The 180 nm ground rule production prototype x-ray lithography aligner was developed for the Defense Advanced Lithography Program (DALP) and installed in IBM’s Advanced Lithography Facility (ALF) in 1995. This aligner is designed to satisfy the manufacturing requirement for 250 and 180 nm ground rule electronic devices, such as 256 Mbit and 1 Gbit dynamic random access memories. The acceptance evaluation of this aligner was presented elsewhere (Ref. 12). The aligner uses an innovative x-ray image sensor (XRIS) to align the mask by detecting its x-ray actinic image and uses an off-axis alignment system, similar to the alignment system used in Micrascan-II™ (a trademark of Silicon Valley Group Lithography), to align the wafer. From subsystem testing, the alignment repeatability of XRIS is not a significant contributor to the aligner’s contribution of overlay error. As a result, the x-ray alignment sensor technology can be used for future generations of x-ray lithography aligners. This article will specifically focus on the overlay performance of the aligner. The overlay evaluation consisted of careful wafer stage and alignment system calibration, off-line subsystem testing, and extensive exposure tests. The exposure tests were designed to measure the alignment performance of the aligner across a wide range of wafer structure types. A detailed discussion is given on the test methodology and the aligner contribution to the total overlay error. On the better levels (e.g., etched Si), the tool-to-itself aligner contribution to overlay error is in the range of 35–40 nm (mean+3σ) and approaches the overlay error budget for a 180 nm ground rule generation x-ray aligner. An overlay model was also developed to include the error contributions of the aligner as well as the image placement accuracy of the mask. The modeling results show that the aligner, with image placement errors of current x-ray masks, can satisfy the overlay requirement (mean+3σ<70 nm) for 1 Gbit device demonstration programs.