Design, application, and microbiome of sulfate-reducing bioreactors for treatment of mining-influenced water

Habe, Hiroshi; Sato, Yuya; Aoyagi, Tomo; Inaba, Tomohiro; Hori, Tomoyuki; Hamai, Takaya; Hayashi, Kentaro; Kobayashi, Mikio; Sakata, Takeshi; Sato, Naoki

doi:10.1007/s00253-020-10737-2

Cited by 27 publications

(6 citation statements)

References 47 publications

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“…Therefore, for AMD remediation, bacterial sulfate reduction in CWs is a key process as it reduces the acidity of AMD and removes heavy metals by immobilizing them as sulfides, hydroxides, and carbonate precipitates ( Chen et al, 2021c ). The bacteria involved in sulfate reduction are known as sulfate-reducing bacteria (SRB) and they can drive simultaneous sulfate and metal removal as well as acidity neutralization ( Habe et al, 2020 ; Chen et al, 2021b ). The majority of SRB belong to the class Deltaproteobacteria in the phylum Proteobacteria .…”

Section: Functional Microorganisms In Heavy Metal Removalmentioning

confidence: 99%

A Review on Microorganisms in Constructed Wetlands for Typical Pollutant Removal: Species, Function, and Diversity

Wang

Long

et al. 2022

Front. Microbiol.

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Constructed wetlands (CWs) have been proven as a reliable alternative to traditional wastewater treatment technologies. Microorganisms in CWs, as an important component, play a key role in processes such as pollutant degradation and nutrient transformation. Therefore, an in-depth analysis of the community structure and diversity of microorganisms, especially for functional microorganisms, in CWs is important to understand its performance patterns and explore optimized strategies. With advances in molecular biotechnology, it is now possible to analyze and study microbial communities and species composition in complex environments. This review performed bibliometric analysis of microbial studies in CWs to evaluate research trends and identify the most studied pollutants. On this basis, the main functional microorganisms of CWs involved in the removal of these pollutants are summarized, and the effects of these pollutants on microbial diversity are investigated. The result showed that the main phylum involved in functional microorganisms in CWs include Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. These functional microorganisms can remove pollutants from CWs by catalyzing chemical reactions, biodegradation, biosorption, and supporting plant growth, etc. Regarding microbial alpha diversity, heavy metals and high concentrations of nitrogen and phosphorus significantly reduce microbial richness and diversity, whereas antibiotics can cause large fluctuations in alpha diversity. Overall, this review can provide new ideas and directions for the research of microorganisms in CWs.

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Section: Functional Microorganisms In Heavy Metal Removalmentioning

confidence: 99%

A Review on Microorganisms in Constructed Wetlands for Typical Pollutant Removal: Species, Function, and Diversity

Wang

Long

et al. 2022

Front. Microbiol.

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“…Despite its promise, few ARD-remediating BSR bioreactors have been implemented and operated at scale, with the exception of passive systems with extended hydraulic retention time. 14 The successful operation of BSR reactors faces several challenges, including the efficient utilization of the supplied electron donor due to competition between the SRM and other microorganisms within the microbial community. Further, achieving high reaction rates by overcoming the relatively low growth rates of SRM has been difficult.…”

Section: Introductionmentioning

confidence: 99%

Harnessing Fermentation May Enhance the Performance of Biological Sulfate-Reducing Bioreactors

Hessler,

Harrison,

Banfield

et al. 2024

Environ. Sci. Technol.

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Biological sulfate reduction (BSR) represents a promising strategy for bioremediation of sulfate-rich waste streams, yet the impact of metabolic interactions on performance is largely unexplored. Here, genome-resolved metagenomics was used to characterize 17 microbial communities in reactors treating synthetic sulfate-contaminated solutions. Reactors were supplemented with lactate or acetate and a small amount of fermentable substrate. Of the 163 genomes representing all the abundant bacteria, 130 encode 321 NiFe and FeFe hydrogenases and all genomes of the 22 sulfate-reducing microorganisms (SRM) encode genes for H 2 uptake. We observed lactate oxidation solely in the first packed bed reactor zone, with propionate and acetate oxidation in the middle and predominantly acetate oxidation in the effluent zone. The energetics of these reactions are very different, yet sulfate reduction kinetics were unaffected by the type of electron donor available. We hypothesize that the comparable rates, despite the typically slow growth of SRM on acetate, are a result of the consumption of H 2 generated by fermentation. This is supported by the sustained performance of a predominantly acetate-supplemented stirred tank reactor dominated by diverse fermentative bacteria encoding FeFe hydrogenase genes and SRM capable of acetate and hydrogen consumption and CO 2 assimilation. Thus, addition of fermentable substrates to stimulate syntrophic relationships may improve the performance of BSR reactors supplemented with inexpensive acetate.

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“…In a microbiome study on microalgal piggery wastewater treatment, no pathogens were detected because the feedstock was obtained from stabilization lagoons [ 16 ]. Although relationships between microalgae and bacteria in wastewater are poorly understood because of the complexity of the interactions between biota [ 17 ], recent developments in statistical correlation analyses have enabled the elucidation of microbial communities at higher resolution and their potential interactions [ 18 ]. As pathogens and microbial changes are an emerging issue [ 19 ] and biological treatment causes a microbial community change, metagenome analysis is required to investigate changes in pathogenic bacteria during the microalgal piggery wastewater treatment process.…”

Section: Introductionmentioning

confidence: 99%

Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

Lee

Kim

et al. 2022

Microbiome

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Background Monitoring microbial communities especially focused on pathogens in newly developed wastewater treatment systems is recommended for public health. Thus, we investigated the microbial community shift in a pilot-scale microalgal treatment system for piggery wastewater. Results Microalgae showed reasonable removal efficiencies for COD and ammonia, resulting in higher transparency of the final effluent. Metagenome and microbial diversity analyses showed that heterotrophic microalgal cultivation barely changed the bacterial community; however, the mixotrophic microalgal cultivation induced a sudden change. In addition, an evaluation of risk groups (RGs) of bacteria showed that raw piggery wastewater included abundant pathogens, and the microalgal treatment of the raw piggery wastewater decreased the RG2 pathogens by 63%. However, co-cultivation of microalgae and the most dominant RG2 pathogen, Oligella, showed no direct effects between them. Conclusions Thus, a microbial interaction network was constructed to elucidate algae-bacteria interrelationships, and the decrease in Oligella was indirectly connected with microalgal growth via Brevundimonas, Sphingopyxis, and Stenotrophomonas. In a validation test, 3 among 4 connecting bacterial strains exhibited inhibition zones against Oligella. Therefore, we showed that microalgal wastewater treatment causes a decrease in RG2 bacteria, which is an indirect impact of microalgae associated with bacteria. Graphical Abstract

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Design, application, and microbiome of sulfate-reducing bioreactors for treatment of mining-influenced water

Cited by 27 publications

References 47 publications

A Review on Microorganisms in Constructed Wetlands for Typical Pollutant Removal: Species, Function, and Diversity

A Review on Microorganisms in Constructed Wetlands for Typical Pollutant Removal: Species, Function, and Diversity

Harnessing Fermentation May Enhance the Performance of Biological Sulfate-Reducing Bioreactors

Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction

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