Design and fabrication of biocompatible wrinkled hydrogel films with selective antibiofouling properties

González‐Henríquez, Carmen M.; Sarabia-Vallejos, Mauricio A.; Terraza, Claudio A.; Campo, Adolfo del; López‐Martínez, Elena; Cortajarena, Aitzibier L.; Casado-Losada, Isabel; Martínez-Campos, Enrique; Rodríguez‐Hernández, Juan

doi:10.1016/j.msec.2018.12.061

Cited by 21 publications

(24 citation statements)

References 31 publications

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“…In Figure 6 are plotted the tendency of both the amplitude and the wavelength of the wrinkled patterns formed as a function of the vacuum exposure times. These results were obtained from a specially designed image analysis code based on MatLab [ 8 , 37 ], which measures automatically the width and the height of the wrinkles from a multi-level Otsu segmented image. The width of each wrinkle is obtained from the image Hough transformation (ellipse adjustment) and the height is estimated from a Gaussian single-peak fitting of the AFM micrograph profiles ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

“…In this context, surface properties have been reported to be critical in the development of novel biomaterials. Accordingly, it has been possible to fabricate selective biofouling surfaces which can stimulate cell growth while avoiding bacterial colonization, just by changing the scale of the micro-patterns present on the surface [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among the methodologies based on surface instabilities, which include among others dewetting, breath figure formation, electrohydrodynamic patterning, thermal-gradient-induced surface structuration, and reaction–diffusion surface patterning, wrinkle formation is probably one of the most intensively explored approaches to produce micro- and nanometer size functionalized wrinkled surfaces due to their interesting applications for the elaboration of optical surfaces, as templates for microparticle arrays, the elaboration of flexible electronics, or the control of the adhesive properties of the surface [ 8 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Micrometric Wrinkled Patterns Spontaneously Formed on Hydrogel Thin Films via Argon Plasma Exposure

et al. 2019

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The generation of microstructured patterns on the surface of a specific polymeric material could radically improve their performance in a particular application. Most of the interactions with the environment occur at the material interface; therefore, increasing the exposed active surface considerably improves their range of application. In this article, a simple and reliable protocol to form spontaneous wrinkled patterns using a hydrogel layer is reported. For this purpose, we took advantage of the doctor blade technique in order to generate homogenous films over solid substrates with controlled thickness and large coverage. The hydrogel wrinkle formation involves a prepolymerization step which produces oligomers leading to a solution with increased viscosity, enough for doctor blade deposition. Subsequently, the material was exposed to vacuum and plasma to trigger wrinkled pattern formation. Finally, a UV-polymerization treatment was applied to fix the undulations on top. Interestingly, the experimental parameters allowed us to finely tune the wrinkle characteristics (period, amplitude, and orientation). For this study, two main aspects were explored. The first one is related to the role of the substrate functionalization on the wrinkle formation. The second study correlates the deswelling time and its relationship with the dimensions and distribution of the wrinkle pattern. In the first batch, four different 3-(trimethoxysilyl)propyl methacrylate (TSM) concentrations were used to functionalize the substrate in order to enhance the adhesion between hydrogel film and the substrate. The wrinkles formed were characterized in terms of wrinkle amplitude, wavelength, pattern roughness, and surface Young modulus, by using AFM in imaging and force spectroscopy modes. Moreover, the chemical composition of the hydrogel film cross-section and the effect of the plasma treatment were analyzed with confocal Raman spectroscopy. These results demonstrated that an oxidized layer was formed on top of the hydrogel films due to the exposure to an argon plasma.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micrometric Wrinkled Patterns Spontaneously Formed on Hydrogel Thin Films via Argon Plasma Exposure

et al. 2019

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“…The application of Fourier analysis for periodic feature size determination could be used to quantify how changes in material preparation parameters affect resulting properties including but not limited to elastic modulus, hydrophobicity or hydrophilicity, conductivity, adhesion, fouling, stretchability, and optical properties. Specifically, accurate characterization of periodic feature size could be applied to many areas of study including the extraction of mechanical moduli from buckled films; [15,16,22,23] assessment of how drying or crosslinking parameters affect the topography of wrinkled hydrogel systems; [43] analysis of cell growth, proliferation, and alignment as a function of surface topography; [44,45] quantification of the effect of structure size on anti-fouling properties; [26] and the characterization of current and resistance of stretchable electronics as a function of wrinkle size. [24]…”

