Considering the problems of the low mechanical work rate and the high picking damage rate of baffle roller bionic picking devices in the harvesting process of fresh corn in China, a method of fresh corn harvesting based on dislocation baffle roller bionic picking is proposed. When the picking device is in operation, the dislocation baffle roller assists with picking by applying deflecting torque to the corn cob. The mechanical properties of the bottom kernel were significantly better than those of the top kernel, according to the results of a triaxial compression test on fresh corn kernels, and the force applied by the picking device from the bottom kernel’s side could successfully prevent cob breakage. To determine the optimal combination of operating parameters for the bionic picking device, a three-factor, three-level virtual response surface optimization test was conducted using Box–Behnken’s central combination method with the baffle roller tilt angle, the baffle roller gap, and the stalk speed as the test factors, and the maximum contact force as the test indexes. Based on the theoretical analysis results, a test bench of the dislocation baffle roller bionic picking device was made. When the picking damage rate was 0.32%, the baffle roller dislocation was 5 mm, the baffle roller tilt angle was 41°, the baffle roller gap was 25 mm, and the stalk speed was 338 mm/s. This study can provide a reference for researching low-damage picking of fresh corn cob.