Design, construction and optimization a flexible bench-scale rotating biological contactor (RBC) for enhanced production of bacterial cellulose by Acetobacter Xylinium

Soleimani, Ali; Hamedi, Sepideh; Babaeipour, Valiollah; Rouhi, Motahreh

doi:10.1007/s00449-021-02510-0

Cited by 12 publications

(16 citation statements)

References 35 publications

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“…Molasses is one of the most important wastes of the food industry that is used in the development of economic culture medium and has a complex composition that includes a high amount of inhibitory substances. 17 Therefore, pre-treatment with various enzymatic or chemical processes is necessary to detoxify the inhibitory compounds and potentially provide hydrolysis with a high concentration of reducing sugars. 18 The raw molasses was treated according to the method of Bae and Shoda as follows.…”

Section: Molasses Treatmentmentioning

confidence: 99%

“…14,15 Various solutions have been used, including the design and optimization of cheap culture media, the development of industrial strains with higher cellulose production capabilities, the optimization of fermentation conditions and the type of cultivation process, and the use of various bioreactors to reduce the cost of BC production. [13][14][15][16][17] The development of cheap culture media with the ability to produce higher and faster BC is a solution that has been more considered due to its greater impact on the cost of production. 14,18 The development of an efficient and cheap culture medium requires investigating the effect of various factors affecting BC production, including the type and concentration of culture medium components (carbon source, nitrogen source, and trace elements), pH, oxygen concentration, and agitation for each BC-producing strain.…”

Section: Introductionmentioning

confidence: 99%

“…14,18 The development of an efficient and cheap culture medium requires investigating the effect of various factors affecting BC production, including the type and concentration of culture medium components (carbon source, nitrogen source, and trace elements), pH, oxygen concentration, and agitation for each BC-producing strain. 4,17,18 HS culture medium is the most preferred culture medium for BC production on a lab scale, which is not suitable for industrial BC production. Therefore, in recent years, many efforts have been made to reduce the costs of the cultivation medium by modifying or changing the HS culture medium.…”

Section: Introductionmentioning

confidence: 99%

“…The choice of cultivation method depends on the required characteristics such as physical, morphological, and mechanical characteristics of the final product. 17,22,23 Static culture is a simple and widely used method for BC production because cellulose is produced in containers with a large air-liquid surface, which supports a direct oxygen supply for BC production. BC production in this situation is related to the surface-tovolume ratio of culture medium.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes

Khanchezar,

Babaeipour,

Mostafa

2024

Biotech and App Biochem

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In this study, a cost‐effective complex culture media containing molasses and corn steep liquor (CSL) was developed for the high production of bacterial cellulose (BC) by investigating the effect of four effective factors on BC production at three levels using Taguchi and combined methods. The predicted and actual values of BC production in optimal conditions by Taguchi and combined methods were 8.41 and 14.52 g/L, respectively. These results showed that the combined method was more suitable for predicting the optimal conditions in the optimization of BC production, the cost of developed culture medium was around 94% cost of HS medium preparation, molasses was the most effective factor in both experimental design methods, and initial pH adjustment had little impact on BC production. Then, the effect of inoculation conditions containing three factors of inoculation age, ethanol addition time, and agitation rate on the increase of BC production at three levels was investigated using the response surface methodology with the Box–Behnken design algorithm. Under the optimal conditions including inoculum age of 3 days, ethanol addition time of 10 days, and stirring speed of 100 rpm, the predicted and experimental results of BC production were 21.61 and 20.21 g/L, respectively. This is among the highest ever reported for BC production, which was achieved with a more cost‐effective culture medium containing molasses and CSL.

