Design and validation of an ELISA for equine infectious anemia (EIA) diagnosis using synthetic peptides

Soutullo, Adriana R.; Verwimp, Verónica; Riveros, Mario; Pauli, R.; Tonarelli, Georgina

doi:10.1016/s0378-1135(00)00352-7

Cited by 27 publications

(23 citation statements)

References 27 publications

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“…The major drawback of this test is that it is time consuming and sometimes difficult to interpret. Different tests based on a competitive or non-competitive enzyme-linked immunosorbent assay (ELISA) have been developed and compared to the "Coggins test" [12,37,84,160]. Recently, an EIAV diagnostic assay based on fluorescence polarization (FP) using a peptide derived from the gp45 transmembrane glycoprotein has been developed [172].…”

Section: Control Of Eiav Infectionmentioning

confidence: 99%

Equine Infectious Anemia Virus (EIAV): what has HIV?s country cousin got to tell us?

2004

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-Equine Infectious Anemia Virus (EIAV) is a lentivirus, of the Retrovirus family, with an almost worldwide distribution, infecting equids. It causes a persistent infection characterized by recurring febrile episodes associating viremia, fever, thrombocytopenia, and wasting symptoms. The disease is experimentally reproducible by inoculation of Shetland ponies or horses with EIAV pathogenic strains. Among lentiviruses, EIAV is unique in that, despite a rapid virus replication and antigenic variation, most animals progress from a chronic stage characterized by recurring peaks of viremia and fever to an asymptomatic stage of infection. The inapparent carriers remain infective for life, as demonstrated by experimental transfer of blood to naive animals. The understanding of the correlates of this immune control is of great interest in defining vaccine strategies. Research on EIAV, this "country cousin" of HIV (Human Immunodeficiency Virus), over the last five decades has produced some interesting results on natural immunological control of lentivirus replication and disease and on the nature and role of virus variation in persistence and pathogenesis. These studies are of interest in the context of HIV and efforts to develop a vaccine. This review will focus on some of the most recent results. equine infectious anemia virus / lentivirus / equids / vaccine / viral evolution

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Section: Control Of Eiav Infectionmentioning

confidence: 99%

Equine Infectious Anemia Virus (EIAV): what has HIV?s country cousin got to tell us?

2004

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“…In an effort to develop a more rapid and sensitive diagnostic capability, several attempts have been made on development of enzyme-linked immunosorbent assays (ELISAs) based on p26 protein purified from EIAV strain [28,29,32], recombinant proteins [1,2,15,22,25,33] and peptides [27,31]. The EIAV p26 protein encoded by gag gene is one of the highly conserved structural proteins among the viral isolates even from very different geographical locations [4,5,26].…”

Section: Discussionmentioning

confidence: 99%

“…However, recent emergence of EIAV infection in Asian continent like, Japan [17], Mongolia [20], China, Thailand, Uzbekistan, Philippines, and Malaysia during [2008][2009][2010][2011][2012] [http://www.oie.int/wahis_2/public/wahid.php/Wahidhome] and detection of EIAV infection in 2010 and 2012 in India [16] after a long gap underlines the importance of continued surveillance to monitor the disease prevalence and identifying the genetic variation among field strains of EIAV. During the last two decades, the detection of EIAV antibodies by a number of ELISAs has been described and compared with 'gold standard' agar gel immuno-diffusion (AGID) test [3,21,22,24,25,28,29,31,32]. The ELISA has been reported to be more sensitive than AGID in detecting acute cases of EIA and helpful in disease eradication programme [10].…”

Section: Introductionmentioning

confidence: 99%

Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus

et al. 2013

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Equine infectious anemia (EIA)-a retroviral disease caused by equine infectious anemia virus (EIAV)-is a chronic, debilitating disease of horses, mules, and donkeys. EIAV infection has been reported worldwide and is recognized as pathogen of significant economic importance to the horse industry. This disease falls under regulatory control program in many countries including India. Control of EIA is based on identification of inapparent carriers by detection of antibodies to EIAV in serologic tests and ''Stamping Out'' policy. The current internationally accepted test for diagnosis of EIA is the agar gel immune-diffusion test (AGID), which detects antibodies to the major gag gene (p26) product. The objective of this study was to develop recombinant p26 based in-house immunoassays [enzyme linked immunosorbent assays (ELISA), and AGID] for EIA diagnosis. The synthetic p26 gene of EIAV was expressed in Escherichia coli and diagnostic potential of recombinant p26 protein were evaluated in ELISA and AGID on 7,150 and 1,200 equine serum samples, respectively, and compared with commercial standard AGID kit. The relative sensitivity and specificity of the newly developed ELISA were 100 and 98.6 %, respectively. Whereas, relative sensitivity and specificity of the newly developed AGID were in complete agreement in respect to commercial AGID kit. Here, we have reported the validation of an ELISA and AGID on large number of equine serum samples using recombinant p26 protein produced from synthetic gene which does not require handling of pathogenic EIAV. Since the indigenously developed reagents would be economical than commercial diagnostic kit, the rp26 basedimmunoassays could be adopted for the sero-diagnosis and control of EIA in India.

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“…Read the absorbance at appropriate wavelength with an appropriate time resolved plate reader. Soutullo et al (2001) evaluated the performance of an equine infectious an Aemia-ELISA designed with synthetic peptides. This assay could be important to prove for large throughput screening and early detection of equine infectious anaemia (EIA), when the results of the traditional Coggins test are still negative.…”

Section: Peptide Based-elisamentioning

confidence: 99%

Biotechnological tools for diagnosis of equine infectious diseases

Prasad¹,

Brar²,

Ikbal³

et al. 2016

JEBAS

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Rapid diagnosis of infectious diseases and appropriate treatment with in time are important steps that promote optimal clinical outcomes and general public health. Today there is large number of new technologies such as nanotechnology, biosensors, and microarray techniques, are being developed and used as diagnostic tools for equine infectious diseases. Nucleic acid based techniques such as polymerase chain reaction (PCR) have become conventional tools in veterinary research and plays an important role in specific typing determinations as well as for rapid screening of ample numbers of samples at the time of equine disease outbreaks. Other biotechnological techniques are populous to be used in the coming times as they can enhance diagnostic efficacy in less time and cost as compared to conventional techniques. This review focuses on biotechnological tools available for equine diseases diagnosis and its applications hold great promise for improving the speed and accuracy of diagnostics for equine infectious diseases.

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Design and validation of an ELISA for equine infectious anemia (EIA) diagnosis using synthetic peptides

Cited by 27 publications

References 27 publications

Equine Infectious Anemia Virus (EIAV): what has HIV?s country cousin got to tell us?

Equine Infectious Anemia Virus (EIAV): what has HIV?s country cousin got to tell us?

Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus

Biotechnological tools for diagnosis of equine infectious diseases

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