The rapid impact testing of bridges contains unique advantages. For example, structural parameters, including frequency response function and structural flexibility matrix can be identified; however, additional impact-testing instruments are required to excite a bridge, restricting the efficiency of the measurement strategy in terms of experimental cost and time. In this paper, a particle image velocimetry-based method is proposed for the rapid impact testing and system identification of footbridges under pedestrian excitations. The proposed method has shown promising features: (1) pedestrian load is utilized for the impact excitation of footbridges, which is more convenient than the conventional impacttesting method with additional excitation devices; (2) the human-induced impact forces under varying jumping scenarios are calculated from image sequences of human motions acquired by a single camera with its noncontact and target-less characteristics; and (3) both humaninduced impact forces (inputs) and structural responses (outputs) are employed to identify more modal parameters (i.e., scaling factors, modal mass, and structural flexibility). The robustness of the proposed method was successfully validated by a laboratory test of a simply supported beam and field testing of a cable-stayed footbridge. The proposed method not only could improve