Design and validation of a real-time PCR technique for assessing the level of inclusion of fungus- and yeast-based additives in feeds

Rinttilä, Teemu; Ülle, Kir; Apajalahti, Juha H. A.; Timmons, Rebecca; Moran, C. A.

doi:10.1016/j.mimet.2020.105867

Cited by 1 publication

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amplification, standard, and melt curves for unpublished primers are provided in Supplementary Figure S1 . The qPCR assay development and optimization were conducted as described in Rinttilä et al (2020) . The thermal cycling conditions involved one cycle of preheating at 50°C for 2 min and an initial denaturation at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealing and extension at the primer-specific annealing temperatures for 60s.…”

Section: Methodsmentioning

confidence: 99%

Stimbiotic supplementation and xylose-rich carbohydrates modulate broiler’s capacity to ferment fibre

Davies,

González-Ortiz,

Rinttilä

et al. 2024

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Stimbiotics are a new category of feed additives that can increase fibre fermentability by stimulating fibre-degrading microbiota in the gut. The aim of this study was to test, ex vivo, if the microbiota of broilers fed a stimbiotic are better able to ferment different xylose-rich substrates in an ileal and a caecal environment. The ileal and caecal contents from broiler chickens fed a stimbiotic or from a control group were used as an inoculum in the ex vivo fermentation experiment. Different xylose-rich substrates including monomeric xylose (XYL), XOS with DP 2 to 6 (XOS), short DP XOS of 2 to 3 (sDP-XOS), long DP XOS of 4 to 6 (lDP-XOS) and de-starched wheat bran (WB), were added to each ileal and caecal inoculum in fermentation vessels. Total gas, short-chain fatty acids (SCFA) production, bacterial quantification, and carbohydrate utilisation were monitored for 9 h post-inoculation. No significant interactions were observed in any of the parameters measured in either the ileal or caecal contents (p > 0.05). Stimbiotic ileal inocula resulted in higher total gas (p < 0.001) and volatile fatty acid (VFA) (p < 0.001) production, increased numbers of Lactobacillus spp. (p < 0.001), and decreased numbers of Enterococcus spp. (p < 0.01) after 9 h regardless of the xylose-rich substrate added. Stimbiotic caecal inocula resulted in a higher ratio of VFA to branched-chain fatty acids (BCFAs) by up to +9% (p < 0.05). Ileal microbiota were found to preferentially metabolise WB, while caecal microbiota favoured XOS substrates, particularly lDP-XOS. These results indicate that stimbiotics can promote the abundance of lactic acid bacteria involved in the establishment of fibre-degrading bacteria and VFA content in the gut, which could have beneficial effects on broiler performance. Further, ileal and caecal microbiota differ in their utilisation of different substrates which may impact the effectiveness of different stimbiotic products.

show abstract

Section: Methodsmentioning

confidence: 99%