Design, characterization and comparison of materials based on β and γ cyclodextrin covalently connected to microporous silica for environmental analysis

Belenguer-Sapiña, Carolina; Pellicer-Castell, Enric; Haskouri, Jamal El; Guillem, Carmen; Simó‐Alfonso, Ernesto Francisco; Amorós, Pedro; Mauri-Aucejo, Adela R.

doi:10.1016/j.chroma.2018.05.070

Cited by 19 publications

(4 citation statements)

References 35 publications

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“…As for the extraction performances of MWCNT-H 2 O 2 -γ-CD and MWCNT-H 2 O 2 -β-CD, better results were obtained using the nanocomposite functionalized with γ-CD, thus assuming that this receptor is able to complex PAHs more effectively. These findings are consistent with those reported by Belenguer-Sapiña et al [ 17 ], who developed silica structures containing bound CDs for the determination of phenolic compounds in air and PAHs in water samples. As already demonstrated, the complementarity of the cavity size with the steric hindrance of the analytes plays a key role in promoting their extraction from water: β-CD and γ-CD have cavity diameters of 0.78 and 0.95 nm, respectively, while PAHs have volumes in the 0.8–0.9 nm range.…”

Section: Resultssupporting

confidence: 93%

“…CDs can form host-guest complexes with different compounds, such as dyes, ions, and volatile and semivolatile organic compounds: in particular, both β- and γ-CDs demonstrated excellent inclusion performances towards polycyclic aromatic hydrocarbons (PAHs) [ 17 – 21 ]. Other advantageous features rely on the low price compared to other designed receptors, negligible environmental impact, and near-zero toxicity.…”

Section: Introductionmentioning

confidence: 99%

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A novel multiwalled carbon nanotube–cyclodextrin nanocomposite for solid-phase microextraction–gas chromatography–mass spectrometry determination of polycyclic aromatic hydrocarbons in snow samples

et al. 2023

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Novel solid-phase microextraction coatings based on the use of multiwalled carbon nanotube–cyclodextrin (MWCNT-CD) nanocomposites were developed for the determination of 16-priority polycyclic aromatic hydrocarbons at ultratrace levels in snow samples. The performance of both β- and γ-CD was tested to increase the detection capabilities towards the heaviest and most lipophilic compounds, i.e., five- and six-ring PAHs. To facilitate the interactions of MWCNTs with CDs, an oxidation procedure using both HNO3 and H2O2 was applied, obtaining superior results using MWCNTs-H2O2-γ-CD fiber. Detection and quantitation limits below 0.7 and 2.3 ng/L, RSD lower than 21%, and recoveries of 88(± 2)–119.8(± 0.4)% proved the reliability of the developed method for the determination of PAHs at ultratrace levels. The complexation capability of the γ-CD was also demonstrated in solution by NMR and fluorescence spectroscopy studies and at solid state by XRD analysis. Finally, snow samples collected in the ski area of Dolomiti di Brenta were analyzed, showing a different distribution of the 16 priority PAHs, being naphthalene, phenanthrene, fluoranthene, and pyrene the only compounds detected in all the analyzed samples. Graphical Abstract

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

A novel multiwalled carbon nanotube–cyclodextrin nanocomposite for solid-phase microextraction–gas chromatography–mass spectrometry determination of polycyclic aromatic hydrocarbons in snow samples

et al. 2023

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“…The characterization of the interactions between chitosanases and affinity resins was carried out using equilibrium adsorption study. Scatchard analysis model was used for analysis of the desorption constant ( K d ) and the theoretical maximum adsorption capacity ( Q max ) of different affinity resins [22,29]. Various concentrations of enzymes (10 mL, 0.1–0.9 mg/mL in 20 mM Tris-HCl buffer, pH 8.0) were combined with 5 g of each type of resin to reach the adsorption equilibrium in a shaken condition.…”

Section: Methodsmentioning

confidence: 99%

Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification

Wang

Chen

et al. 2019

Marine Drugs

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Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer, cyanuric chloride. Based on the CHDS-modified affinity resin, a one-step affinity purification method was developed and optimized, and then applied to purify three typical glycoside hydrolase (GH) families: 46, 75, and 80 chitosanase. The three purified chitosanases were homogeneous with purities of greater than 95% and bioactivity recovery of more than 40%. Moreover, we also developed a rapid and efficient affinity purification procedure, in which tag-free chitosanase could be directly purified from supernatant of bacterial culture. The purified chitosanases samples using such a procedure had apparent homogeneity, with more than 90% purity and 10–50% yield. The novel purification methods established in this work can be applied to purify native chitosanases in various scales, such as laboratory and industrial scales.

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“…Conventional column techniques using commercially available octadecylsilyl (ODS) or C18-bonded phases are probably the most widespread method used to date, which can attain high enrichment factors by extracting PAHs from large-volume samples [5][6][7][8][9][10][11][12]. Novel materials for column packing, such as multiwalled carbon nanotubes or graphene [13][14][15][16], magnesium oxide microspheres [17], molecularly imprinted polymers [18], bound cyclodextrin-silica phase [19,20], and biosorbents [21,22], have been developed and used for the extraction of PAHs. Nevertheless, the disadvantages of conventional column techniques, such as the requirement of several steps to complete the enrichment process, the use of several milliliters of eluent, and possible contamination and loss resulting from the processing of large-volume sample solutions, have led researchers to develop new micro-SPE methods [3,4,[23][24][25][26][27][28].…”

mentioning

confidence: 99%

A thin‐layer solid‐phase extraction–liquid film elution technique used for the enrichment of polycyclic aromatic hydrocarbons in water

Nukatsuka

Satoh

Kihara

et al. 2021

J of Separation Science

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In this article, we propose a novel microsolid-phase extraction and elution technique, which we called the thin-layer solid-phase extraction-liquid film elution technique. The thin-layer solid-phase extraction phase is an octadecylsilylated sol gel-coated porous silica thin film prepared on the outer wall of a test tube, which has a larger surface area for the extraction of the target compounds compared to a conventional solid-phase microextraction phase. After optimization of the extraction procedure for five types of polycyclic aromatic hydrocarbons, the liquid film elution technique was investigated. Liquid film elution is an elution technique wherein the compounds extracted into the thin-layer solid-phase extraction phase are eluted using a small volume of solvent film formed around the extraction phase. The results show that the elution can be carried out using 150 μL of eluent. Enrichment factors between 20 and 34 were obtained for polycyclic aromatic hydrocarbons containing more than four aromatic rings in 10 mL aliquots of aqueous samples. Finally, recoveries of 85-112% were obtained for polycyclic aromatic hydrocarbons containing more than four aromatic rings from spiked natural water samples using the thin-layer solid-phase extraction-liquid film elution technique.

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Design, characterization and comparison of materials based on β and γ cyclodextrin covalently connected to microporous silica for environmental analysis

Cited by 19 publications

References 35 publications

A novel multiwalled carbon nanotube–cyclodextrin nanocomposite for solid-phase microextraction–gas chromatography–mass spectrometry determination of polycyclic aromatic hydrocarbons in snow samples

A novel multiwalled carbon nanotube–cyclodextrin nanocomposite for solid-phase microextraction–gas chromatography–mass spectrometry determination of polycyclic aromatic hydrocarbons in snow samples

Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification

A thin‐layer solid‐phase extraction–liquid film elution technique used for the enrichment of polycyclic aromatic hydrocarbons in water

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