Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons

Pien, Nele; Peeters, Ian; Deconinck, Liesbet; Damme, Lana Van; Wilde, Lieven De; Martens, Ann; Vlierberghe, Sandra Van; Dubruel, Peter; Mignon, Arn

doi:10.1016/j.msec.2020.111504

Cited by 20 publications

(32 citation statements)

References 34 publications

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“…The idea of combining mechanical strength with a biological approach is a logical step. Therefore, in a prior study, a repair strategy that targets both of these aspects was developed 5 . A construct was designed that consists of an electrospun, acrylate-endcapped urethane-based precursor/poly(ε-caprolactone) (AUP/PCL) polymer that releases hyaluronic acid and naproxen.…”

Section: Accepted Articlementioning

confidence: 99%

“…Hyaluronic acid has been proven to promote gliding, while naproxen has the ability to counter adhesion formation around the repair complex 2,6 . The concept, choice of materials, characterization, biocompatibility, and mechanical ex vivo tests have been investigated and described in a previous publication 5 . However, the biological effects of the medicated polymers and the influence of the construct on vascularization of a tendon cannot be tested in an ex vivo setting.…”

Section: Accepted Articlementioning

confidence: 99%

“…In brief, an acrylate endcapped urethane-based precursor (AUP) material with a PCL backbone (MM 2000 g•mol -1 ) was synthesized, and together with PCL processed into an electrospun tube using an in-house developed electrospinning (ES) set-up (Fig. 1A) 5 . After optimization of the electrospinning parameters, a polymer ratio of 1:3 for AUP/PCL was selected.…”

Section: Design Of the Different Repair Constructsmentioning

confidence: 99%

“…In other words, an electrospun AUP/PCL-medicated polymer tube was used, consisting of two electrospun layers with naproxen and hyaluronic acid. This construct did not have sufficient intrinsic strength 5 and was therefore combined with a modified Kessler suture. Using this construct without reinforcement allows the evaluation of possible biological advantages of the AUP/PCL-medicated polymers without interference from the braid.…”

Section: Accepted Articlementioning

confidence: 99%

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Flexor tendon repair using a reinforced tubular, medicated electrospun construct

Peeters

Pien

Mignon

et al. 2021

Journal Orthopaedic Research

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A reinforced medicated electrospun construct for flexor tendon repair was investigated in a rabbit tendon model. The construct was compared to its individual components and to a modified Kessler suture. Biomechanical tensile testing, macroscopic and histological evaluation performed at week 3 and week 8 postoperatively, showed similar strength as a modified Kessler suture repair, a distinct pattern of adhesion, and good to excellent histologic repair. This study demonstrates that biologic and mechanical repair can successfully be combined.

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Section: Accepted Articlementioning

confidence: 99%

Section: Accepted Articlementioning

confidence: 99%

Section: Design Of the Different Repair Constructsmentioning

confidence: 99%

Section: Accepted Articlementioning

confidence: 99%

See 2 more Smart Citations

Flexor tendon repair using a reinforced tubular, medicated electrospun construct

Peeters

Pien

Mignon

et al. 2021

Journal Orthopaedic Research

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“…[ 148 ] Pien et al demonstrated that a three‐layered electrospinning scaffolds, whose inner layer was encapsulated anti‐inflammatory drug while its outer layer was coated with HA, showed great antiadhesion properties as tendon construct. [ 149 ] Collectively, electrospinning has an advantage of fabricating multipolymer/biomolecule solutions into hybrid scaffolds.…”

Section: Fabrication Of Polysaccharide‐based Scaffoldsmentioning

confidence: 99%

Engineering Polysaccharides for Tissue Repair and Regeneration

et al. 2021

Macromolecular Bioscience

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The success of repair or regeneration depends greatly on the architecture of 3D scaffolds that finely mimic natural extracellular matrix to support cell growth and assembly. Polysaccharides have excellent biocompatibility with intrinsic biological cues and they have been extensively investigated as scaffolds for tissue engineering and regenerative medicine (TERM). The physical and biochemical structures of natural polysaccharides, however, can barely meet all the requirements of tissue‐engineered scaffolds. To take advantage of their inherent properties, many innovative approaches including chemical, physical, or joint modifications have been employed to improve their properties. Recent advancement in molecular and material building technology facilitates the fabrication of advanced 3D structures with desirable properties. This review focuses on the latest progress of polysaccharide‐based scaffolds for TERM, especially those that construct advanced architectures for tissue regeneration.

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Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

Darroch

Asaro

Gréant

et al. 2023

J Biomedical Materials Res

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The use of polymeric biomaterials to create tissue scaffolds using additive manufacturing techniques is a well‐established practice, owing to the incredible rapidity and complexity in design that modern 3D printing methods can provide. One frontier approach is melt electrowriting (MEW), a technique that takes advantage of electrohydrodynamic phenomena to produce fibers on the scale of 10's of microns with designs capable of high resolution and accuracy. Poly(ε‐caprolactone) (PCL) is a material that is commonly used in MEW due to its favorable thermal properties, high stability, and biocompatibility. However, one of the drawbacks of this material is that it lacks the necessary chemical groups which allow covalent crosslinking of additional elements onto its structure. Attempts to functionalise PCL structures therefore often rely on the functional units to be applied externally via coatings or integrally mixed elements. Both can be extremely useful depending on their applications, but can add extra difficulties into the use of the resulting structures. Coatings require careful design and application to prevent rapid degradation, while elements mixed into the polymer melt must deal with the possibilities of phase separation and changes to MEW properties of the unadulterated polymer. With this in mind, this study sought to imbibe functionality to MEW‐printed scaffolds using the approach of adding functional units directly, via covalent bonding of functional groups to the polymer itself. To this end, this study employs a recently developed class of polymers called acrylate‐endcapped urethane‐based polymers (AUPs). The polymer backbone of the specific AUP used consists of a poly(D,L‐lactic acid) (PDLLA)/PCL copolymer chain, which is functionalized with 6 acrylate groups, 3 at either end. Through blending of the AUP with PCL, various concentrations of this mixture were used with MEW to produce scaffolds that possessed acrylate groups on their surface. Using UV crosslinking, these groups were tagged with Fluorescein‐o‐Acrylate to verify that PDLLA/PCL AUP/PCL blends facilitate the direct covalent bonding of external agents directly onto the MEW material. Blending of the AUP with PCL increases the scaffold's stiffness and ultimate strength. Finally, blends were proven to be highly biocompatible, with cells attaching and proliferating readily at day 3 and 7 post seeding. Through this work, PDLLA/PCL AUP/PCL blends clearly demonstrated as a biocompatible material that can be processed using MEW to create functionalised tissue scaffolds.

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Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons

Cited by 20 publications

References 34 publications

Flexor tendon repair using a reinforced tubular, medicated electrospun construct

Flexor tendon repair using a reinforced tubular, medicated electrospun construct

Engineering Polysaccharides for Tissue Repair and Regeneration

Melt electrowriting of a biocompatible photo‐crosslinkable poly(D,L‐lactic acid)/poly(ε‐caprolactone)‐based material with tunable mechanical and functionalization properties

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