Design and validation of a biomechanical bioreactor for cartilage tissue culture

Correia, V.; Panadero, J. A.; Ribeiro, Clarisse; Sencadas, Vítor; Rocha, Jorge; Ribelles, J. L. Gomez; Lanceros‐Méndez, S.

doi:10.1007/s10237-015-0698-5

Cited by 13 publications

(13 citation statements)

References 25 publications

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“…Together, all these properties make it a promising candidate material for biomedical applications, such as the fabrication of cardiac stents [30], wound dressing [31], drug release [32] and antitumor applications [33]. More specifically in tissue engineering, PHBV is usually employed for scaffolds in bone tissue regeneration [34,35], absorbable surgical sutures [36], among others.Additionally, owing to its piezoelectric properties (piezoelectric coefficient of 1.3 pC/N, similar to human bone [12,37]), this polymer can provide electrical stimulation through mechanical solicitation and with a suitable degradation rate for a variety of tissue engineering applications [38,39].In addition to tissue engineering applications, PHBV has a wide range of industrial applications such as food packaging [40], cosmetics, personal care products (towels and diapers), helmets and panels for several automotive materials [41][42][43]. More recently, this polymer was also used in the scope of environmental remediation towards nitrates and chlorine removal from contaminated water [44,45].A biodegradable magnetoelectric composite, combining piezoelectric PHBV with magnetostrictive cobalt ferrites, CoFe 2 O 4 (CFO) has been reported [8], confirming the ability of biodegradable PHBV and magnetoelectric compound (PHBV/CFO) to be processed into different morphologies, including microspheres, films, fibers and 3D porous scaffolds, adequate for tissue engineering applications with different structural microenvironments.As the biodegradation process occurs, it is important to keep the properties that assure the effectiveness of the scaffold after its implantation.…”

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confidence: 99%

“…In addition to tissue engineering applications, PHBV has a wide range of industrial applications such as food packaging [40], cosmetics, personal care products (towels and diapers), helmets and panels for several automotive materials [41][42][43]. More recently, this polymer was also used in the scope of environmental remediation towards nitrates and chlorine removal from contaminated water [44,45].…”

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confidence: 99%

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Morphology Dependence Degradation of Electro- and Magnetoactive Poly(3-hydroxybutyrate-co-hydroxyvalerate) for Tissue Engineering Applications

et al. 2020

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Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) is a piezoelectric biodegradable and biocompatible polymer suitable for tissue engineering applications. The incorporation of magnetostrictive cobalt ferrites (CFO) into PHBV matrix enables the production of magnetically responsive composites, which proved to be effective in the differentiation of a variety of cells and tissues. In this work, PHBV and PHBV with CFO nanoparticles were produced in the form of films, fibers and porous scaffolds and subjected to an experimental program allowing to evaluate the degradation process under biological conditions for a period up to 8 weeks. The morphology, physical, chemical and thermal properties were evaluated, together with the weight loss of the samples during the in vitro degradation assays. No major changes in the mentioned properties were found, thus proving its applicability for tissue engineering applications. Degradation was apparent from week 4 and onwards, leading to the conclusion that the degradation ratio of the material is suitable for a large range of tissue engineering applications. Further, it was found that the degradation of the samples maintain the biocompatibility of the materials for the pristine polymer, but can lead to cytotoxic effects when the magnetic CFO nanoparticles are exposed, being therefore needed, for magnetoactive applications, to substitute them by biocompatible ferrites, such as an iron oxide (Fe 3 O 4 ).Polymers 2020, 12, 953 2 of 15In particular, smart polymers are gaining increasing attention as substrates and scaffolds for tissue engineering applications mainly as electroactive substrates-mostly piezoelectric ones-such as poly(l-lactic acid) (PLLA) [5,6], poly(hydroxybutyrate) (PHB) [7], poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) [8,9] and poly(vinylidene fluoride) (PVDF) [10,11], among others. The ability of those materials to actively enhance and stimulate cellular differentiation processes has been already proven [12,13], based on their mechano-transduction characteristics, generating voltage upon mechanical stimulation and vice-versa [14]. Piezoelectricity is a property that appears in a diversity of human tissues, including DNA, bones or tendons, emphasizing the relevance of electrical and mechano-electrical stimulation in physiological processes [15,16]. In a different approach to apply mechanical and/or mechano-electrical signals, magnetoelectric materials have also proven their aptness for tissue engineering applications, with the particularity of allowing electrical stimulation of the materials and, therefore, on the cells cultured on them, through magnetic solicitation [17,18]. These materials can generate voltage upon magnetic stimulation through the coupling of the magnetostrictive effect (magnetic to mechanical) and piezoelectric effect (mechanical to electric) [19,20]. Magnetoelectric composites are thus achieved combining a piezoelectric polymer with magnetostrictive particles [21][22][23], and their potential for tissue engineering and enhancement of cellular di...

