Design and evaluation of scFv-RTX-A as a novel immunotoxin for breast cancer treatment: an in silico approach

Tehrani, Sadra Samavarchi; Gharibi, Saba; Movahedpour, Ahmad; Goodarzi, Golnaz; Jamali, Zeinab; Khatami, Seyyed Hossein; Maniati, Mahmood; Ranjbar, Maryam; Shabaninejad, Zahra; Taheri‐Anganeh, Mortaza

doi:10.1080/15321819.2020.1812640

Cited by 14 publications

(7 citation statements)

References 57 publications

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“…There is a significant difference in the survival rate of BC worldwide. That is, it is estimated the five‐year survival rate of breast cancer in developed countries is 80% whereas in developing countries, the rate is below 40% 3,4 . According to the presence or absence of molecular markers for oestrogen or progesterone receptors and human epidermal growth factor 2 (ERBB2, formerly HER2), 5,6 BC is divided into three major subtypes: hormone receptor positive/ ERBB2 negative (70% of patients), ERBB2 positive (15‐20%) and triple negative (tumours that do not have all three markers, 15%) 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Gene therapy: A promising approach for breast cancer treatment

Dastjerd

Valibeik

Monfared

et al. 2021

Cell Biochemistry & Function

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Breast cancer (BC) is the most prevalent malignancy and the second leading cause of death among women worldwide that is caused by numerous genetic and environmental factors. Hence, effective treatment for this type of cancer requires new therapeutic approaches. The traditional methods for treating this cancer have side effects, therefore so much research have been performed in last decade to find new methods to alleviate these problems. The study of the molecular basis of breast cancer has led to the introduction of gene therapy as an effective therapeutic approach for this cancer. Gene therapy involves sending genetic material through a vector into target cells, which is followed by a correction, addition, or suppression of the gene. In this technique, it is necessary to target tumour cells without affecting normal cells. In addition, clinical trial studies have shown that this approach is less toxic than traditional therapies. This study will review various aspects of breast cancer, gene therapy strategies, limitations, challenges and recent studies in this area.

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Section: Introductionmentioning

confidence: 99%

Gene therapy: A promising approach for breast cancer treatment

Dastjerd

Valibeik

Monfared

et al. 2021

Cell Biochemistry & Function

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“…The Food and Drug Administration (FDA) has approved several drugs for BC treatment including pertuzumab, lapatinib and trastuzumab. However, these drugs show draw backs including their large molecular size and high immunogenicity [33]. While a conjugation of a cytokine (IFN-β) to the small chain fragment (ScFv) of an antibody increases the e cacy, speci city and potency of anti-EGFR antibody [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…Chan Gyu Lee et al, used IFN-β in combination with trastuzumab and evaluated its expression in cell lines that shown satisfactory results [39]. Asma vafadar et al designed ScFv and conjugated it with a toxin (Cj-CdtB) and concluded ScFv as a stable and soluble protein with strong binding a nity with HER2 receptor [40]. Another study conducted by Parnian Navabi et al, also designed immunocytokine containing ScFv as a conjugate molecule along with IL-2Rα protein and reported its e cient binding a nity with HER2 receptor with successful production and functionality in E. coli Bl21 (DE3) periplasmic space, following molecular docking [41].…”

Section: Discussionmentioning

confidence: 99%

In silico construction and evaluation of anti-EGFR ScFv and IFN-β fusion protein as a novel immunocytokine for the treatment of HER1 positive breast cancer

Khan

Hassan

Sohail

et al. 2023

Preprint

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Context Human epidermal growth factor receptor 1 (HER1) positive breast cancer is characterized by overexpression of the HER1 receptor protein on cancer cells leading to aggressive disease and treatment resistance. Computational design can efficiently create immunocytokine that specifically target cancer cell antigens and modulate the immune response, making them a promising treatment for HER1-positive breast cancer. Methods The current work involves computational tools to design a novel immunocytokine, including the anti-epidermal growth factor single-chain variable fragment (ScFv) against HER1 receptor with a connection to the functional part of a cytokine INF-β. The amino acid sequences of ScFv region of antibody and IFN-β were retrieved from gene bank (accession no. AFF61391.1) and (ABS89222.1) respectively, and fused together to form a chimeric protein, called ScFv-IFN-β fusion protein. Physicochemical properties and solubility were analyzed using ProtParam and SOLpro, respectively. The ScFv-IFN-β fusion protein was modeled in 3D using alpha-fold and refined with GalaxyRefine and assessed using PROCHECK and RAMPAGE. Allergenicity and mRNA stability were predicted using the AlgPred and RNAfold servers, respectively. Docking and molecular dynamic simulations were performed using HDOCK and iMOD servers, respectively. Our computational analysis predicted that the ScFv-IFN-β fusion protein is anticipated to exhibit solubility, possess a sturdy 3D structure, maintain stable mRNA structure, and effectively bind to both HER1 and IFN receptors, thus making it a suitable candidate for breast cancer treatment. The current findings appear encouraging and hold promise for future experimental investigations.

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“…coli is a general and simple host for the expression of recombinant proteins, which is appropriate for the production of various valuable proteins such as vaccines, immunotoxins, and biopharmaceuticals. [111][112][113][114][115][116][117][118][119][120] Heterologous expression in E. coli is often used to solve the problem of laccase production that is not readily available from natural sources. A set of laccases is expressed in bacterial hosts.…”

Section: Bacterial Recombinant Systemsmentioning

confidence: 99%

Laccase: Various types and applications

Khatami

Vakili

Movahedpour³

et al. 2022

Biotech and App Biochem

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Laccase belongs to the polyphenol oxidase family and is very important in removing environmental pollutants due to its structural and functional properties. Recently, the ability of laccase to oxidize phenolic and nonphenolic substances has been considered by many researchers. This enzyme's application scope includes a broad range of chemical processes and industrial usages, such as bioremediation, nanobiotechnology, woodworking industries, bleaching of paper pulp, dyeing in the textile industry, biotechnological uses in food industries, biorefining, detoxification from wastewater, production of organic matter from phenolic and amine substrates, and biofuels. Although filamentous fungi produce large amounts of laccase, high-yield industrial-scale production of laccase is still faced with many problems. At present, researchers are trying to increase the efficiency and productivity and reduce the final price of laccase by finding suitable microorganisms and improving the process of production and purification of laccase. This article reviews the introduction of laccase, its properties, production processes, and the effect of various factors on the enzyme's stability and activity, and some of its applications in various industries.

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Design and evaluation of scFv-RTX-A as a novel immunotoxin for breast cancer treatment: an in silico approach

Cited by 14 publications

References 57 publications

Gene therapy: A promising approach for breast cancer treatment

Gene therapy: A promising approach for breast cancer treatment

In silico construction and evaluation of anti-EGFR ScFv and IFN-β fusion protein as a novel immunocytokine for the treatment of HER1 positive breast cancer

Laccase: Various types and applications

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