Design and Evaluation of Modified Chitosan Based in Situ Gel for Ocular Drug Delivery

Kadam, Anuja T.; Jadhav, Rahul Laxman; Salunke, Pradnya B.; S, Kadam Satwashila

doi:10.22159/ijpps.2017v9i11.20938

Cited by 13 publications

(10 citation statements)

References 11 publications

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“…Modified chitosan at different concentrations did not influence the gelation temperature, but enabled the formation of viscous solutions. The association with modified chitosan resulted in increased mucoadhesiveness and prolonged erosion time for the analyzed preparations [69].…”

Section: Strategies To Increase Residence Time On the Ocular Surfacementioning

confidence: 99%

Strategies for Improving Ocular Drug Bioavailability and Corneal Wound Healing with Chitosan-Based Delivery Systems

et al. 2018

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The main inconvenience of conventional eye drops is the rapid washout of the drugs due to nasolacrimal drainage or ophthalmic barriers. The ocular drug bioavailability can be improved by either prolonging retention time in the cul-de-sac or by increasing the ocular permeability. The focus of this review is to highlight some chitosan-based drug delivery approaches that proved to have good clinical efficacy and high potential for use in ophthalmology. They are exemplified by recent studies exploring in-depth the techniques and mechanisms in order to improve ocular bioavailability of the active substances. Used alone or in combination with other compounds with synergistic action, chitosan enables ocular retention time and corneal permeability. Associated with other stimuli-responsive polymers, it enhances the mechanical strength of the gels. Chitosan and its derivatives increase drug permeability through the cornea by temporarily opening tight junctions between epithelial cells. Different types of chitosan-based colloidal systems have the potential to overcome the ocular barriers without disturbing the vision process. Chitosan also plays a key role in improving corneal wound healing by stimulating the migration of keratinocytes when it is used alone or in combination with other compounds with synergistic action.

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Section: Strategies To Increase Residence Time On the Ocular Surfacementioning

confidence: 99%

Strategies for Improving Ocular Drug Bioavailability and Corneal Wound Healing with Chitosan-Based Delivery Systems

et al. 2018

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“…The minimum inhibitory concentration (MIC) was determined by the following CLSI procedure for the broth microdilution method [35]. The bacterial lines have been cultured onto Luria Bertani Agar medium (containing 0.5% peptone, 0.5% yeast extract, 1% NaCl and 1.5% Agar, at pH 7.5±0.2).…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Novel Sa-Pa-Lsa/C-30b/Ag Nanocomposites for Swelling, Antibacterial, Drug Delivery, and Anticancer Applications

Rauta

Venkatalakshimi³

et al. 2018

Asian J Pharm Clin Res

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Objective: The main objective of this work was to formulate and evaluate Closite-30B/nanoAg filled hydrogel composites which are further intentended to be used for the study of drug delivery,antibacterial, and anticancer activityMethods: In this study, Cloisite-30B (C-30B) clay dispersed biopolymer sodium alginate (SA)-grafted-poly (acrylamide [AAm]-co-lignosulfonic acid) hydrogel composites were synthesized by free radical in situ polymerization reaction technique using SA, AAm, and lignosulfonic acid biopolymers in different proportions in combination. which are subjected to invitro drug delivery and Minimum inhibitory concentration(MIC) method for antibacterial activity study by using Streptococcus faecalis (S.faecalis) and Escherichia coli (E. coli)bacteria. The biocompatibility of the prepared gels were determined by standard protocol HaCaT-cells and MCF-7 cell lines further the prepared hydrogel composites were characterized for particle size,encapsulation efficiency,swelling properties,compatibility studies by FTIR etc.Results: The formulated hydrogels were characterized by X-ray diffraction (XRD) to analyze the particles size and crystallinity. The presence of functional groups and their chemical interaction with the drug, C-30B, and silver nanoparticles (AgNPs) were confirmed by the FTIR spectroscopy. Furthermore, the presence of AgNPs in the matrix was confirmed by ultraviolet/visible spectroscopy. Thermogravimetric analysis was performed to find out the thermal degradation, thermal stability, and the percentage of weight loss at various temperatures. Swelling studies revealed that C-30B and AgNPs induced composites exhibited higher swelling ratio than pure hydrogels. The hydrogels with C-30B/AgNPs displayed excellent antibacterial activity against both Gram-positive and Gram-negative bacteria. Further, these hydrogel composites were loaded with the drug paclitaxel (PT), and drug release study showed that the sustained release of the drug from C-30B/Ag hydrogel matrix compared to rest of other samples. Hydrogel composites were cytocompatible in nature (with HaCaT cells) and the cell viability decreased (with MCF-7cells) with the presence of lignosulfonic acid as well as C-30B and AgNPs in the samples as evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide to its insoluble formazan assay.Conclusion: The synthesized hydrogel composites were successfully characterized and eavaluated for sustained release of paclitaxel drug delivery at different pHs and temperatures and it is found that C30B/Ag filled composites exhibits contolled release of drug with higher rate, especially at lower pH (pH2) and higher temperature (37oC) and the same formulations which exhibits better anitbcterial and anticancer activity compared to the virgin samples So the prepared C30B/AgNPs hydrogels composites used in drug dlivery for the effective treatment of cancer and used against bacterias and cancerous cells.

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“…Some researchers interpret gelling capacity differently, such as (-) No gelation; (+) Gels after some min and breaks up quickly; (++) Gelation immediately then lasts for several h; (+++) Gelation immediately thereafter remains for an extended period; (++++) Stiff gel in studies [12,20,25,28,29], or (+) gel is shaped in>40 s and melts in 1-2 min;…”

Section: Methodsmentioning

confidence: 99%

A Review on How to Characterize and Evaluate the Ophthalmic in Situ Gel Preparations

et al. 2022

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In situ gelling systems are becoming one of the most popular and well-known, with many potential benefits from delivery systems, such as ease of use and ease of manufacture, improve adherence and patient comfort by minimizing the frequency of drug administration. In this review, we will describe the characterization and evaluation of the ophthalmic in situ gel preparation. Among them are physical evaluation (appearance and clarity, pH, isotonicity, gelation temperature, gelling capacity, viscosity, and stability), chemical evaluation (determining drug content, drug release), microbiological evaluation (sterility, ocular irritability, ocular tolerability, antimicrobial activity, hemolysis activity, bovine corneal opacity and permeability (BCOP) test, preservative efficacy test (PET), microtetrazolium (MTT) reduction cytotoxicity test), and in vivo evaluation such as pharmacokinetic and pharmacodynamic evaluation. Characterizing the chemical, physical, microbiological, and miscellaneous properties of ophthalmic in situ gel formulations can meet the ideal requirements and help determine the best formulation of ophthalmic in situ gel to achieve higher bioavailability values, longer contact times, minimize side effects, not causing irritation or liquid tear production, and providing a maximum therapeutic effect. In situ gels offer the primary requirement of a successful controlled release product that is increasing patient compliance.

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Design and Evaluation of Modified Chitosan Based in Situ Gel for Ocular Drug Delivery

Cited by 13 publications

References 11 publications

Strategies for Improving Ocular Drug Bioavailability and Corneal Wound Healing with Chitosan-Based Delivery Systems

Strategies for Improving Ocular Drug Bioavailability and Corneal Wound Healing with Chitosan-Based Delivery Systems

Synthesis and Characterization of Novel Sa-Pa-Lsa/C-30b/Ag Nanocomposites for Swelling, Antibacterial, Drug Delivery, and Anticancer Applications

A Review on How to Characterize and Evaluate the Ophthalmic in Situ Gel Preparations

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