Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy

Tabesh, Saeideh; Fanuel, Songwe; Fazlollahi, Mohammad Reza; Yekaninejad, Mir Saeed; Kardar, Gholam Ali; Razavi, Seyed Alireza

doi:10.1016/j.intimp.2018.10.037

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…A hypoallergenic vaccine based on B-cell epitopes was designed and called Sal k 1-KLH, composed of a peptide Current Developments in Allergen-Specific Immunotherapy: A Brief Review DOI: http://dx.doi.org/10.5772/intechopen.106280 derived from the allergen Sal k 1 conjugated to the keyhole limpet hemocyanin transporter molecule. This study showed that the vaccine produced high IgG levels in immunized mice that block IgE interaction but did not show a T cell lymphocyte response compared to the extract and the recombinant [33].…”

Section: Carrier-bound B-cell Epitope-containing Peptidesmentioning

confidence: 86%

Current Developments in Allergen-Specific Immunotherapy: A Brief Review

Bartholazzi¹,

Lodi²,

Machado³

2023

Allergic Disease - New Developments in Diagnosis and Therapy

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Immunotherapy is a treatment for patients with type I-mediated allergic diseases. Molecular forms of allergen-specific immunotherapy (AIT), based on inducing immunological tolerance characterized by increased IL-10, TGF-β, and IgG4 levels, and Treg cell are continuously emerging to improve the efficacy of the treatment, shorten the duration of protocols, and prevent any side effects. This review covers the recent progress in AIT and routes of antigen administration. Classical immunotherapy uses allergen extracts obtained from natural sources. Limitations of the uses of these extracts, such as sensitizations with nonspecific agents, can be avoided using purified components, hypoallergenic recombinant proteins, and vaccines based on peptides (epitopes). However, these molecules have low immunogenicity requiring new carriers or more effective adjuvants. Vaccines based on carrier-bound B-cell epitope-containing peptides and the constructions of allergens coupled to virus-like particles (VLPs) are under evaluation. The possibility of vaccinating with DNA encoding the allergen to obtain an allergen-specific Th1 and IgG response is in development and the success of messenger ribonucleic acid (mRNA) vaccines against severe acute respiratory syndrome Coronavirus 2 must encourage as well the re-exploration of mRNA vaccine platform for innovative AIT.

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Section: Carrier-bound B-cell Epitope-containing Peptidesmentioning

confidence: 86%

Current Developments in Allergen-Specific Immunotherapy: A Brief Review

Bartholazzi¹,

Lodi²,

Machado³

2023

Allergic Disease - New Developments in Diagnosis and Therapy

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“…Avoiding contact with food allergies is the most common and difficult way for allergic patients to control food allergy. Based on the comprehensive cognition of epitopes, hypoallergenic derivatives could be obtained by modifying or molecularly modifying dominant epitopes to mask or mutate these, and safe and effective immunotherapy for food allergy could be established. ,, In addition, some nonconservation epitopes had been found to serve as allergen detection biomarkers, avoiding excessive prevention and control of allergen related foods and bringing convenience to patients and their families . As was well-known, the physical and chemical properties of amino acids determine the structure and functional behavior of proteins .…”

Section: Discussionmentioning

confidence: 99%

Immunoglobulin E Epitope Mapping and Structure–Allergenicity Relationship Analysis of Crab Allergen Scy p 9

He,

Yang,

Chen

et al. 2023

J. Agric. Food Chem.

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Filamin C is an allergen of Scylla paramamosain (Scy p 9), and six IgE linear epitopes of the allergenic predominant region had previously been validated. However, the IgE epitope and structure–allergenicity relationship of Scy p 9 are unclear. In this study, a hydrophobic bond was found to be an important factor of conformation maintaining. The critical amino acids in the six predicted conformational epitopes were mutated, and the IgE-binding capacity and surface hydrophobicity of four mutants (E216A, T270A, Y699A, and V704A) were reduced compared to Scy p 9. Ten linear epitopes were verified with synthetic peptides, among which L-AA187–205 had the strongest IgE-binding capacity. In addition, IgE epitopes were mapped in the protruding surface of the tertiary structure, which were conducive to binding with IgE and exhibited high conservation among filamin genes. Overall, these data provided a basis for IgE epitope mapping and structure–allergenicity relationship of Scy p 9.

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“…B cell epitopes identified and validated by ELISA could be chemically coupled to a carrier protein or be produced as recombinant fusion proteins and administered to achieve these objectives. One classical protein carrier is the keyhole limpet hemocyanin, which induces allergen-specific IgG against allergens [38]. Moreover, the inclusion of peptides from the IgE-binding sites would focus allergenspecific IgG responses to the β-PRVB IgE-epitopes, potentially resulting in better protection and a reduced number of vaccine administrations.…”

Section: Introductionmentioning

confidence: 99%

Proteomics for Development of Food Allergy Vaccines

Carrera

Magadán

2021

Methods in Molecular Biology

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Food allergy is a hypersensitivity reaction to food products initiated by immunologic mechanisms, which represents one of the major concerns in food safety. New therapies for food allergies including oral and epicutaneous allergen-specific immunotherapy are required, and B cell epitope-based allergy vaccines are a good promise to improve this field. In this chapter, we describe a workflow for the design of food allergy vaccines using proteomic tools. The strategy is defined based on the characterization of B cell epitopes for a particular food allergen. For that, the workflow comprises five consecutive steps: (1) shotgun proteomics analysis of different protein isoforms for a particular food allergen, (2) downloading all protein sequences for the specific allergen included in UniProtKB database, (3) analysis by protein-based bioinformatics of B cell epitopes, (4) synthesizing of the selected B cell peptide epitopes, and (5) performing of immunoassays using sera from healthy and allergic patients. The results from this method provide a rationale repository of B cell epitopes for the design of new specific immunotherapies for a particular food allergen. The strategy was optimized for all the beta-parvalbumins (β-PRVBs), which are considered as the main fish allergens. Using this workflow, a total of 35 peptides were identified as B cell epitopes, among them the top 4 B cell peptide epitopes that may induce protective immune response were selected as potential peptide vaccine candidates for fish allergy.

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Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy

Cited by 7 publications

References 38 publications

Current Developments in Allergen-Specific Immunotherapy: A Brief Review

Current Developments in Allergen-Specific Immunotherapy: A Brief Review

Immunoglobulin E Epitope Mapping and Structure–Allergenicity Relationship Analysis of Crab Allergen Scy p 9

Proteomics for Development of Food Allergy Vaccines

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