Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

Soliman, Sara M.; Mosallam, Shaimaa; Mamdouh, Mohamed A; Hussein, Mohammed A.; El-Halim, Shady M. Abd

doi:10.1080/10717544.2022.2032875

Cited by 27 publications

(10 citation statements)

References 61 publications

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“…Measurements of PS, PDI, and ZP of the prepared formulations were achieved by using Malvern Zetasizer (Malvern Instruments Ltd., UK) via dynamic light scattering technique at 25 °C. The measurements were attained after appropriate dilution [ 35 , 36 ]. All assessments (EE%, PS, PDI, and ZP) were performed in triplicate ± SD.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Synergistic Effect of Bergamot Essential Oil with Spironolactone Loaded Nano-Phytosomes for Treatment of Acne Vulgaris: In Vitro Optimization, In Silico Studies, and Clinical Evaluation

et al. 2023

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The foremost target of the current work was to formulate and optimize a novel bergamot essential oil (BEO) loaded nano-phytosomes (NPs) and then combine it with spironolactone (SP) in order to clinically compare the efficiency of both formulations against acne vulgaris. The BEO-loaded NPs formulations were fabricated by the thin-film hydration and optimized by 32 factorial design. NPs’ assessments were conducted by measuring entrapment efficiency percent (EE%), particle size (PS), polydispersity index (PDI), and zeta potential (ZP). In addition, the selected BEO-NPs formulation was further combined with SP and then examined for morphology employing transmission electron microscopy and three months storage stability. Both BEO-loaded NPs selected formula and its combination with SP (BEO-NPs-SP) were investigated clinically for their effect against acne vulgaris after an appropriate in silico study. The optimum BEO-NPs-SP showed PS of 300.40 ± 22.56 nm, PDI of 0.571 ± 0.16, EE% of 87.89 ± 4.14%, and an acceptable ZP value of −29.7 ± 1.54 mV. Molecular modeling simulations showed the beneficial role of BEO constituents as supportive/connecting platforms for favored anchoring of SP on the Phosphatidylcholine (PC) interface. Clinical studies revealed significant improvement in the therapeutic response of BEO-loaded NPs that were combined with SP over BEO-NPs alone. In conclusion, the results proved the ability to utilize NPs as a successful nanovesicle for topical BEO delivery as well as the superior synergistic effect when combined with SP in combating acne vulgaris.

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Section: Methodsmentioning

confidence: 99%

Exploring the Synergistic Effect of Bergamot Essential Oil with Spironolactone Loaded Nano-Phytosomes for Treatment of Acne Vulgaris: In Vitro Optimization, In Silico Studies, and Clinical Evaluation

et al. 2023

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“…The determination of morphology of the optimized formula was throughout utilizing TEM (JEM-1230, Joel, Japan) (Al-mahallawi et al, 2021 ). Samples were installed on a grid surface of carbon-coated and negatively stained with one percent of the aqueous solution of phosphotungstic acid then dried at room temperature prior visualization (M. Soliman et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Fabrication of nanostructured lipid carriers ocugel for enhancing Loratadine used in treatment of COVID-19 related symptoms: statistical optimization, in-vitro, ex-vivo, and in-vivo studies evaluation

