Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Prada, Jorge; Cordes, Christina; Harms, Carsten; Lang, Walter

doi:10.3390/s19051178

Cited by 18 publications

(9 citation statements)

References 49 publications

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“…Prada et al. [ 205 ] introduced a disposable microfluidics‐based biosensor to monitor bacteria pollution. The biosensor was based solely on the COC from TOPAS grade 5013 and was designed to perform heating, cooling, and fluorosence‐based detection on a single chip.…”

Section: Applications Of Cocmentioning

confidence: 99%

A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

Agha

Waheed

Alamoodi

et al. 2022

Macro Materials & Eng

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Cyclic olefin copolymers (COC) are amorphous, transparent thermoplastics composed of cyclic olefin monomers (norbornene) and linear olefins (ethene). They are increasingly utilized as fabrication materials for microsystems and microfluidic devices, owing to their promising features of low water absorption, high electrical insulation, long-term stability of surface treatments, and resistance to a broad variety of acids and solvents. Many manufacturing processes for COC-based devices have been developed in recent decades. These methodologies are categorized as replication methods or fast prototyping as common in fabrication of thermoplastic microfluidic devices. This review gives a full discussion of the features of COCs, the various production processes, and the numerous selected applications in microfluidic platforms. The review also explores COC's composition and fundamental features, as well as fabrication processes and applications in a variety of fields, investigates the material's potential advantages and uses, and attempts to create a comprehensive list of COC's possible benefits. Due to their unique features and simplicity of fabrication, COCs are projected to advance the future of microfluidics, microsystems, and optofluidics.

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Section: Applications Of Cocmentioning

confidence: 99%

A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

Agha

Waheed

Alamoodi

et al. 2022

Macro Materials & Eng

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“…Considering these requirements, the cyclic olefin copolymer (COC) was proposed as a suitable material [37,38]. DNA oligonucleotide probes are easily immobilized on the COC surface through UV irradiation without surface modification [39], making it well suited for the fabrication of a chipbased biosensor [40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

Kim

Lim

et al. 2022

Biotechnol Bioproc E

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This study aimed to develop a label-free fluorescent aptasensor for the detection of diazinon (DZN) on a cyclic olefin copolymer (COC) substrate. The aptasensor design was based on rolling circle amplification (RCA) technology and the use of self-assembled copper nanoparticles (CuNPs). A dual-function (DF) probe, capable of binding to circular DNA and an aptamer, was designed and immobilized on a COC-bottom 96-well plate. An aptamer was used for selective recognition of DZN, and the specific site of the aptamer that strongly reacted with DZN was successfully identified using circular dichroism (CD) analysis. In presence of DZN, the aptamer and DZN formed a strong complex, thus providing an opportunity for hybridization of the DF probe and circular DNA, thereby initiating an RCA reaction. Repetitive poly thymine (T) sequence with a length of 30-mer, generated in the RCA reaction, served as a template for the synthesis of fluorescent copper nanoparticles, emitting an orange fluorescence signal (at approximately 620 nm) proportional to the amount of RCA product, within 10 min under UV irradiation. The CuNP fluorescence was imaged and quantified using an image analysis software. A linear correlation of the fluorescence signal was confirmed in the DZN concentration range of 0.1-3 ppm, with a detection limit of 0.15 ppm. Adoption of a label-free detection method, utilizing RCA and fluorescent CuNPs on COC substrates, reduced the need for complex equipment and requirements for DZN analysis, thereby representing a simple and rapid sensing method circumventing the limitations of current complex and labor-intensive methods.

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“…Cyclic olefin copolymer (COC) is an amorphous thermoplastic composed of ethylene and norbornene polymers at varying ratios, utilized for small electronics due to its performance characteristics such as optical transparency, thermostability, and chemical resistance [13][14][15]. In the last few years, there has been an increase interest in the utilization of COC as biosensor substrate, specifically in microfluidics due to its micropatterning ease and biocompatibility [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

et al. 2021

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A fast PCR-assisted impedimetric biosensor was developed for the selective detection of the clbN gene from the polyketide synthase (pks) genomic island in real Escherichia coli samples. This genomic island is responsible for the production of colibactin, a harmful genotoxin that has been associated with colorectal cancer. The experimental protocol consisted of immobilizing the designated forward primer onto an Au electrode surface to create the sensing probe, followed by PCR temperature cycling in blank, positive, and negative DNA controls. Target DNA identification was possible by monitoring changes in the system's charge transfer resistance values (R ct ) before and after PCR treatment through electrochemical impedance spectroscopy (EIS) analysis. Custom-made, flexible gold electrodes were fabricated using chemical etching optical lithography. A PCR cycle study determined the optimum conditions to be at 6 cycles providing fast results while maintaining a good sensitivity. EIS data for the DNA recognition process demonstrated the successful distinction between target interaction resulting in an increase in resistance to charge transfer (R ct ) percentage change of 176% for the positive DNA control vs. 21% and 20% for the negative and non-DNA-containing controls, respectively. Results showed effective fabrication of a fast, PCR-based electrochemical biosensor for the detection of pks genomic island with a calculated limit of detection of 17 ng/μL.

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Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Cited by 18 publications

References 49 publications

A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

A Review of Cyclic Olefin Copolymer Applications in Microfluidics and Microdevices

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

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