Design and operation of a microfluidic sorter for Drosophila embryos

Chen, C.C.; Zappe, S.; Şahin, Özgür; Zhang, X.J.; Fish, Matt; Scott, Matthew P.; Solgaard, Olav

doi:10.1016/j.snb.2003.10.015

Cited by 41 publications

(39 citation statements)

References 5 publications

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“…12 Furthermore, high throughput screening of microdroplets at frequencies in excess of 1 kHz is possible using such systems. [18][19][20][21] These properties suggest that microfluidic technology could be a valuable tool for the study of microalgae.…”

Section: Insight Innovation Integrationmentioning

confidence: 99%

Quantitative tracking of the growth of individual algal cells in microdroplet compartments

et al. 2011

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In this paper we introduce a simple droplet-based microfluidic system consisting of two separate devices to encapsulate and culture microalgae, in contrast to cultivation in bulk liquid medium. This microdroplet technology has been used to monitor the growth of individual microalgal cells in a constant environment for extended periods of time. Single cells from three species of green microalgae, (two freshwater species Chlamydomonas reinhardtii and Chlorella vulgaris, and one saline species Dunaliella tertiolecta), were encapsulated and incubated in microdroplet compartments of diameter of B80 mm, and their growth analysed over 10 days. In all cases, the doubling time of microalgae grown in microdroplets was similar to growth in bulk. The growth of C. reinhardtii in microdroplets of varying diameters and with different initial cell numbers per droplet was investigated, as well as the effect of varying medium conditions such as pH and nitrogen concentration. This methodology offers the opportunity to study characteristics over time of individual cells and colonies, as well as to screen large numbers of them.

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Section: Insight Innovation Integrationmentioning

confidence: 99%

Quantitative tracking of the growth of individual algal cells in microdroplet compartments

et al. 2011

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“…Additionally, the introduction of integrated microfluidics facilitates more complex biological manipulations in one single device [22]. All these advancements have allowed the miniaturization of filtration devices and the realization of precise channel geometries, leading to the more sophisticated separation of cells in microfluidics, such as the isolation of red blood cells (RBCs) and leukocytes [24][25][26], embryos [27], and cancer cells [28,29].…”

Section: Introductionmentioning

confidence: 99%

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Liu

Wang

et al. 2010

Electrophoresis

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Metastasis caused by the entry of circulating tumor cells (CTCs) into the bloodstream or lymphatic vessels is a major factor contributing to death in cancer patients. Separation of CTCs and studies on CTC-drug interactions are very important for prognostic and therapeutic implications of metastatic cancer. In this study, an integrated microfluidic device for CTC separation through the combination of lectin and microstructure is presented. This microfluidic device and lectin concanavalin A were utilized for the separation of K562 cells in whole blood samples. The results showed that the separation efficiency can reach 84%, which is much higher than that of an experiment without concanavalin A treatment. To further demonstrate the feasibility of this microfluidic device application in sequential studies after target cells were separated, the interactions of K562 cells and an anticancer drug, cytarabine, were also examined. After 6 h on-chip treatment with cytarabine, the viabilities of K562 cells were 85.29, 77.05, and 40% for drug concentration levels of 0.25, 0.5, and 1.0 g/L, respectively. This system can facilitate the rapid and efficient in vitro investigation of CTC separation and CTC-related studies.

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“…Recently, microfluidic devices and Micro-Electrical-Mechanical Systems (MEMS) have been successfully used in many Drosophila assays, [38][39][40][41][42][43][44][45][46] mainly because of their capability to provide scalable and controllable environments for quantitative embryonic [38][39][40][41][42][43] and larval [44][45][46] investigations in flies. These miniaturized devices that are made primarily from the elastomer polydimethylsiloxane (PDMS) have significantly enhanced the throughput, accuracy, and reproducibility of these assays.…”

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confidence: 99%

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

et al. 2015

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Drosophila melanogaster (fruit fly) is a model organism and its behaviours including oviposition (egg-laying) on agar substrates have been widely used for assessment of a variety of biological processes in flies. Physical and chemical properties of the substrate are the dominant factors affecting Drosophila's oviposition, but they have not been investigated precisely and parametrically with the existing manual approaches. As a result, many behavioral questions about Drosophila oviposition, such as the combined effects of the aforementioned substrate properties (e.g., exposure area, sugar content, and stiffness) on oviposition and viability, and their threshold values, are yet to be answered. In this paper, we have devised a simple, easily implementable, and novel methodology that allows for modification of physical and chemical composition of agar substrates in order to quantitatively study survival and oviposition of adult fruit flies in an accurate and repeatable manner. Agar substrates have been modified by surface patterning using single and hexagonally arrayed through-hole polydimethylsiloxane (PDMS) membranes with various diameters and interspacing, as well as by substrate stiffness and sugar content modification via alteration of chemical components. While pure PDMS substrates showed a significant lethal effect on flies, a 0.5 mm diameter through-hole access to agar was found to abruptly increase the survival of adult flies to more than 93%. Flies avoided ovipositing on pure PDMS and on top of substrates with 0.5 mm diameter agar exposure areas. At a hole diameter of 2 mm (i.e., 0.25% exposure area) or larger, eggs were observed to be laid predominately inside the through-holes and along the edges of the PDMS-agar interface, showing a trending increase in site selection with 4 mm (i.e., 1% exposure area threshold) demonstrating natural oviposition rates similar to pure agar. The surfacemodified agar-PDMS hybrid devices and the threshold values reported for the substrate physical and chemical conditions affecting oviposition are novel; therefore, we advocate their use for future in-depth studies of oviposition behaviour in Drosophila melanogaster with accuracy and repeatability. The technique is also useful for development of novel assays for learning and decision-making studies as well as miniaturized devices for self-assembly of eggs and embryonic developmental investigations. V C 2015 AIP Publishing LLC. [http://dx

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Design and operation of a microfluidic sorter for Drosophila embryos

Cited by 41 publications

References 5 publications

Quantitative tracking of the growth of individual algal cells in microdroplet compartments

Quantitative tracking of the growth of individual algal cells in microdroplet compartments

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

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