In this paper a Dual Band Microstrip Patch Antenna with U-shaped Defected Ground Structure at 5.9 GHz and 27 GHz is designed and analyzed to cater wireless applications. The wideband behavior of the antenna is achieved by inserting U-shape DGS structure in the ground plane. The parameters such as gain, reflection coefficient (S11), Voltage Standing Wave Ratio (VSWR), radiation pattern, and percentage bandwidth is evaluated to measure antenna performance. Here two frequencies are considered for wireless applications. One is at 5.9 GHz for Wi-Fi as well as Wi-Max applications. The second is at 24.4 GHz and 27 GHz for 5G network applications. The High-Frequency Structure Simulator tool is used to simulate the proposed antenna. Here, RT 5880 Duroid material of thickness 1.6 mm with a relative dielectric constant of 2.2 and substrate size of 36 mm x 40 mm with line feeding technique is considered for experimentation. From the simulation results, it is observed that the antenna achieves a bandwidth of 170 MHz with a gain of 14 dB, S11 of -15.52 dB, and VSWR of 1.4 at a resonant frequency of 5.9 GHz. Further, the bandwidth of 840 MHz with a gain of 15.75 dB, S11 of -18.37 dB, and VSWR of 1.35 is achieved at the resonant frequency of 24.4 GHz. Whereas, wide bandwidth with a gain of 3.28 dB, S11 of -14.79 dB, and VSWR of 1.45 at a resonant frequency of 27 GHz is achieved. The antenna can operate for 5.9 GHz, 24.4 GHz, and 27 GHz covering Wi-MAX and Mobile 5G applications. This work significantly improves the MSPA performance parameters as compared to existing techniques. Future scope lies in improving the impedance matching and reducing the surface currents.