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Section: Molasses Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes

Khanchezar,

Babaeipour,

Mostafa

2024

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

“…These bioreactors are advantageous because they do not offer shear stress to the growing organisms, and with improved design, these reactors help increase the BC yield [303]. Hamedi et al [300] reported BC fermentation by using Acetobacter xylinum in a rotary biofilm contractor. It was observed that the production rate of BC is affected by the rotational speed of disks, the distance between two disks, and disc media.…”

Section: Rotating Disc Reactormentioning

confidence: 99%

Biomedical engineering aspects of nanocellulose: a review

Rai

Dhar

2022

Nanotechnology

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Cellulose is one of the most abundant renewable biopolymer in nature and is present as major constituent in both plant cell walls as well as synthesized by some microorganisms as extracellular products. In both the systems, cellulose self-assembles into a hierarchical ordered architecture to form micro to nano-fibrillated structures, on basis of which it is classified into various forms. Nanocellulose (NCs) exist as rod-shaped highly crystalline cellulose nanocrystals (CNC) to high aspect ratio cellulose nanofibers (CNF), micro-fibrillated cellulose (MFC) and bacterial cellulose (BC), depending upon the origin, structural and morphological properties. Moreover, NCs have been processed into diversified products ranging from composite films, coatings, hydrogels, aerogels, xerogels, organogels, rheological modifiers, optically active birefringent colored films using traditional-to-advanced manufacturing techniques. With such versatility in structure-property, NCs have profound application in areas of healthcare, packaging, cosmetics, energy, food, electronics, bioremediation, and biomedicine with promising commercial potential. Herein this review, we highlight the recent advancements in synthesis, fabrication, processing of NCs, with strategic chemical modification routes to tailor its properties for targeted biomedical applications. We also study the basic mechanism and models for biosynthesis of cellulose in both plant and microbial systems and understand the structural insights of NC polymorphism. The kinetics study for both enzymatic/chemical modifications of NCs and microbial growth behavior of BC under various reactor configurations are studied. The challenges associated with the commercial aspects as well as industrial scale production of pristine and functionalized NCs to meet the growing demands of market are discussed and prospective strategies to mitigate them are described. Finally, post chemical modification evaluation of biological and inherent properties of NC are important to determine their efficacy for development of various products and technologies directed for biomedical applications.

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Bacterial nanocellulose: A versatile biopolymer production using a cost‐effective wooden disc based rotary reactor

Jagtap,

Dastager

2024

Biopolymers

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Bacterial nanocellulose (BNC) has various unique qualities, including high mechanical strength, crystallinity, and high water‐holding capacity, which makes it appropriate for a wide range of industrial applications. But its lower yield coupled with its high production cost creates a barrier to its usage. In this study, we have demonstrated the better yield of BNC from an indigenous strain Komagataeibacter rhaeticus MCC‐0157 using a rotary disc bioreactor (RDB) having a wooden disc. The RDB was optimized based on the type of disc material, distance between the disc, and rotation speed to get the highest yield of 13.0 g/L dry material using Hestrin–Schramm (H–S) medium. Further, the bioreactor was compared for the BNC production using reported medium, which is used for static condition; the RDB showed up to fivefold increase in comparison with the static condition reported. Komagataeibacter rhaeticus MCC‐0157 was previously reported to be one of the highest BNC producing stains, with 8.37 g/L of dry yield in static condition in 15 days incubation. The designed RDB demonstrated 13.0 g/L dry yield of BNC in just 5 days. Other characteristics of BNC remain same as compared with static BNC production, although the difference in the crystallinity index was observed in RDB (84.44%) in comparison with static (89.74%). For the first time, wooden disc was used for rotary bioreactor approach, which demonstrated higher yield of BNC in lesser time and can be further used for sustainable production of BNC at the industrial level.

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Design, construction and optimization a flexible bench-scale rotating biological contactor (RBC) for enhanced production of bacterial cellulose by Acetobacter Xylinium

Cited by 12 publications

References 35 publications

Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes

Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes

Biomedical engineering aspects of nanocellulose: a review

Bacterial nanocellulose: A versatile biopolymer production using a cost‐effective wooden disc based rotary reactor

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