Patient transfer has always been a difficult problem in the hospital. For medical staff, there are problems including high risk of infection, heavy physical labor and low efficiency of transfer; for patients, there are problems including poor comfort and secondary injury. In this paper, a novel bilateral patient transfer robot is investigated and designed. The following tasks are conducted: (1) Based on the process of patient transfer, a transfer model, which consists of two degrees of freedom, is proposed, and the working principle of bilateral patient transfer robot is obtained and analyzed in detail. (2) Force analysis of the patient transfer robot is conducted. The corresponding relationship between the patient comfort and the insertion angle is proposed, and the optimal sizes of mechanical structure are obtained. (3) Based on the theoretical analysis, the mechanical structure and the control system of the robot are designed, and the prototype is manufactured. (4) Experimental research is conducted. The results show that the prototype can complete the required motion performance with a carrying capacity up to 150 kg and patient comfort is excellent. The results of this paper prove that this kind of patient transfer robot has good performance, it can also reduce the burden on medical staff.