A transportation system requires an asymmetry to achieve objects’ motion on an oscillating surface. Transportation methods based on vibrational techniques usually employ different types of asymmetries, such as temporal (time) asymmetry, kinematic asymmetry, wave asymmetry or power asymmetry. However, transporting an object on an inclined angle requires a relatively high net frictional force over each period of vibrational cycles due to the gravitational potential energy exerted on the object. This paper investigates the transportation of an object upward on an inclined plane that harmonically oscillates in its longitudinal direction. The novelty of this research is attributed to the upward motion of the object on the inclined plane, which is achieved by creating an additional asymmetry of the system through dry friction dynamic manipulations. For this reason, periodic dynamic dry friction manipulations have been employed to create the asymmetry of frictional conditions, resulting in a net frictional force that outweighs the gravitational force. A mathematical model has been developed using the Lagrange method, which describes the moving object’s motion. Moreover, the theoretical findings and results confirmed that the object’s velocity and direction can be controlled by dynamic dry friction manipulations. To demonstrate the technical feasibility of the proposed method, an experimental investigation was carried out where the results demonstrated that the control parameters significantly influence the characteristics of the directional motion of the moving object. This transportation method is beneficial for various modern industries engaged in transportation and manipulation tasks with objects spanning a broad range of sizes, including those operating at small scales for applications in lab-on-a-chip technology, micro-assembly lines, micro-feeder systems and other delicate component manipulation systems. The presented research advances the classical theories of vibrational transportation on inclined surfaces.