In this paper a high-gain observer used to estimate the product compositions in a distillation column for a non-ideal mixture (ethanol-water) through the tray temperature measurements is presented. The design of this observer is based on a simplified mathematical model. One of the main advantages of this observer is its constant gain, therefore its tuning depends only on choosing a few constant parameters satisfying some simple algebraic inequalities. The effectiveness of the proposed method is demonstrated through on-line experiments in a distillation pilot plant.