Design and synthesis of amphiphilic core–shell nanoparticles with temperature sensitive-shell for controllable intracellular uptake

Trypsin treatment employed in traditional cell desorption method cause the damage of extracellular matrix protein and cell membrane integrity. Two‐dimensional thermo‐sensitive cell culture dish has the limitation of insufficient area. This report describes an ingenious design and synthesis of a thermoresponsive chitosan‐based hydrogel, N‐succinyl chitosan (NSCS) grafted poly(N‐isopropylacrylamide) (PNIPAM), named NSCS‐g‐PNIPAM. We then demonstrate the ultrafast gelation and liquefying of NSCS‐g‐PNIPAM by reversibly tuning the temperature of hydrogel solution above (gelation) or below (liquefying) 32°C. These unique features render the NSCS‐g‐PNIPAM hydrogel an ideal matrix for enzyme‐free three‐dimensional cell culture. As an exemplar, the skeleton muscle cell is chosen for in vitro cellular studies. The data suggests that after seven passages and harvesting, the amount of total protein, laminin and fibronectin of cell collected from the thermoreponsive hydrogel group shows unsignificant decrease after seven passages. The proteins on the cell surface are well protected compared to the conventional trypsin‐treated method. In addition, by labeling the NSCS‐g‐PNIPAM hydrogels with tetraphenylethylene (TPE)‐based aggregation‐induced‐emission (AIE) luminogenes, NSCS‐g‐PNIPAM were observed by confocal microscopy and they remain outside during entire cell culturing period.

show abstract

A ready‐to‐use fast gelation/liquefying hydrogel towards enzyme free three‐dimensional cell culture

Dong

Jiang

2022

Journal of Polymer Science

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Design and synthesis of amphiphilic core–shell nanoparticles with temperature sensitive-shell for controllable intracellular uptake

Cited by 1 publication

References 1 publication

A ready‐to‐use fast gelation/liquefying hydrogel towards enzyme free three‐dimensional cell culture

A ready‐to‐use fast gelation/liquefying hydrogel towards enzyme free three‐dimensional cell culture

Contact Info

Product

Resources

About