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confidence: 99%

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confidence: 99%

Morphology Dependence Degradation of Electro- and Magnetoactive Poly(3-hydroxybutyrate-co-hydroxyvalerate) for Tissue Engineering Applications

et al. 2020

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“…Human nasal chondrocytes and human adipose stem cells seeded into gellan gum hydrogels [196] Static hydrostatic Bovine articular chondrocytes [197] Static and pulsed hydrostatic Bovine articular chondrocytes seeded into agarose gels [198] (continued) [199] Rabbit chondrocytes seeded into chitosan/gelatin [200] Rabbit chondrocytes seeded into genipincrosslinked chitosan/collagen [201] KUM5 cells seeded into macro porous poly(ε-caprolactone) [174] Stepper motors displacement, biaxial actuators Some experiments can be found in literature using this functional basic type of bioreactor: for bone tissue enhancing osteoblastic markers within coralline hydroxyapatite scaffolds for human MSCs reaching significant higher cells count for µm pore scaffolds; [151] for cartilage constructs using PLGA scaffolds for rabbit MSCs ensuing in tubular tracheal grafts formation [152] and fibrin gels as scaffolds for human adipose stem cells (ASCs) resulting in chondrogenic differentiation. [153] Spinner flasks have mainly two variation culture setups, first as in batch culture, a closed type of culture mode that disables the addition of fresh medium and waste removal, limiting production but providing a solid method to avoid contamination, whereas the second setup of continuous culture enables waste removal, but puts the system susceptible to potential contaminations.…”

Section: Vascularmentioning

confidence: 99%

“…The most used one is the one providing dynamic stimulation to cells such as cartilage, offering better results by comparison with other types of stimuli for these types of tissues. [174,176] These devices are usually composed by a system of linear vertical movement with the aid of a motor and control system, which manages different amplitudes and frequencies of motor oscillations, as exemplified in Figure 5F.…”

Section: Compression Based Stimulimentioning

confidence: 99%

Physically Active Bioreactors for Tissue Engineering Applications

et al. 2020

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Tissue engineering (TE) is a dynamic and growing scientific field that merges the knowledge of different areas such as biology, physics, medicine, and engineering. [1] The TE discipline was first coined at a National Science Foundation sponsored meeting in 1987, thus originating a field that employs life sciences and engineering with the purpose of developing biologic or synthetic supports to restore, keep, or enhance tissue functions or damaged organs. [2] The classical TE approach has been mainly focusing on associating cells with a supporting matrix, also called biomaterials or scaffolds, that essentially acts as a passive template for tissue formation in vitro by allowing cells to adhere, migrate, differentiate, and produce tissue. Usually, the cells are seeded onto the scaffolds and, occasionally, growth factors are also added. The combination of cells, growth factors, and scaffold is often referred as the TE triad. [3] Nevertheless, novel approaches in TE that include the application of a biophysical stimuli to the scaffolds through the use of a bioreactor are emerging. [4] Tissue engineering (TE) is a strongly expanding research area. TE approaches require biocompatible scaffolds, cells, and different applied stimuli, which altogether mimic the natural tissue microenvironment. Also, the extracellular matrix serves as a structural base for cells and as a source of growth factors and biophysical cues. The 3D characteristics of the microenvironment is one of the most recognized key factors for obtaining specific cell responses in vivo, being the physical cues increasingly investigated. Supporting those advances is the progress of smart and multifunctional materials design, whose properties improve the cell behavior control through the possibility of providing specific chemical and physical stimuli to the cellular environment. In this sense, a varying set of bioreactors that properly stimulate those materials and cells in vitro, creating an appropriate biomimetic microenvironment, is developed to obtain active bioreactors. This review provides a comprehensive overview on the important microenvironments of different cells and tissues, the smart materials type used for providing such microenvironments and the specific bioreactor technologies that allow subjecting the cells/ tissues to the required biomimetic biochemical and biophysical cues. Further, it is shown that microfluidic bioreactors represent a growing and interesting field that hold great promise for achieving suitable TE strategies.

