Microscope objectives with wide field-of-view (FOV) and high resolution are urgently needed for the frontier research in life sciences. However, traditional transmission microscope objectives typically have a narrow FOV and severe chromatic aberration. A new off-axis reflective microscope objective with a centimeter scale FOV and micron resolution is proposed in this paper. This objective, with its simple structure, can operate over a wide wavelength range. A design method for a wide FOV optical system is presented, which can eliminate the obstruction of the rays and control the intermediate image plane. Using this method, we design a novel off-axis four-mirror microscope objective with a FOV of 10 mm × 1.5 mm and a numerical aperture of 0.33.