Two cylinders arranged symmetrically on a frame have become a major form of steering mechanism for articulated off-road vehicles (AORVs). However, the differences of stroke and arm lead to pressure fluctuation, vibration noise, and a waste of torque. In this paper, the differences of stroke and arm are reduced based on a genetic algorithm (GA). First, the mathematical model of the steering mechanism is put forward. Then, the difference of stroke and arm are optimized using a GA. Finally, a FW50GLwheel loader is used as an example to demonstrate the proposed GA-based optimization method, and its effectiveness is verified by means of automatic dynamic analysis of mechanical systems (ADAMS). The stroke difference of the steering hydraulic cylinders was reduced by 92% and the arm difference reached a decrease of 78% through GA optimization, in comparison with unoptimized structures. The simulation result shows that the steering mechanism optimized by GA behaved better than by previous methods.