et al. 2022

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Loratadine (LORA), is a topical antihistamine utilized in the treatment of ocular symptoms of COVID-19. The study aimed to develop a Loratadine Nanostructured Lipid Carriers Ocugel (LORA-NLCs Ocugel), enhance its solubility, trans-corneal penetrability, and bioavailability. full-factorial design was established with 2 4 trials to investigate the impact of several variables upon NLCs properties. LORA-NLCs were fabricated by using hot melt emulsification combined with high-speed stirring and ultrasonication methods. All obtained formulae were assessed in terms of percent of entrapment efficiency (EE%), size of the particle (PS), zeta potential (ZP), as well as in-vitro release. Via using Design Expert® software the optimum formula was selected, characterized using FTIR, Raman spectroscopy, and stability studies. Gel-based of optimized LORA-NLCs was prepared using 4% HPMC k100m which was further evaluated in terms of physicochemical properties, Ex-vivo, and In-vivo studies. The optimized LORA-NLCs, comprising Compritol 888 ATO ® , Labrasol ® , and Span ® 60 showed EE% of 95.78 ± 0.67%, PS of 156.11 ± 0.54 nm, ZP of −40.10 ± 0.55 Mv, and Qh6% of 99.67 ± 1.09%, respectively. Additionally, it illustrated a spherical morphology and compatibility of LORA with other excipients. Consequently, gel-based on optimized LORA-NLCs showed pH (7.11 ± 0.52), drug content (98.62%± 1.31%), viscosity 2736 cp, and Q12% (90.49 ± 1.32%). LORA-NLCs and LORA-NLCs Ocugel exhibited higher ex-vivo trans-corneal penetrability compared with the aqueous drug dispersion. Confocal laser scanning showed valuable penetration of fluoro-labeled optimized formula and LORA-NLCs Ocugel through corneal. The optimized formula was subjected to an ocular irritation test (Draize Test) that showed the absence of any signs of inflammation in rabbits, and histological analysis showed no effect or damage to rabbit eyeballs. C max and the AUC 0–24 were higher in LORA-NLCs Ocugel compared with pure Lora dispersion-loaded gel The research findings confirmed that NLCs could enhance solubility, trans-corneal penetrability, and the bioavailability of LORA.

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“…One of the best source of flavanols is cranberry fruits (Manach et al, 2004;Viskelis et al, 2009). The most common flavanol in cranberries is quercetin, as 3-o-galactoside, anthocyanin, myricetin and kaempferol (Soliman et al, 2022).…”

Section: Advances Inmentioning

confidence: 99%

Cranberry Extract; as a Promising Functional Food to Regulate SREBP1/PPAR–α/CPT-1/ACO Signaling Pathways in HFD-Induced Obesity in Rats

Fayed¹,

Ibrahem²,

Hassan³

et al. 2022

AAVS

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The correlation between obesity and metabolic disorders like Type 2 diabetes, cardiovascular disease, and several types of cancer has made obesity a challenge for world health. The current study's objective was to assess the antioxidant capacity of cranberry extract in high-fat diet-induced obesity. For eight weeks, 36 white albino rats weighing 197±10 g were selected at random to five groups: Group I was administered a regular diet; Group II was fed a high-fat diet; Group III was given a high-fat diet plus cranberry extract (75 mg/kg); Group IV was fed a highfat diet with cranberry extract (150 mg/kg); and Group V was fed a high-fat diet plus metformin (500 mg/kg). The lipid profile was determined using blood samples, and liver tissue samples were used to determine glucose, insulin, leptin, TC, TG, HDL-C, ALT, AST, MDA, TNF-α, PCO. The HFD administrated rats resulted in increased total body weight, glucose, insulin, leptin, cholesterol, triglycerides, MDA, ALT, AST, TNFα and PCO levels, as well as SREBP1 gene expression, according to the findings. However, our findings revealed a significant reduction in HDL-C, GSH, CAT and PON 1 enzyme levels, the expression of the PPARα, CPT I and ACO gene in HFD treated rats. Also, administration of cranberry extract and metformin significantly normalized body weight, blood glucose, insulin, leptin, total cholesterol, triglycerides, HDL-C, TBARS, GSH, CAT, ALT, AST, TNF-and PCO and PON 1 as well as SREBP1, PPARα, CPT I and ACO levels. The biochemical and PCR results are supported by electrophoretic patterns and histological evidence. Conclusions: The findings imply that cranberry extract could be used as a new pharmaceutical drug in the treatment of obesity.

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Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats

Cited by 27 publications

References 61 publications

Exploring the Synergistic Effect of Bergamot Essential Oil with Spironolactone Loaded Nano-Phytosomes for Treatment of Acne Vulgaris: In Vitro Optimization, In Silico Studies, and Clinical Evaluation

Exploring the Synergistic Effect of Bergamot Essential Oil with Spironolactone Loaded Nano-Phytosomes for Treatment of Acne Vulgaris: In Vitro Optimization, In Silico Studies, and Clinical Evaluation

Fabrication of nanostructured lipid carriers ocugel for enhancing Loratadine used in treatment of COVID-19 related symptoms: statistical optimization, in-vitro, ex-vivo, and in-vivo studies evaluation

Cranberry Extract; as a Promising Functional Food to Regulate SREBP1/PPAR–α/CPT-1/ACO Signaling Pathways in HFD-Induced Obesity in Rats

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