and dexterity, thus enabling safe interactions and smooth movements in confined environments. However, high compliance usually means low load capacity. It is important for a soft manipulator to possess proper flexibility while maintaining an acceptable stiffness to widen its applications. This paper has hence devoted efforts to a kind of variable stiffness mechanism for a soft manipulator actuated by pneumatic artificial muscles (PAMs). Due to the combination of contractile and extensor PAMs, the manipulator is able to vary its stiffness independently from the configuration. The stiffness characteristics of the soft manipulator are quantitatively analyzed by bending shape prediction under different loading and inflation conditions, and the prediction is built upon a nonlinear statics model coupled with PAM nonlinearity and the Cosserat theory. In addition, experimental measurements are conducted to further validate the expected performance of the manipulator design. The experimental and verified theoretical analysis results indicate that the manipulator shape and stiffness are greatly affected by the pressure variation of PAMs, realizing a large bending space with a high output force. The variable stiffness design obviously increases the manipulator's ability to resist additional interference at the same position. INDEX TERMS Soft manipulator, PAM, bending shape prediction, variable stiffness, the Cosserat theory. LINA HAO received the Ph.D. degree in control theory and control engineering from Northeastern University, China, in 2001. From 2005 to 2006, she worked as a Visiting Researcher with Michigan State University, USA. She is currently a Professor with the School of Mechanical Engineering and Automation, Northeastern University. Her main research interests include design and control of micro-nano robotic systems, bionic drivers of artificial muscles, and smart sensors and actuators. She is a Fellow of the IEEE Robotics and Automation Society and the International Society of Bionic Engineering.