Design and fabrication of novel multi-targeted magnetic nanoparticles for gene delivery to breast cancer cells

Amani, Amin; Alizadeh, Mohammad; Yaghoubi, Hashem; Ebrahimi, Hossein Ali

doi:10.1016/j.jddst.2020.102151

“…In other words, the hydrophilic polymers have been shown to accelerate the rate of DNA release by forming water channels within the matrix. Recent studies [32][33][34] have reported the ability of copolymers containing PLA and PEG components to control drug release, which is in support of the results of this study. The ability to penetrate and transport PLA-PEG/Chitosan-FA/DNA nanoparticles into MCF-7 cells was demonstrated using uorescent microscopy images (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…4, A, B, C and D). Given that the premise for gene transfer by these nanocarriers is dependent on the phenomena of endocytosis, and that intracellular nucleases destroy more than 99 percent of the DNA entering the cell, as a result, in gene therapy research, protecting DNA from harmful enzymes is critical [33]. Previous studies have demonstrated that polycations constitute a physical barrier to restriction enzyme binding due to their complexation with DNA, therefore shielding DNA from digestion by these enzymes [34].The ability of PLA-PEG/Chitosan-FA/DNA nanoparticles to penetrate and safely release DNA into MCF-7 cells was demonstrated by uorescent microscopy images of MCF-7 cells treated with these nanoparticles (Fig.…”

Section: Discussionmentioning

confidence: 99%

Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Afrouz

¹

,

Ahmadi-Nouraldinvand

²

,

ajirlu

³

et al. 2022

Preprint

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The ability of Chitosan-Folic acid and PLA-PEG polymers for gene delivery into MCF-7 cells was evaluated. The PLA-PEG copolymer is one of the most appealing nanoparticles for gene transfer to human cells because of its biocompatibility and high blood circulation. However, one of the barriers to the use of these polymers in gene therapy has always been the low effectiveness of these nanoparticles. In this study, Chitosan and folic acid were utilized to improve gene transfer efficiency and encapsulation of PLA-PEG/Chitosan-FA/DNA nanoparticles. PLA-PEG/Chitosan-FA/DNA nanoparticles with different concentrations of Chitosan-FA were prepared using double emulsion-solvent evaporation technique. The biological properties (i.e., biocompatibility, DNA release, and gene transfer ability to MCF-7 cells in vitro) as well as the physical and chemical properties (i.e., particle size, zeta potential, and the morphology of the resultant nanoparticles) of PLA-PEG/Chitosan-FA/DNA nanoparticles were investigated. The results showed that increasing the Chitosan-FA concentration in the PLA-PEG/Chitosan-FA/DNA nanoparticles resulted in an increase in zeta potential, DNA release, encapsulation, and gene delivery efficiency. The MTT assay showed PLA-PEG/Chitosan-FA/DNA nanoparticles exhibit low cytotoxicity and good compatibility. On the other hand, fluorescence microscopy and flow cytometry were used to test the ability of PLA-PEG/Chitosan-FA/DNA nanoparticles with varied concentrations of chitosan-folic acid to transfer the gene to MCF-7 cells. Flow cytometry and fluorescence microscopy analysis showed that increasing the concentration of chitosan-folic acid in PLA-PEG/Chitosan-FA/DNA nanoparticles improved gene transfer efficiency to MCF-7 cells.

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“…In other words, the hydrophilic polymers have been shown to accelerate the rate of DNA release by forming water channels within the matrix. Recent studies (Amani et al 2021;Guo et al 2021;Mateti et al 2021) have reported the ability of copolymers containing PLA and PEG components to control drug release, which is in support of the results of this study. The ability to penetrate and transport PLA-PEG/Chitosan-FA/DNA nanoparticles into MCF-7 cells was demonstrated using uorescent microscopy images (Fig.…”

Section: Discussionsupporting

confidence: 90%

Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Afrouz

¹

,

Ahmadi-Nouraldinvand

²

,

Ajirlu

³

et al. 2022

Preprint

Self Cite

0

View full text Add to dashboard Cite

The ability of Chitosan-Folic acid and PLA-PEG polymers for gene delivery into MCF-7 cells was evaluated. The PLA-PEG copolymer is one of the most appealing nanoparticles for gene transfer to human cells because of its biocompatibility and high blood circulation. However, one of the barriers to the use of these polymers in gene therapy has always been the low effectiveness of these nanoparticles. In this study, Chitosan and folic acid were utilized to improve gene transfer efficiency and encapsulation of PLA-PEG/Chitosan-FA/DNA nanoparticles. PLA-PEG/Chitosan-FA/DNA nanoparticles with different concentrations of Chitosan-FA were prepared using double emulsion-solvent evaporation technique. The biological properties (i.e., biocompatibility, DNA release, and gene transfer ability to MCF-7 cells in vitro) as well as the physical and chemical properties (i.e., particle size, zeta potential, and the morphology of the resultant nanoparticles) of PLA-PEG/Chitosan-FA/DNA nanoparticles were investigated. The results showed that increasing the Chitosan-FA concentration in the PLA-PEG/Chitosan-FA/DNA nanoparticles resulted in an increase in zeta potential, DNA release, encapsulation, and gene delivery efficiency. The MTT assay showed PLA-PEG/Chitosan-FA/DNA nanoparticles exhibit low cytotoxicity and good compatibility. On the other hand, fluorescence microscopy and flow cytometry were used to test the ability of PLA-PEG/Chitosan-FA/DNA nanoparticles with varied concentrations of chitosan-folic acid to transfer the gene to MCF-7 cells. Flow cytometry and fluorescence microscopy analysis showed that increasing the concentration of chitosan-folic acid in PLA-PEG/Chitosan-FA/DNA nanoparticles improved gene transfer efficiency to MCF-7 cells.

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“…However, with continuous development in material technology, magnetic core resources have diversified [66]. Many high-performance silica-MNPs [65][66][67][68][69] have been reported and recent advances and product characteristics are shown in used in selective extraction, detection, and gene delivery [20,21,23,70].…”

Section: Preparation Of Silica-coated Mnpsmentioning

confidence: 99%

Solid‐phase extraction methods for nucleic acid separation. A review

Li

¹

,

Li

²

,

Yue

³

et al. 2021

J of Separation Science

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The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.

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Design and fabrication of novel multi-targeted magnetic nanoparticles for gene delivery to breast cancer cells

Cited by 19 publications

References 57 publications

Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

Solid‐phase extraction methods for nucleic acid separation. A review

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