Design and Performance of an Infrared Microscope Attachment*

Coates, Vincent; Offner, Abe; Siegler, Elijah

doi:10.1364/josa.43.000984

Cited by 77 publications

(15 citation statements)

References 17 publications

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“…This can be detected by examining the anisotropy of the infrared absorptions of an oriented film, which can be obtained by a unidirectional stroking of the wet film with a spatula. Since highly oriented regions may occur only over small areas of the film [47], the use of an infrared microscope [62,63] is advantageous because it allows examination of a small area (200 /x x 500 /x, for example) of the sample film. By the use of plane polarized radiation, infrared absorption intensities can be compared for two cases, when the electric vector of the incident radiation is respectively parallel and perpendicular to the direction of orientation.…”

Section: Experimental Methodsmentioning

confidence: 99%

Infrared and Raman Spectroscopy

Tsuboi¹

1974

Basic Principles in Nucleic Acid Chemistry

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Section: Experimental Methodsmentioning

confidence: 99%

Infrared and Raman Spectroscopy

Tsuboi¹

1974

Basic Principles in Nucleic Acid Chemistry

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“…Coates et al (10) studied IR microscopes that could be equipped with IR spectrometers. However, because of low available energy of thermal sources and single-element detector, the IMS instrument required precise optical alignment and the scan rate was very low and it took a long time for data collection.…”

Section: Stage I: Thermal Source and Single Element Detectormentioning

confidence: 99%

The Development of Infrared Microspectroscopy (IMS) and Its Applications in Agricultural and Aquatic Products

Zhou

Fang

2013

Applied Spectroscopy Reviews

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Infrared microspectroscopy (IMS) has undergone rapid development in the last 20 years. New infrared sources and detectors have promoted this development. More meaningful information can be achieved in less time, and spatial resolution is only several micrometers. The improved IMS is important in biology, medical science, and biochemistry. This review describes the progress of IMS in light sources and detectors, including thermal sources, synchrotron infrared sources, multiple synchrotron beams, single element detectors, as well as focal plane array (FPA) detectors. In addition, applications of IMS on agricultural and aquatic products such as cereal seeds, milk, fish, meat, forage grass, juice, and cheese are explored. Finally, new trends in the development of IMS and its importance in the fundamental and applied research of agricultural and aquatic products are discussed.

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“…A commercial IR microscope system described in 1953 by Coates et al [2] demonstrated quite respectable IR spectra from single fiber samples of less than 20 mm in diameter, recorded with 15 min scan times, and contained some design attributes that are still present in today's systems. However, it was not until early 1980s when the rapid uptake of commercial FT-IR systems and applications such as semiconductor microanalysis provided both the applications and technology interest to spur the growth in IR microspectroscopy.…”

Section: Developments In Ir Microscopy and Imaging Systemsmentioning

confidence: 99%

FT‐IR Imaging Hardware

Sellors

Hoult

Crocombe

et al. 2010

Raman, Infrared, and Near‐Infrared Chemical Imaging

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The combination of IR spectroscopy with visible microscopy has been used in a wide range of analytical applications for more than 20 years. More recently, however, IR microspectroscopy has benefited from developments in IR detector arrays leading to a marked growth in FT-IR imaging technologies and applications. It is now a fairly simple task to obtain a high-quality IR spectrum from a sample region of around 20 mm in matter of seconds, and the ability to collect full IR images containing hundreds of thousands of pixels, where every image pixel contains a full range IR spectrum, is now available in many hundreds of laboratories worldwide. IR imaging hardware is not yet mature, but despite this, with today's state-of-the-art FT-IR imaging systems, the analysis time for many applications is limited not by the speed at which quality images are obtained, but by the data analysis or sample preparation techniques at the disposal of the operator.Progress in commercial FT-IR imaging hardware development comes from various drivers, but two are particularly relevant: (a) the high popularity of single point IR microscopy systems that has fuelled the interest in technology and applications utilizing more rapid methods of data acquisition and (b) the development of multichannel array detectors that operate in the mid-infrared region for nonspectroscopic applications. These are fundamental to both the understanding of current FT-IR imaging technologies and probable developments in the near future. Developments in IR Microscopy and Imaging SystemsInterest in obtaining IR spectra from small samples goes back to over 60 years; for example, a reported study of the structure of penicillin by Thomson [1] in the late 1940s used a prismbased dispersive spectrometer coupled with a beam condenser/microscope system. A commercial IR microscope system described in 1953 by Coates et al. [2] demonstrated quite respectable IR spectra from single fiber samples of less than 20 mm in diameter, recorded with 15 min scan times, and contained some design attributes that are still present in today's systems. However, it was not until early 1980s when the rapid uptake of commercial FT-IR systems and applications such as semiconductor microanalysis provided both the applications and technology interest to spur the growth in IR microspectroscopy. Today, all major manufacturers of laboratory FT-IR spectrometers include IR microscopes, some with automated point mapping systems, as well as imaging systems in their product portfolios.Early (1980s) FT-IR microscope systems were mostly bolt-on accessories derived from optical microscope frames that were modified to support the collection of IR spectra from selected regions of interest (ROI). Later, systems were Raman, Infrared, and Near-Infrared Chemical Imaging Edited by Slobodan Š ašić and Yukihiro Ozaki

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Design and Performance of an Infrared Microscope Attachment*

Cited by 77 publications

References 17 publications

Infrared and Raman Spectroscopy

Infrared and Raman Spectroscopy

The Development of Infrared Microspectroscopy (IMS) and Its Applications in Agricultural and Aquatic Products

FT‐IR Imaging Hardware

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