Design and evaluation of D-α tocopheryl polyethylene glycol 1000 succinate emulsified poly-ϵ-caprolactone nanoparticles for protein/peptide drug delivery

Rayaprolu, Bindhu Madhavi; Strom, James G.

doi:10.3109/03639045.2012.699069

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…Herein we investigate the feasibility of creating LPNs containing PCL and PC:glyceryl tripalmitate mixture as the polymer and lipid constituents, respectively. The PCL is one of the most widely used biodegradable polyester in the preparation of nanoparticles due to its great biocompatibility and biodegradability properties [14]. However, it is not water soluble and this poses a significant challenge to encapsulating hydrophilic drugs into water-insoluble polymers efficiently.…”

Section: Resultsmentioning

confidence: 99%

“…PCL is a biocompatible and biodegradable polymer which is non-toxic and has great permeability to several drugs. Degradation of PCL does not result in an acidic environment which might disrupt the structure and properties of proteins, unlike the other commonly used biodegradable polymers such as poly(lactide) (PLA) and poly (lactide-co-glycolide) (PLGA) [14].…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of Protein-loaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core Material

Devrim¹,

BozkÄ±r²

2014

J Biomol Res Ther

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Lipid-polymer hybrid nanoparticles (LPNs) have emerged as a potent therapeutic nano-carrier alternative to liposomes and polymeric nanoparticles. In this work, lipid-polymer hybrid nanoparticles were prepared using polycaprolactone, phosphatidylcholine: glyceryl tripalmitate mixture and lysozyme as the polymer, lipids and model protein, respectively. Uniform nanoparticles with about 100 nm in size were obtained using the modified emulsification solvent evaporation method. The results indicated that LPNs showed higher encapsulation efficiency compared with naked polycaprolactone nanoparticles. According to the results of bioactivity assay, 63.86% bioactive lysozyme was recovered from the LPNs. These results indicated that modification of polycaprolactone nanoparticles with lipids could considerably increase the drug-delivery efficiency and LPNs had potential in the delivery of peptides and proteins.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Protein-loaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core Material

Devrim¹,

BozkÄ±r²

2014

J Biomol Res Ther

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“…LPNs containing HIP complex of lysozyme with SDS were prepared by s/o/w emulsification-solvent-evaporation method. PCL was chosen as the polymeric core material due to its great biodegradability and biocompatibility [20]; phosphatidylcholine (PC) and glyceryl tripalmitate were selected as lipids. Furthermore, lysozyme-loaded LPNs were prepared to compare with complex-loaded LPNs.…”

Section: Preparation and Characterization Of Hip Complex-loaded Lpnsmentioning

confidence: 99%

Design and Evaluation of Hydrophobic Ion-Pairing Complexation of Lysozyme with Sodium Dodecyl Sulfate for Improved Encapsulation of Hydrophilic Peptides/Proteins by Lipid-Polymer Hybrid Nanoparticles

Bozkır¹

2015

J Nanomed Nanotechnol

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Hydrophobic ion-pairing (HIP) complexation-based approach has been employed to reduce aqueous solubilities of peptide and protein drugs. The solubility of a protein molecule is due to presence of charged amino acids present on the surface. HIP complexation is a technique to complex ionizable functional groups of protein and peptide molecules with oppositely charged functional groups of a complex forming agent. The main objective of this study was to formulate and evaluate HIP complexes of lysozyme with sodium dodecyl sulfate (SDS) as an ion paring agent. Results of % binding effciency shown that the formation of HIP complexes were dependent on pH of lysozyme solution and molar ratio of lysozyme to SDS. Aqueous solubilities of HIP complexes were very low compared to lysozyme alone. The e¬ffect of HIP complexation on enzymatic activity of lysozyme was also studied. Further, lipid-polymer hybrid nanoparticles (LPNs) loaded with lysozyme and lysozyme:SDS HIP complex were prepared by using a solid-in-oil-in-water (s/o/w) emulsion solvent evaporation method and characterised with respect to morphology, size and encapsulation efficiency. We observed significant improvement in encapsulation efficiency for lysozyme:SDS HIP complex-loaded LPNs. This study demonstrates a novel approach of formulating protein-loaded nanoparticles which can also be employed for delivery of proteins.

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“…This makes them interesting in food industry for nutriment encapsulation like magnesium, vitamins, , probiotics, or ω3- or n3-fatty acid prone to oxidation. − An interesting application also consists of “low fat content″ , emulsions, since a part of the oil is replaced by water without texture modification. In the pharmaceutical industry, double emulsions are often used as final products to encapsulate various drugs of interest as peptides, − hormones, steroids, or antiseptic or as a way to produce microspheres. − Microspheres or microcapsules are produced by evaporation of the intermediate phase thus requiring the use of a solvent. Double emulsions also exhibit the possibility to simultaneously encapsulate both oil and water compounds.…”

Section: Introductionmentioning

confidence: 99%

Determination of Formulation Conditions Allowing Double Emulsions Stabilized by PGPR and Sodium Caseinate to Be Used as Capsules

et al. 2018

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Water-in-oil-in-water (W/O/W) double emulsions stabilized by polyglycerol polyricinoleate (PGPR), a lipophilic food grade small polymer, and sodium caseinate, a hydrophilic milk protein, were developed to encapsulate vitamin B12, a model hydrophilic substance easy to titrate. Using rheology, sensitive to drop size evolution and water fluxes, static light scattering, and microscopy both giving the evolution of drops' size and vitamin B12 titration assessing the encapsulation, we were able to detect independently the double emulsion drop size, the encapsulation loss, and the flux of water as a function of time. By differentiating the PGPR required to cover the W-droplets' surface from PGPR in excess in the oil phase, we built a PGPR-inner droplet volume fraction diagram highlighting the domains where the double emulsion is stable toward encapsulation and/or water fluxes. We demonstrated the key role played by nonadsorbed PGPR concentration in the intermediate sunflower oil phase on the emulsion stability while, surprisingly, the inner droplet volume fraction had no effect on the emulsion stability. At low PGPR concentration, a release of vitamin B12 was observed and the leakage mechanism of coalescence between droplets and oil-water interface of the oily drops (also called globules hereafter), was identified using confocal microscopy. For high enough PGPR content, the emulsions were stable and may therefore serve as efficient capsules without need of an additional gelling, thickening, complexion or interface rigidifying agent. We generalized these results with the encapsulation of an insecticide: Cydia pomonella granulovirus used in organic arboriculture.

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Design and evaluation of D-α tocopheryl polyethylene glycol 1000 succinate emulsified poly-ϵ-caprolactone nanoparticles for protein/peptide drug delivery

Abstract: Based on the results obtained, these TPGS-emulsified PCL nanoparticles proved to be potential carriers for the delivery of protein/peptide drugs.

Cited by 10 publications

References 29 publications

Preparation and Characterization of Protein-loaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core Material

Preparation and Characterization of Protein-loaded Lipid-polymer Hybrid Nanoparticles with Polycaprolactone as Polymeric Core Material

Design and Evaluation of Hydrophobic Ion-Pairing Complexation of Lysozyme with Sodium Dodecyl Sulfate for Improved Encapsulation of Hydrophilic Peptides/Proteins by Lipid-Polymer Hybrid Nanoparticles

Determination of Formulation Conditions Allowing Double Emulsions Stabilized by PGPR and Sodium Caseinate to Be Used as Capsules

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