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“…The metabolic requirements of different tissues are varied and dictate the perfusion, gaseous/waste exchange, pH, and mechanical environment required. There is an increasing awareness that molecular and mechanical signaling is pivotal in the growth and differentiation of tissue-engineered constructs, and in addition to well-known growth factors such as bone morphogenetic proteins, vascular epithelial growth factor, basic fibroblast growth factor FGF-2, and transforming growth factor-β ( 65 ), “induction factors,” including oxygen tension ( 66 – 68 ), mechanical ( 69 ), and electrical stimulation ( 70 ), guide subsequent proliferation and differentiation of cells. Cells participate in a web of multidirectional interactions within their niches and tissues of residence [interacting with various nanotopographically sized cues ( 71 )].…”

Section: Introductionmentioning

confidence: 99%

Tissue-Engineered Solutions in Plastic and Reconstructive Surgery: Principles and Practice

et al. 2017

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Recent advances in microsurgery, imaging, and transplantation have led to significant refinements in autologous reconstructive options; however, the morbidity of donor sites remains. This would be eliminated by successful clinical translation of tissue-engineered solutions into surgical practice. Plastic surgeons are uniquely placed to be intrinsically involved in the research and development of laboratory engineered tissues and their subsequent use. In this article, we present an overview of the field of tissue engineering, with the practicing plastic surgeon in mind. The Medical Research Council states that regenerative medicine and tissue engineering “holds the promise of revolutionizing patient care in the twenty-first century.” The UK government highlighted regenerative medicine as one of the key eight great technologies in their industrial strategy worthy of significant investment. The long-term aim of successful biomanufacture to repair composite defects depends on interdisciplinary collaboration between cell biologists, material scientists, engineers, and associated medical specialties; however currently, there is a current lack of coordination in the field as a whole. Barriers to translation are deep rooted at the basic science level, manifested by a lack of consensus on the ideal cell source, scaffold, molecular cues, and environment and manufacturing strategy. There is also insufficient understanding of the long-term safety and durability of tissue-engineered constructs. This review aims to highlight that individualized approaches to the field are not adequate, and research collaboratives will be essential to bring together differing areas of expertise to expedite future clinical translation. The use of tissue engineering in reconstructive surgery would result in a paradigm shift but it is important to maintain realistic expectations. It is generally accepted that it takes 20–30 years from the start of basic science research to clinical utility, demonstrated by contemporary treatments such as bone marrow transplantation. Although great advances have been made in the tissue engineering field, we highlight the barriers that need to be overcome before we see the routine use of tissue-engineered solutions.

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Design and validation of a biomechanical bioreactor for cartilage tissue culture

Cited by 13 publications

References 25 publications

Morphology Dependence Degradation of Electro- and Magnetoactive Poly(3-hydroxybutyrate-co-hydroxyvalerate) for Tissue Engineering Applications

Morphology Dependence Degradation of Electro- and Magnetoactive Poly(3-hydroxybutyrate-co-hydroxyvalerate) for Tissue Engineering Applications

Physically Active Bioreactors for Tissue Engineering Applications

Tissue-Engineered Solutions in Plastic and Reconstructive Surgery: Principles and Practice

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