Design and fabrication of bio-hybrid materials using inkjet printing

Maddaus, Alec G.; Curley, Patrick; Griswold, Matthew A.; Costa, Bianca Daniela; Hou, Steven X.; Jeong, Kwang‐Yong; Song, Edward; Deravi, Leila F.

doi:10.1116/1.4966164

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…Particularly, due to its on-demand printability of the devices, inkjet-printed sensors can potentially be used as point-of-care (POC) diagnostic tools and disposable testing kits. In contrast to other existing aptamer immobilization techniques, the proposed approach of inkjet-printing offers many advantages, including mass producibility, uniform deposition of materials, fully automated process, and high throughput [ 22 , 23 , 24 ]. We also demonstrate in this work that the aptamer density can be controlled by utilizing the number of printing layers.…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme

2018

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Recently, inkjet-printing has gained increased popularity in applications such as flexible electronics and disposable sensors, as well as in wearable sensors because of its multifarious advantages. This work presents a novel, low-cost immobilization technique using inkjet-printing for the development of an aptamer-based biosensor for the detection of lysozyme, an important biomarker in various disease diagnosis. The strong affinity between the carbon nanotube (CNT) and the single-stranded DNA is exploited to immobilize the aptamers onto the working electrode by printing the ink containing the dispersion of CNT-aptamer complex. The inkjet-printing method enables aptamer density control, as well as high resolution patternability. Our developed sensor shows a detection limit of 90 ng/mL with high target selectivity against other proteins. The sensor also demonstrates a shelf-life for a reasonable period. This technology has potential for applications in developing low-cost point-of-care diagnostic testing kits for home healthcare.

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Section: Introductionmentioning

confidence: 99%

A Low-Cost Inkjet-Printed Aptamer-Based Electrochemical Biosensor for the Selective Detection of Lysozyme

2018

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“…Proliferation of hDFs on the porous hydrogel was higher than on the nonporous hydrogel over the two week period, and there was no statistical significance between the two groups at week 2. Various studies have shown that mTG-cross-linked nonporous gelatin hydrogel supports cell adhesion and proliferation. , The fact that the macroporous gelatin hydrogel resulted in a higher cellular proliferation than the nonporous gelatin hydrogel proves its excellent bioactivity properties and its potential use in biological systems such as for wound healing. The excellent hDF proliferation on the gelatin macroporous hydrogel is comparable to the PEG-based macroporous hydrogel, which also supported robust proliferation of hDFs encapsulated in the hydrogel .…”

Section: Resultsmentioning

confidence: 99%

Injectable Macroporous Hydrogel Formed by Enzymatic Cross-Linking of Gelatin Microgels

Hou

Lake

Park

et al. 2018

ACS Appl. Bio Mater.

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Injectable hydrogels can be useful tools for facilitating wound healing since they conform to the irregular shapes of wounds, serving as a temporary matrix during the healing process. However, the lack of inherent pore structures of most injectable hydrogels prohibits desired interactions with the cells of the surrounding tissues limiting their clinical efficacy. Here, we introduce a simple, cost-effective and highly biofunctional injectable macroporous hydrogel made of gelatin microgels crosslinked by microbial transglutaminase (mTG). Pores are created by the interstitial space among the microgels. A water-in-oil emulsion technique was used to create gelatin microgels of an average size of 250μm in diameter. When crosslinked with mTG, the microgels adhered to each other to form a bulk hydrogel with inherent pores large enough for cell migration. The viscoelastic properties of the porous hydrogel were similar to those of nonporous gelatin hydrogel made by adding mTG to a homogeneous gelatin solution. The porous hydrogel supported higher cellular proliferation of human dermal fibroblasts (hDFs) than the nonporous hydrogel over two weeks, and allowed the migration of hDFs into the pores. Conversely, the hDFs were unable to permeate the surface of the nonporous hydrogel. To demonstrate its potential use in wound healing, the gelatin microgels were injected with mTG into a cut out section of an excised porcine cornea. Due to the action of mTG, the porous hydrogel stably adhered to the cornea tissue for two weeks. Confocal images showed that a large number of cells from the cornea tissue migrated into the interstitial space of the porous hydrogel. The porous hydrogel was also used for the controlled release of platelet-derived growth factor (PDGF), increasing the proliferation of hDFs compared to the nonporous hydrogel. This gelatin microgel-based porous hydrogel will be a useful tool for wound healing and tissue engineering.

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“…[3][4][5] A variety of materials, including organics, polymers, metals, hybrids and oxides can be deposited using inkjet printing and the technique conserves costly processing materials. [5][6][7][8][9][10][11] Inkjet printing technology has been in widespread use since the 1970s, primarily for printing pigment based inks on paper. 12 These printers have evolved from the continuous ink dispensing type into the modern drop-ondemand (DOD) printers, with thermal or piezoelectric print heads.…”

Section: Introductionmentioning

confidence: 99%