Section: Fourier Analysis Of Thermally Structured Goldmentioning

confidence: 99%

“…[6,19,20] Recently developed surface structuring methods take advantage of surface instabilities that arise from strain dissipation in rigid materials. Surface wrinkling is an extensively studied example of structuring based on surface instabilities, and has been used to produce functional materials with controllable properties, such as mechanical properties, [15,16,22,23] wettability, [13,20] conductivity, [4,24] cell proliferation, [25] adhesion, [13] anti-fouling, [25,26] stretchability, [13,24] and optical properties. [15] These structuring techniques make it possible to engineer materials with tunable properties, which has aided in the development of smaller, more cost effective, and higher sensitivity materials.…”

Section: Introductionmentioning

confidence: 99%

Image Analysis of Structured Surfaces for Quantitative Topographical Characterization

Stimpson¹,

Wagner²,

Cranston³

et al. 2020

Preprint

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<p>In the fields of functional materials, interfacial chemistry, and microscale devices, surface structuring provides an opportunity to engineer materials with unique tunable properties such as wettability, anti-fouling, crack propagation, and specific surface area. Often, the resulting properties are related to the feature sizes of the structured surfaces and therefore, it is necessary to accurately quantify these topographies. This work presents a step-by-step description of a method for the quantification of the size of periodic structures using 2D discrete Fourier Transform analysis coupled with data filtering techniques to optimize feature size extraction and reduce user bias and error. The method is validated using artificial images of periodic patterns as well as scanning electron microscopy images of gold films that are structured on different substrates. While image Fourier Transform has been used previously and is a built-in feature in some commercial and open-source image analysis software, this work details image pre-processing and feature extraction steps, and how to best apply them, which has not been described in detail elsewhere. This method can analyze engineered or natural periodic topographies (e.g., wrinkles) to enable the design of patterned materials for applications including photovoltaics, biosensors, tissue engineering, flexible electronics, and thin film metrology.</p>

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Microstructuring of Thermoresponsive Biofunctional Hydrogels by Multiphoton Photocrosslinking

Morozov,

Wiesner (née Diehl),

Grün

et al. 2024

Adv Funct Materials

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A pioneering method is reported for creating thermoresponsive biofunctional hydrogel microstructures using maskless multiphoton lithography. Departing from conventional multiphoton‐triggered polymerization‐based techniques, this approach relies on simultaneous photocrosslinking and attachment of already pre‐synthesized polymer chains onto solid substrates. The method allows improving control over polymer network characteristics and enables facile integration of additional functionalities through postmodification with biomolecules at specific sites. Exploring two distinct benzophenone‐ and anthraquinone‐based photocrosslinkers incorporated into specially designed poly(N‐isopropyl acrylamide)‐based co‐ and terpolymers, the photocrosslinking efficacy is scrutinized with the use of a custom femtosecond near‐infrared laser lithographer. Comprehensive characterization via surface plasmon resonance imaging, atomic force microscopy, and optical fluorescence microscopy reveals swelling behavior and demonstrates postmodification feasibility. Notably, within a specific range of multiphoton photocrosslinking parameters, the surface‐attached microstructures exhibit a quasiperiodic topography akin to wrinkle‐pattern formation. Leveraging the capabilities of established multiphoton lithographer systems that offer fast pattern writing with high resolution, this approach holds great promise for the versatile fabrication of multifunctional 3D micro‐ and nanostructures. Such tailored responsive biofunctional materials with spatial control over composition, swelling behavior, and postmodification are particularly attractive in the areas of bioanalytical and biomedical technologies.

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Design and fabrication of biocompatible wrinkled hydrogel films with selective antibiofouling properties

Cited by 21 publications

References 31 publications

Micrometric Wrinkled Patterns Spontaneously Formed on Hydrogel Thin Films via Argon Plasma Exposure

Micrometric Wrinkled Patterns Spontaneously Formed on Hydrogel Thin Films via Argon Plasma Exposure

Image Analysis of Structured Surfaces for Quantitative Topographical Characterization

Microstructuring of Thermoresponsive Biofunctional Hydrogels by Multiphoton Photocrosslinking

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